Detection of alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry.
HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC1368G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.
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