Human/Mouse alpha -Fetoprotein/AFP Antibody

(17 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 189502
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Simple Western
    5 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • Intracellular Staining by Flow Cytometry
    0.25 µg/106 cells
    See below
  • Knockout Validated
    alpha ‑Fetoprotein/AFP is specifically detected in HepG2 human hepatocellular carcinoma parental cell line but is not detectable in alpha ‑Fetoprotein/AFP knockout HepG2 cell line.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of alpha ‑Fetoprotein/AFP by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse
alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line. alpha ‑Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Intracellular Staining by Flow Cytometry
Detection of alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Human alpha ‑Fetoprotein/AFP by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Knockout Validated
Western Blot Shows Human alpha ‑Fetoprotein/AFP Specificity by Using Knockout Cell Line. Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and alpha ‑Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) in the parental HeLacell line, but is not detectable in knockout HeLa cell line. GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha-Fetoprotein/AFP
alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.
  • Entrez Gene IDs:
    174 (Human); 11576 (Mouse); 24177 (Rat)
  • Alternate Names:
    AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Establishment of induced pluripotent stem cells from centenarians for neurodegenerative disease research.
    Authors: Yagi T, Kosakai A, Ito D, Okada Y, Akamatsu W, Nihei Y, Nabetani A, Ishikawa F, Arai Y, Hirose N, Okano H, Suzuki N
    PLoS ONE, 2102;7(7):e41572.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  2. One Year Follow-Up Risk Assessment in SKH-1 Mice and Wounds Treated with an Argon Plasma Jet
    Authors: A Schmidt, TV Woedtke, J Stenzel, T Lindner, S Polei, B Vollmar, S Bekeschus
    Int J Mol Sci, 2017;18(4):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease
    Sci Rep, 2016;6(0):32382.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - Not specified
  4. Generation of Naive Bovine Induced Pluripotent Stem Cells Using PiggyBac Transposition of Doxycycline-Inducible Transcription Factors.
    Authors: Kawaguchi T, Tsukiyama T, Kimura K, Matsuyama S, Minami N, Yamada M, Imai H
    PLoS ONE, 2015;10(8):e0135403.
    Species: Bovine
    Sample Type: Whole Cells
    Application: IHC Not Specified
  5. Controlled Growth and the Maintenance of Human Pluripotent Stem Cells by Cultivation with Defined Medium on Extracellular Matrix-Coated Micropatterned Dishes.
    Authors: Takenaka C, Miyajima H, Yoda Y, Imazato H, Yamamoto T, Gomi S, Ohshima Y, Kagawa K, Sasaki T, Kawamata S
    PLoS ONE, 2015;10(6):e0129855.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  6. Lin28b is sufficient to drive liver cancer and necessary for its maintenance in murine models.
    Authors: Nguyen L, Robinton D, Seligson M, Wu L, Li L, Rakheja D, Comerford S, Ramezani S, Sun X, Parikh M, Yang E, Powers J, Shinoda G, Shah S, Hammer R, Daley G, Zhu H
    Cancer Cell, 2014;26(2):248-61.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  7. Generation of human induced pluripotent stem (Ips) cells in serum- and feeder-free defined culture and TGF-Beta1 regulation of pluripotency.
    Authors: Yamasaki S, Taguchi Y, Shimamoto A, Mukasa H, Tahara H, Okamoto T
    PLoS ONE, 2014;9(1):e87151.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  8. Cell-autonomous correction of ring chromosomes in human induced pluripotent stem cells.
    Authors: Bershteyn, Marina, Hayashi, Yohei, Desachy, Guillaum, Hsiao, Edward C, Sami, Salma, Tsang, Kathryn, Weiss, Lauren A, Kriegstein, Arnold R, Yamanaka, Shinya, Wynshaw-Boris, Anthony
    Nature, 2014;507(7490):99-103.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC Fresh
  9. Enzyme-free passage of human pluripotent stem cells by controlling divalent cations.
    Authors: Ohnuma, Kiyoshi, Fujiki, Ayaka, Yanagihara, Kana, Tachikawa, Saoko, Hayashi, Yohei, Ito, Yuzuru, Onuma, Yasuko, Chan, Techuan, Michiue, Tatsuo, Furue, Miho K, Asashima, Makoto
    Sci Rep, 2014;4(0):4646.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC
  10. Identification of transcription factors for lineage-specific ESC differentiation.
    Authors: Yamamizu K, Piao Y, Sharov A, Zsiros V, Yu H, Nakazawa K, Schlessinger D, Ko M
    Stem Cell Reports, 2013;1(6):545-59.
  11. Differentiation and transplantation of functional pancreatic beta cells generated from induced pluripotent stem cells derived from a type 1 diabetes mouse model.
    Authors: Jeon, Kilsoo, Lim, Hyejin, Kim, Jung-Hyu, Thuan, Nguyen V, Park, Seung Hw, Lim, Yu-Mi, Choi, Hye-Yeon, Lee, Eung-Ryo, Kim, Jin-Hoi, Lee, Myung-Sh, Cho, Ssang-Go
    Stem Cells Dev, 2012;21(14):2642-55.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  12. Small RNA-mediated regulation of iPS cell generation.
    Authors: Li Z, Yang CS, Nakashima K, Rana TM
    EMBO J., 2011;30(5):823-34.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  13. Induction of pluripotent stem cells from human third molar mesenchymal stromal cells.
    Authors: Oda Y, Yoshimura Y, Ohnishi H
    J. Biol. Chem., 2010;285(38):29270-8.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  14. Effective generation of iPS cells from CD34+ cord blood cells by inhibition of p53.
    Authors: Takenaka C, Nishishita N, Takada N, Jakt LM, Kawamata S
    Exp. Hematol., 2010;38(2):154-62.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  15. Expansion of CD133-expressing liver cancer stem cells in liver-specific phosphatase and tensin homolog deleted on chromosome 10-deleted mice.
    Authors: Rountree CB, Ding W, He L, Stiles B
    Stem Cells, 2009;27(2):290-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  16. Wnt/beta-catenin/CBP signaling maintains long-term murine embryonic stem cell pluripotency.
    Authors: Miyabayashi T, Teo JL, Yamamoto M, McMillan M, Nguyen C, Kahn M
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(13):5668-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
  17. Induction of pluripotent stem cells from adult human fibroblasts by defined factors.
    Authors: Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S
    Cell, 2007;131(5):861-72.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Expand to show all 17 Citations
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