Key Product Details

Validated by

Knockout/Knockdown, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Porcine, Bovine, Goat, Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio anubis (Olive Baboon), Transgenic Mouse, Xenograft

Applications

Validated:

Knockout Validated, Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, Simple Western, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry, Differentiation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 189502
View all alpha-Fetoprotein/AFP Primary Antibodies »

Product Specifications

Immunogen

Human umbilical cord serum-derived alpha ‑Fetoprotein/AFP

Specificity

Detects human alpha ‑Fetoprotein/AFP in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse alpha-Fetoprotein/AFP Antibody

Detection of a-Fetoprotein/AFP antibody by Western Blot.

Detection of alpha ‑Fetoprotein/AFP by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
a-Fetoprotein/AFP antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line.

a-Fetoprotein/AFP was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of a-Fetoprotein/AFP antibody in HepG2 Human Cell Line antibody by Flow Cytometry.

Detection of alpha ‑Fetoprotein/AFP in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of Human a-Fetoprotein/AFP antibody by Simple WesternTM.

Detection of Human alpha ‑Fetoprotein/AFP by Simple WesternTM.

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for alpha ‑Fetoprotein/AFP at approximately 70 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human/Mouse alpha ‑Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human a-Fetoprotein/AFP Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human alpha -Fetoprotein/AFP Specificity by Using Knockout Cell Line.

Western blot shows lysates of HepG2 human hepatocellular carcinoma parental cell line and a-Fetoprotein/AFP knockout HepG2 cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Fetoprotein/AFP at approximately 70 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
a-Fetoprotein/AFP Antibody Specificity is Shown by Flow Cytometry antibody in Knockout Cell Line.

alpha -Fetoprotein/AFP Specificity is Shown by Flow Cytometry in Knockout Cell Line.

a-Fetoprotein/AFP knockout HepG2 hepatocellular carcinoma cell line was stained with Mouse Anti-Human a-Fetoprotein/AFP Monoclonal Antibody (Catalog # MAB1368, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by PE-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0102B). No staining in the a-Fetoprotein/AFP knockout HepG2 cell line was observed. View our protocol for Staining Membrane-associated Proteins.
Detection of Human alpha-Fetoprotein/AFP by Immunocytochemistry/Immunofluorescence

Detection of Human alpha-Fetoprotein/AFP by Immunocytochemistry/Immunofluorescence

Functional glycosylation of alpha ‐dystroglycan and characterization of dystroglycanopathy patient‐specific iPSCsCurrent model of the core M3 functional glycan structure on alpha ‐dystroglycan and enzymes involved in its synthesis. ECM ligands, such as laminins, bind to the Xyl‐GlucA disaccharide repeats (IIH6 epitope). Man, mannose; GlcNAc, N‐acetylglucosamine; GalNAc, N‐acetylgalactosamine; Rbo5P, ribitol‐5‐phosphate; Xyl, xylose; GlcA, glucuronic acid.Representative images of immunostaining demonstrate that FKRP‐iPSCs express specific pluripotency‐associated markers, including NANOG, OCT4, Tra‐1‐60, and SSEA4.FKRP‐iPSCs have a normal karyotype.In vitro differentiation of FKRP‐iPSCs to cells representing ectoderm ( beta ‐III Tubulin, Tuj1), mesoderm (SMA, smooth muscle actin), and endoderm (AFP, alpha ‐fetoprotein).Data information: Scale bars, 50 μm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31566294), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human alpha-Fetoprotein/AFP by Immunocytochemistry/Immunofluorescence

Detection of Human alpha-Fetoprotein/AFP by Immunocytochemistry/Immunofluorescence

Verification of the pluripotency of the iPSC lines with the TDP-43 A90V mutation.(A) Fluorescence microscopy images of the expression of the pluripotency markers NANOG, OCT4, SSEA4, TRA-1-60, and TRA-1-81 in control (37L25) and patient (36L10) iPSC lines. Scale bar: 20 µm. (B) All iPSC lines differentiated into cells of the three germ layers, as shown by expression of desmin (mesoderm), TUJ1 (ectoderm), and alpha-fetoprotein (AFP, endoderm). These analyses indicate iPSC lines generated here are indeed pluripotent. Scale bar: 20 µm. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0076055), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse alpha-Fetoprotein/AFP by Immunohistochemistry

Detection of Mouse alpha-Fetoprotein/AFP by Immunohistochemistry

Unchanged tumor marker levels in liver, lung, brain, and skin of plasma-treated mice. Representative images of immunohistochemistry of different tumor markers (TM): AFP (I) in liver, beta 2M in brain (II), CEA in lung (III), as well as NSE staining in skin tissue (IV) after one year in positive control (+ve ctrl, left) and plasma-treated (right) animals (A). Scale bar 50 µm (right columns) or 100 µm (left columns). Using ELISA, we analyzed AFP (I), and calcitonin (CT) (II), a TM of medullary thyroid carcinoma, in blood serum (B; * p < 0.05; (n > 9). The mRNA expression levels of five TM and beta -actin in liver, brain, lung, and ear skin tissues from plasma- and untreated (ctrl) mice were compared with organs from a HCC-bearing mouse (+ve ctrl). At least three independent experiments were performed and summarized in the indicated experimental groups (m, male; f, female). AFP, alpha -fetoprotein; NOPE, neighbor of Punc 11; beta 2M, beta 2-microglobulin; NSE, neuron specific enolase; CEA, carcinoembryonic antigen (C). Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/18/4/868), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Human alpha-Fetoprotein/AFP by Immunocytochemistry/Immunofluorescence

Detection of Human alpha-Fetoprotein/AFP by Immunocytochemistry/Immunofluorescence

Pertussis toxin does not affect the maintenance of pluripotency pluripotent stem cell colonies or the ability to form all three embryonic germ layers.(A) Pertussis toxin does not affect the maintenance of pluripotency of hES or hiPS cells as assessed by the expression of the pluripotency markers Nanog, Oct4, Sox2, TDGF1, and ZFP42 by quantitative real-time PCR. (B) Immunocytochemistry of TRA-1-81 (green) and Oct-3/4 (red) also did not reveal differences between hES or hiPS cells treated with pertussis toxin and control treatments in pluripotent stem cell colonies. Nuclei were stained with Hoechst (blue). Scale bar, 200 µm. (C) hES or iPS cells treated with pertussis toxin formed embryoid bodies and differentiated into cell types of the all three embryonic germ layers as assessed by immunocytochemistry for alpha -fetoprotein (a marker of endoderm), alpha -smooth muscle actin (mesoderm) and beta III-tubulin (ectoderm). Scale bar, 100 µm. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/19936228), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse alpha-Fetoprotein/AFP Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line and NCTC 1469 mouse liver cell line

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 human hepatocellular carcinoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Knockout Validated

alpha ‑Fetoprotein/AFP is specifically detected in HepG2 human hepatocellular carcinoma parental cell line but is not detectable in alpha ‑Fetoprotein/AFP knockout HepG2 cell line.

Simple Western

5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Western Blot

0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 12 reviews rated 4.1 using MAB1368 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: alpha-Fetoprotein/AFP

alpha -Fetoprotein is a major plasma protein in the fetus. Its concentration is normally low in the adult except when produced by certain tumors. AFP is produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and billirubin and can found in mono-, di or trimeric forms.

Alternate Names

AFP, DSCAM2

Entrez Gene IDs

174 (Human); 11576 (Mouse); 24177 (Rat)

Gene Symbol

AFP

Additional alpha-Fetoprotein/AFP Products

Product Documents for Human/Mouse alpha-Fetoprotein/AFP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse alpha-Fetoprotein/AFP Antibody

For research use only

Citations for Human/Mouse alpha-Fetoprotein/AFP Antibody

Customer Reviews for Human/Mouse alpha-Fetoprotein/AFP Antibody (12)

4.1 out of 5
12 Customer Ratings
5 Stars
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4 Stars
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3 Stars
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2 Stars
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Showing  1 - 5 of 12 reviews Showing All
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  • Human/Mouse alpha-Fetoprotein/AFP Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: cerebral organoid
    Species: Human
    Verified Customer | Posted 05/20/2023
    1:500 dilution is good
    Human/Mouse alpha-Fetoprotein/AFP Antibody MAB1368
  • Human/Mouse alpha-Fetoprotein/AFP Antibody
    Name: Ricardo Malvicini
    Application: Flow Cytometry
    Sample Tested: HepG2 human hepatocellular carcinoma cell line
    Species: Human
    Verified Customer | Posted 03/29/2022
    Human/Mouse alpha-Fetoprotein/AFP Antibody MAB1368
  • Human/Mouse alpha-Fetoprotein/AFP Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: hepatic cells
    Species: Mouse
    Verified Customer | Posted 10/03/2021
    Human/Mouse alpha-Fetoprotein/AFP Antibody MAB1368
  • Human/Mouse alpha-Fetoprotein/AFP Antibody
    Name: Ricardo Malvicini
    Application: Western Blot
    Sample Tested: Cell Lysates
    Species: Mouse
    Verified Customer | Posted 09/10/2021
    Human/Mouse alpha-Fetoprotein/AFP Antibody MAB1368
  • Human/Mouse alpha-Fetoprotein/AFP Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: hepatocellular carcinoma
    Species: Human
    Verified Customer | Posted 08/12/2021
    Human/Mouse alpha-Fetoprotein/AFP Antibody MAB1368
  • Human/Mouse alpha-Fetoprotein/AFP Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 06/10/2020
    Human/Mouse alpha-Fetoprotein/AFP Antibody MAB1368
  • Human/Mouse alpha -Fetoprotein/AFP Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/20/2018
  • Human/Mouse alpha -Fetoprotein/AFP Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human/Mouse alpha -Fetoprotein/AFP Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma and Serum
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human/Mouse alpha -Fetoprotein/AFP Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: pig trabecular meshwork
    Species: Pig
    Verified Customer | Posted 12/05/2016
    Detection of AFP in cultured pig trabecular meshwork cells using Human/Mouse alpha-Fetoprotein/AFP Antibody (MAB1368) at 1:500 dilution overnight and donkey anti-mouse Alexa 594 (1:1000) for 1 hour.
    Human/Mouse alpha-Fetoprotein/AFP Antibody MAB1368
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 23658023
    Species: Human
    Verified Customer | Posted 02/04/2015
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 22829291
    Species: Human
    Verified Customer | Posted 02/04/2015

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