Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry, Immunocytochemistry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Polyclonal Goat IgG
View all Brachyury Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Brachyury
Ser2-Glu202
Accession # O15178

Specificity

Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse Brachyury APC‑conjugated Antibody

Detection of Brachyury antibody in D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry.

D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085A, filled histogram) or isotype control antibody (IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Detection of Brachyury in IPSC mesoderm differentiation cells by Flow Cytometry.

IPSC mesoderm differentiation cells treated with IPSC mesoderm differentiation (SC030B) for 2 days were stained with Goat Anti-Human/Mouse Brachyury APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with FC004 and permeabilized with FoxP3 Perm. View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse Brachyury APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005) and IPSC mesoderm differentiation cells treated with IPSC mesoderm differentiation (Catalog # SC030B) for 2 days.

Reviewed Applications

Read 1 review rated 5 using IC2085A in the following applications:

Spectra Viewer

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Brachyury

Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.

Alternate Names

brachyury protein, MGC104817, Protein T, SAVA, T brachyury (mouse) homolog, T brachyury homolog, T Brachyury Transcription Factor, T, brachyury homolog (mouse), T-Box Transcription Factor T, TBXT, TF, TFT

Entrez Gene IDs

6862 (Human)

Gene Symbol

TBXT

UniProt

O15178

Additional Brachyury Products

Product Documents for Human/Mouse Brachyury APC‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse Brachyury APC‑conjugated Antibody

For research use only

Citations for Human/Mouse Brachyury APC‑conjugated Antibody

Customer Reviews for Human/Mouse Brachyury APC‑conjugated Antibody (1)

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  • Human/Mouse Brachyury APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human induced pluripotent stem cell derived mesoderm cells
    Species: Human
    Verified Customer | Posted 09/22/2016
    Human induced pluripotent stem cells were differentiated into mesodermal lineage cells for 4 days in culture. Cells were fixed, permeabilized, and stained with or without antibody for 45 minutes. Unstained and Brachyury-APC stained samples were run on a BD Accuri C6 flow cytometer for analysis.
    Human/Mouse Brachyury APC‑conjugated Antibody IC2085A

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