Detects human Brachyury in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) TBX-6 is observed, and approximately 5% cross-reactivity with rhTBX-2, rhTBX-5, and rhTBX-18 is observed.
Polyclonal Goat IgG
E. coli-derived recombinant human Brachyury Ser2-Glu202 Accession # O15178
Supplied in a saline solution containing BSA and Sodium Azide.
Detection of Brachyury in D3 Mouse Cell Line by Flow Cytometry.
D3 mouse embryonic stem cell line differentiated with mouse serum for 4 days was stained with Goat Anti-Human/Mouse Brachyury APC‑conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # IC2085A, filled histogram) or isotype control antibody (Catalog # IC108A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Brachyury is the founding member of the T-box family of transcription factors, which is characterized by the N-terminal conserved DNA-binding T-domain. Brachyury is required in the early determination and differentiation of mesoderms. Human brachyury molecule shares 90% homology with mouse brachyury.
Entrez Gene IDs:
brachyury protein; Brachyury; MGC104817; Protein T; T brachyury (mouse) homolog; T brachyury homolog; T, brachyury homolog (mouse); TF; TFT
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Human induced pluripotent stem cell derived mesoderm cells
Other Experimental Details
Other Experimental Details
Human induced pluripotent stem cells were differentiated into mesodermal lineage cells for 4 days in culture. Cells were fixed, permeabilized, and stained with or without antibody for 45 minutes. Unstained and Brachyury-APC stained samples were run on a BD Accuri C6 flow cytometer for analysis.