Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 173317
View all Cathepsin B Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin B
His18-Phe339
Accession # P10605

Specificity

Detects the pro and active forms of human and mouse Cathepsin B in direct ELISAs and Western blots. Does not cross‑react with recombinant human Cathepsin C, L, L2, O, S, Z, recombinant mouse Cathepsin C, H, L, or Z.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Human/Mouse Cathepsin B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Cathepsin B (Catalog # 953-CY)
Recombinant Mouse Cathepsin B (Catalog # 965-CY)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cathepsin B

Cathepsin B is a cysteine protease that is known to process a number of proteins. It is up-regulated or redistributed in human and animal tumors.

Alternate Names

CTSB

Entrez Gene IDs

1508 (Human); 13030 (Mouse)

Gene Symbol

CTSB

UniProt

P10605

Additional Cathepsin B Products

Product Documents for Human/Mouse Cathepsin B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse Cathepsin B Antibody

For research use only

Citations for Human/Mouse Cathepsin B Antibody

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FAQs

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