Home / Cathepsin B / Human/Mouse Cathepsin B Antibody

Human/Mouse Cathepsin B Antibody

Catalog #: MAB965
3 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB965
MAB965-SP
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews
Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse Cathepsin B Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects the pro and active forms of human and mouse Cathepsin B in direct ELISAs and Western blots. Does not cross‑react with recombinant human Cathepsin C, L, L2, O, S, Z, recombinant mouse Cathepsin C, H, L, or Z.
Source
Monoclonal Rat IgG1 Clone # 173317
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin B
His18-Phe339
Accession # P10605
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Cathepsin B (Catalog # 953-CY)
Recombinant Mouse Cathepsin B (Catalog # 965-CY)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin B

Cathepsin B is a cysteine protease that is known to process a number of proteins. It is up-regulated or redistributed in human and animal tumors.

Entrez Gene IDs
1508 (Human); 13030 (Mouse)
Alternate Names
APP secretase; APPS; Cathepsin B; Cathepsin B1; CPSBamyloid precursor protein secretase; CTSB; cysteine protease; EC 3.4.22; EC 3.4.22.1

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Citations for Human/Mouse Cathepsin B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. C9orf72 and smcr8 mutant mice reveal MTORC1 activation due to impaired lysosomal degradation and exocytosis
    Authors: Q Shao, M Yang, C Liang, L Ma, W Zhang, Z Jiang, J Luo, JK Lee, C Liang, JF Chen
    Autophagy, 2019;0(0):1-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Induction of cathepsin B by the CXCR3 chemokines CXCL9 and CXCL10 in human breast cancer cells
    Authors: H Bronger, A Karge, T Dreyer, D Zech, S Kraeft, S Avril, M Kiechle, M Schmitt
    Oncol Lett, 2017;13(6):4224-4230.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Intra- versus extracellular effects of microglia-derived cysteine proteases in a conditioned medium transfer model.
    Authors: Wendt W, Schulten R, Stichel CC, Lubbert H
    J. Neurochem., 2009;110(6):1931-41.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

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Isotype Controls

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Reconstitution Buffers

Reconstitution Buffer 1 (PBS)

RB01
3 Reviews

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