Human/Mouse CXCL12/SDF-1 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
IC350A
Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry.
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Product Details
Citations (8)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse CXCL12/SDF-1 APC-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 79018
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of CXCL12/SDF-1 antibody in MG-63 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 APC-conjugated Mono-clonal Antibody (Catalog # IC350A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL12/SDF-1

CXCL12, also known as SDF-1, is a CXC chemokine that interacts with CXCR4. SDF-1 alpha and SDF-1 beta are alternatively spliced isoforms of CXCL12. SDF-1 beta has an additional 4 amino acids at the carboxy-terminus.

Entrez Gene IDs
6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)
Alternate Names
chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1

Product Datasheets

Citations for Human/Mouse CXCL12/SDF-1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Follicular helper T cells recruit eosinophils into host liver by producing CXCL12 during Schistosoma japonicum infection
    Authors: X Chen, Z Xu, C Wei, X Yang, L Xu, S Zhou, J Zhu, C Su
    J. Cell. Mol. Med., 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Multiple myeloma exploits Jagged1 and Jagged2 to promote intrinsic and bone marrow-dependent drug resistance
    Authors: M Colombo, S Garavelli, M Mazzola, N Platonova, D Giannandre, R Colella, L Apicella, M Lancellott, E Lesma, S Ancona, MT Palano, M Barbieri, E Taiana, E Lazzari, A Basile, M Turrini, A Pistocchi, A Neri, R Chiaramont
    Haematologica, 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Distinct Bone Marrow Sources of Pleiotrophin Control Hematopoietic Stem Cell Maintenance and Regeneration
    Authors: HA Himburg, CM Termini, L Schlussel, J Kan, M Li, L Zhao, T Fang, JP Sasine, VY Chang, JP Chute
    Cell Stem Cell, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Differential cytokine contributions of perivascular haematopoietic stem cell niches
    Authors: N Asada, Y Kunisaki, H Pierce, Z Wang, NF Fernandez, A Birbrair, A Ma'ayan, PS Frenette
    Nat. Cell Biol, 2017;19(3):214-223.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Downregulation of CXCL12 in mesenchymal stromal cells by TGF? promotes breast cancer metastasis
    Oncogene, 2016;0(0):.
  6. Inflamm-aging influences immune cell survival factors in human bone marrow
    Eur. J. Immunol, 2016;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. DOCK2 is critical for CD8(+) TCR(-) graft facilitating cells to enhance engraftment of hematopoietic stem and progenitor cells.
    Authors: Wen Y, Elliott M, Huang Y, Miller T, Corbin D, Hussain L, Ratajczak M, Fukui Y, Ildstad S
    Stem Cells, 2014;32(10):2732-43.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. The Spiegelmer NOX-A12, a novel CXCL12 inhibitor, interferes with chronic lymphocytic leukemia cell motility and causes chemosensitization.
    Authors: Hoellenriegel J, Zboralski D, Maasch C, Rosin N, Wierda W, Keating M, Kruschinski A, Burger J
    Blood, 2014;123(7):1032-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human/Mouse CXCL12/SDF-1 APC-conjugated Antibody

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Human/Mouse CXCL12/SDF-1 APC-conjugated Antibody
By Kihong Lim on 09/29/2016
Application: Flow Cytometry Sample Tested: Bone marrow cells,Mouse spleenocytes Species: Mouse