Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 79018
View all CXCL12/SDF-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0

Specificity

Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse CXCL12/SDF‑1 APC‑conjugated Antibody

Detection of CXCL12/SDF-1 antibody in MG-63 Human Cell Line antibody by Flow Cytometry.

Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry.

MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 APC-conjugated Mono-clonal Antibody (Catalog # IC350A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse CXCL12/SDF‑1 APC‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: MG‑63 human osteosarcoma cell line fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Reviewed Applications

Read 1 review rated 5 using IC350A in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL12/SDF-1

CXCL12, also known as SDF-1, is a CXC chemokine that interacts with CXCR4. SDF-1 alpha and SDF-1 beta are alternatively spliced isoforms of CXCL12. SDF-1 beta has an additional 4 amino acids at the carboxy-terminus.

Alternate Names

PBSF, SDF-1, SDF1

Entrez Gene IDs

6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)

Gene Symbol

CXCL12

UniProt

Q6ICW0

Additional CXCL12/SDF-1 Products

Product Documents for Human/Mouse CXCL12/SDF‑1 APC‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse CXCL12/SDF‑1 APC‑conjugated Antibody

For research use only

Citations for Human/Mouse CXCL12/SDF‑1 APC‑conjugated Antibody

Customer Reviews for Human/Mouse CXCL12/SDF‑1 APC‑conjugated Antibody (1)

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Customer Images

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  • Human/Mouse CXCL12/SDF-1 APC-conjugated Antibody
    Name: Kihong Lim
    Application: Flow Cytometry
    Sample Tested: Bone marrow cells and Mouse spleenocytes
    Species: Mouse
    Verified Customer | Posted 09/29/2016
    Human/Mouse CXCL12/SDF‑1 APC‑conjugated Antibody IC350A

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