Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody

Catalog # Availability Size / Price Qty
Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry.
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Product Details
Citations (2)
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Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse CXCL12/SDF-1 alpha and CXCL12/SDF-1 beta in ELISAs.
Monoclonal Mouse IgG1 Clone # 79018
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Accession # Q6ICW0
Supplied in a saline solution containing BSA and Sodium Azide.
Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

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Recommended Concentration
Intracellular Staining by Flow Cytometry
Please refer to the PDF datasheet for specifications.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CXCL12/SDF-1 antibody in MG-63 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of CXCL12/SDF‑1 in MG‑63 Human Cell Line by Flow Cytometry. MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Mono-clonal Antibody (Catalog # IC350F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CXCL12/SDF-1

CXCL12, also known as SDF-1, is a CXC chemokine that interacts with CXCR4. SDF-1 alpha and SDF-1 beta are alternatively spliced isoforms of CXCL12. SDF-1 beta has an additional 4 amino acids at the carboxy-terminus.

Entrez Gene IDs
6387 (Human); 20315 (Mouse); 493806 (Feline); 574334 (Rhesus Macaque)
Alternate Names
chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; CXCL12; hIRH; hSDF-1; Intercrine reduced in hepatomas; IRH; PBSF; PBSFSDF-1a; Pre-B cell growth-stimulating factor; SCYB12; SDF1; SDF-1; SDF1A; SDF1B; SDF1SDF-1b; stromal cell-derived factor 1; TLSF; TLSF-a; TLSF-b; TPAR1

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Citations for Human/Mouse CXCL12/SDF-1 Fluorescein-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. CXCR4 inhibition in human pancreatic and colorectal cancers induces an integrated immune response
    Authors: D Biasci, M Smoragiewi, CM Connell, Z Wang, Y Gao, JED Thaventhir, B Basu, L Magiera, TI Johnson, L Bax, A Gopinathan, C Isherwood, FA Gallagher, M Pawula, I Hudecova, D Gale, N Rosenfeld, P Barmpounak, EC Popa, R Brais, E Godfrey, F Mir, FM Richards, DT Fearon, T Janowitz, DI Jodrell
    Proc Natl Acad Sci U S A, 2020;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  2. Long-term B cell depletion in murine lupus eliminates autoantibody-secreting cells and is associated with alterations in the kidney plasma cell niche.
    Authors: Wang, Wensheng, Rangel-Moreno, Javier, Owen, Teresa, Barnard, Jennifer, Nevarez, Sarah, Ichikawa, H Travis, Anolik, Jennifer
    J Immunol, 2014;192(7):3011-20.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr


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