Human/Mouse EMX2 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF6470N Datasheet / COA / SDS

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AF6470N-100UG

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Human/Mouse EMX2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human EMX2 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human EMX2
Asn49-Ser143
Accession # Q04743

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: EMX2

EMX-2 (also Empty spiracles homolog 2) is a 30-33 kDa member of the EMX homeobox family of molecules. It is expressed in embryonic neural stem and progenitor cells, and promotes their commitment to a neuronal phenotype. In a more global sense, it also promotes the formation of networks that favor visual sensation. In the adult, EMX-2 is found in neural stem cells where it acts to maintain a pool of available stem cells. Human EMX-2 is 252 amino acids (aa) in length. It contains one poly-Ala region (aa 54-59) and a DNA-binding homeobox domain (aa 154-213). There is one potential splice variant that shows a 33 aa substitution for aa 137-252. Over aa 59-143, human EMX-2 shares 98% aa identity with mouse EMX-2.

Long Name

Empty Spiracles Homeobox 2

Entrez Gene IDs

2018 (Human); 13797 (Mouse); 499380 (Rat)

Alternate Names

empty spiracles homeobox 2; empty spiracles homolog 2 (Drosophila); Empty spiracles homolog 2; Empty spiracles-like protein 2; EMX2; homeobox protein EMX2