Human/Mouse EMX2 Antibody Summary
Accession # Q04743
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Mouse EMX2 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Human EMX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6470) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMX2 at approximately 33 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
EMX-2 (also Empty spiracles homolog 2) is a 30-33 kDa member of the EMX homeobox family of molecules. It is expressed in embryonic neural stem and progenitor cells, and promotes their commitment to a neuronal phenotype. In a more global sense, it also promotes the formation of networks that favor visual sensation. In the adult, EMX-2 is found in neural stem cells where it acts to maintain a pool of available stem cells. Human EMX-2 is 252 amino acids (aa) in length. It contains one poly-Ala region (aa 54-59) and a DNA-binding homeobox domain (aa 154-213). There is one potential splice variant that shows a 33 aa substitution for aa 137-252. Over aa 59-143, human EMX-2 shares 98% aa identity with mouse EMX-2.
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