Human/Mouse FoxP2 Antibody

Catalog # Availability Size / Price Qty
AF5647
AF5647-SP

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Detection of Human FoxP2 by Western Blot.
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Product Details
Citations (28)
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Human/Mouse FoxP2 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse FoxP2 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human FoxP2 isoform 1
Ala640-Glu715
Accession # O15409
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human FoxP2 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A172 human glioblastoma cell line and U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for FoxP2 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Immunocytochemistry FoxP2 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

FoxP2 in HeLa Human Cell Line. FoxP2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Sheep Anti-Human/Mouse FoxP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5647) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FoxP2

FoxP2 (Forkhead box protein P2; also CAGH44) is an 85‑95 kDa member of the winged helix transcription factor gene family. It is a transcriptional repressor that is expressed principally in gut and lung epithelium, and in neurons involved in speech and language. Human FoxP2 is 715 amino acids (aa) in length. It contains a huge poly-Gln region (aa 123‑231), a C2H2 Zn-finger domain (aa 346‑371), a Leu-zipper segment (aa 388‑409) and a forkhead DNA binding domain (aa 504‑594). FoxP2 both homodimerizes and heterodimerizes with FoxP1 and P4, and binds CTBP1. There are multiple splice variants. There is an alternate start site at Met93, an 11 aa substitution for aa 57‑715, a 25 aa insert after Gln86, an 11 aa substitution for aa 133‑715, and a ten aa substitution for aa 423‑715. Over aa 587‑715, human and mouse FoxP2 are (100%) identical in aa sequence.

Long Name
Forkhead Box P2
Entrez Gene IDs
93986 (Human); 114142 (Mouse); 500037 (Rat)
Alternate Names
CAG repeat protein 44; CAGH44; CAGH44TNRC10forkhead box protein P2; DKFZp686H1726; forkhead box P2; forkhead/winged-helix transcription factor; FoxP2; SPCH1; TNRC10; trinucleotide repeat containing 10; Trinucleotide repeat-containing gene 10 protein

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Citations for Human/Mouse FoxP2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. Efferent projections of Nps -expressing neurons in the parabrachial region
    Authors: Richie Zhang, Dake Huang, Silvia Gasparini, Joel C. Geerling
    bioRxiv
  2. Opioid suppression of an excitatory pontomedullary respiratory circuit by convergent mechanisms
    Authors: Jordan T Bateman, Erica S Levitt
    eLife
  3. Lateral parabrachial FoxP2 neurons regulate respiratory responses to hypercapnia
    Authors: Kaur, S;Nicole, L;Sela, Y;Lima, J;Thomas, R;Bandaru, S;Saper, C;
    Research square
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Cerebellar nuclei neurons projecting to the lateral parabrachial nucleus modulate classical fear conditioning
    Authors: KD Hwang, J Baek, HH Ryu, J Lee, HG Shim, SY Kim, SJ Kim, YS Lee
    Cell Reports, 2023-03-22;42(4):112291.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Neuropeptide S (NPS) neurons: Parabrachial identity and novel distributions
    Authors: Dake Huang, Richie Zhang, Silvia Gasparini, Miriam C. McDonough, William J. Paradee, Joel C. Geerling
    Journal of Comparative Neurology
  6. GIPR Agonism Inhibits PYY-Induced Nausea-Like Behavior
    Authors: Ricardo J. Samms, Richard Cosgrove, Brandy M. Snider, Ellen C. Furber, Brian A. Droz, Daniel A. Briere et al.
    Diabetes
  7. Molecular ontology of the parabrachial nucleus
    Authors: Samyukta Karthik, Dake Huang, Yaritza Delgado, Justin J. Laing, Lila Peltekian, Gabrielle N. Iverson et al.
    Journal of Comparative Neurology
  8. Efferent projections of CGRP/Calca‐expressing parabrachial neurons in mice
    Authors: Dake Huang, Fillan S. Grady, Lila Peltekian, Justin J. Laing, Joel C. Geerling
    Journal of Comparative Neurology
  9. Midbrain dopaminergic inputs gate amygdala intercalated cell clusters by distinct and cooperative mechanisms in male mice
    Authors: Ayla Aksoy-Aksel, Andrea Gall, Anna Seewald, Francesco Ferraguti, Ingrid Ehrlich
    eLife
  10. Efferent projections of Vglut2, Foxp2, and Pdyn parabrachial neurons in mice
    Authors: Dake Huang, Fillan S. Grady, Lila Peltekian, Joel C. Geerling
    Journal of Comparative Neurology
  11. Input–output connections of LJA5 prodynorphin neurons
    Authors: Lindsay J. Agostinelli, Madison R. Mix, Marco M. Hefti, Thomas E. Scammell, Alexander G. Bassuk
    Journal of Comparative Neurology
  12. Phox2a Defines a Developmental Origin of the Anterolateral System in Mice and Humans
    Authors: RB Roome, FB Bourojeni, B Mona, S Rastegar-P, R Blain, A Dumouchel, C Salesse, WS Thompson, M Brookbank, Y Gitton, L Tessarollo, M Goulding, JE Johnson, M Kmita, A Chédotal, A Kania
    Cell Rep, 2020-11-24;33(8):108425.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  13. The cellular basis of distinct thirst modalities
    Authors: AH Pool, T Wang, DA Stafford, RK Chance, S Lee, J Ngai, Y Oka
    Nature, 2020-10-14;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Identifying Parabrachial Neurons Selectively Regulating Satiety for Highly Palatable Food in Mice
    Authors: Erica Rodriguez, David Ryu, Shengli Zhao, Bao-Xia Han, Fan Wang
    eNeuro
  15. Chemosensory modulation of neural circuits for sodium appetite
    Authors: S Lee, V Augustine, Y Zhao, H Ebisu, B Ho, D Kong, Y Oka
    Nature, 2019-03-27;568(7750):93-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Aldosterone-sensitive HSD2 neurons in mice
    Authors: Silvia Gasparini, Jon M. Resch, Sowmya V. Narayan, Lila Peltekian, Gabrielle N. Iverson, Samyukta Karthik et al.
    Brain Structure and Function
  17. Challenges to Body Fluid Homeostasis Differentially Recruit Phasic Dopamine Signaling in a Taste-Selective Manner
    Authors: Samantha M. Fortin, Mitchell F. Roitman
    The Journal of Neuroscience
  18. Efferent projections of excitatory and inhibitory preB�tzinger complex neurons
    Authors: CF Yang, JL Feldman
    J. Comp. Neurol., 2018-03-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Expression of the transcription factor FOXP2 in brainstem respiratory circuits of adult rat is restricted to upper-airway pre-motor areas
    Authors: D Stani?, RR Dhingra, M Dutschmann
    Respir Physiol Neurobiol, 2018-02-04;250(0):14-18.
    Species: Rat, Western Blot
    Sample Types: Cell Lysates, Whole Tissues
    Applications: IHC, Western Blot
  20. Barrington's nucleus: Neuroanatomic landscape of the mouse pontine micturition center
    Authors: AM Verstegen, V Vanderhors, PA Gray, ML Zeidel, JC Geerling
    J. Comp. Neurol, 2017-04-18;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. K�lliker-Fuse GABAergic and glutamatergic neurons project to distinct targets
    Authors: Joel Geerling
    J. Comp. Neurol, 2017-03-14;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  22. HSD2 neurons in the hindbrain drive sodium appetite
    Nat. Neurosci, 2016-12-05;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Blockade of ENaCs by amiloride induces c-Fos activation of the area postrema
    Authors: Rebecca L. Miller, George O. Denny, Mark M. Knuepfer, Thomas R. Kleyman, Edwin K. Jackson, Lawrence B. Salkoff et al.
    Brain Research
  24. Fos-activation of FoxP2 and Lmx1b neurons in the parabrachial nucleus evoked by hypotension and hypertension in conscious rats
    Authors: Rebecca L. Miller, Mark M. Knuepfer, Michelle H. Wang, George O. Denny, Paul A. Gray, Arthur D. Loewy
    Neuroscience
  25. Serotonergic Inputs to the FoxP2 neurons of the Pre-Locus Coeruleus and Parabrachial Nuclei That Project to the Ventral Tegmental Area
    Authors: Rebecca L. Miller, Matthew K. Stein, Arthur D. Loewy
    Neuroscience
  26. FoxP2 brainstem neurons project to sodium appetite regulatory sites
    Authors: Jung-Won Shin, Joel C. Geerling, Matthew K. Stein, Rebecca L. Miller, Arthur D. Loewy
    Journal of Chemical Neuroanatomy
  27. FoxP2 expression defines dorsolateral pontine neurons activated by sodium deprivation*
    Authors: Joel C. Geerling, Matthew K. Stein, Rebecca L. Miller, Jung-Won Shin, Paul A. Gray, Arthur D. Loewy
    Brain Research
  28. Area postrema projects to FoxP2 neurons of the pre-locus coeruleus and parabrachial nuclei: Brainstem sites implicated in sodium appetite regulation
    Authors: Matthew K. Stein, Arthur D. Loewy
    Brain Research

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