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OR

Human/Mouse FoxP3 Alexa Fluor® 488‑conjugated Antibody

  • Specificity
    Detects human and mouse FoxP3 in flow cytometry.
  • Source
    Monoclonal Rabbit IgG Clone # 1054C
  • Purification
    Protein A or G purified from cell culture supernatant
  • Immunogen
    E. coli-derived recombinant human FoxP3
    Met1-Leu71
    Accession # Q9BZS1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of FoxP3 in Human PBMC Natural Tregs by Flow Cytometry. Human peripheral blood mononuclear cell (PBMC) natural regulatory T cells (Tregs) were surface stained with (A) Mouse Anti-Human CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB3791P) and (B) Mouse Anti-Human IL-2 Ra (CD25) APC-conjugated Monoclonal Antibody (Catalog # FAB1020A), followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC8214G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.
Intracellular Staining by Flow Cytometry
Detection of FoxP3 in C57BL/6 Mouse Splenocyte Natural Tregs by Flow Cytometry. C57BL/6 mouse splenocyte natural regulatory T cells (Tregs) were surface stained with (A) Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and (B) Rat Anti-Mouse IL-2 Ra (CD25) APC-conjugated Monoclonal Antibody (Catalog # FAB2438A) followed by intracellular staining using Rabbit Anti-Human/Mouse FoxP3 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # IC8214G). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). Cells were gated on lymphocytes.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: FoxP3
Human FoxP3 is a 47 kDa member of the P subclass of the FOX (forkhead box) family of transcription factors. It contains a Leu-rich repeat, a C2H2 zinc finger region, and a C-terminal FKH (fork head), DNA-binding domain. Three isoforms for FoxP3 have been reported. All three isoforms share the sequence used as the immunogen. FoxP3 directly associates with NFAT and NFkB, suppressing their activity in CD4+ T cells. In human, FoxP3 is found in CD4+, CD8+ and CD4+CD25+ T cells. Over the region used for immunization of the amino acid sequence, mouse FoxP3 is 83% to 88% identical to rat, human, canine, and bovine FoxP3.
  • Long Name:
    Forkhead Box P3
  • Entrez Gene IDs:
    50943 (Human); 20371 (Mouse);
  • Alternate Names:
    AIID; DIETER; IPEX; JM2; PIDX; SCURFIN; XPID; AIIDMGC141961; forkhead box P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; JM2; PIDXMGC141963; scurfin; Scurfin; SCURFIN; XPIDpolyendocrinopathy, enteropathy, X-linked
Related Research Areas

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