Human/Mouse Frizzled‑7 APC‑conjugated Antibody

R&D Systems | Catalog # FAB1981A

R&D Systems
Discontinued Product
FAB1981A has been discontinued. View all Frizzled-7 products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 151143
View all Frizzled-7 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-7
Gln33-Leu185
Accession # Q61090

Specificity

Detects human and mouse Frizzled-7. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -3, -4 or -8 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human/Mouse Frizzled‑7 APC‑conjugated Antibody

Detection of Frizzled-7 antibody in HEK293 Human Cell Line antibody by Flow Cytometry.

Detection of Frizzled‑7 in HEK293 Human Cell Line by Flow Cytometry.

HEK293 human embryonic kidney cell line was stained with Rat Anti-Human/Mouse Frizzled-7 APC-conjugated Monoclonal Antibody (Catalog # FAB1981A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of Frizzled-7 antibody in RA Treated D3 Mouse Cell Line antibody by Flow Cytometry.

Detection of Frizzled‑7 in RA Treated D3 Mouse Cell Line by Flow Cytometry.

RA treated D3 mouse embryonic stem cell line was stained with Rat Anti-Human/Mouse Frizzled-7 APC-conjugated Monoclonal Antibody (Catalog # FAB1981A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse Frizzled‑7 APC‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: HEK293 human embryonic kidney cell line and RA treated D3 mouse embryonic stem cell line

Reviewed Applications

Read 4 reviews rated 4.5 using FAB1981A in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Frizzled-7

Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1‑3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, Frizzled-7 contains a divergent N‑terminal signal peptide (amino acid (aa) 1‑32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44‑169), a variable-length linker region (aa 170‑256), a seven-pass transmembrane region (aa 257‑549), and a variable-length C-terminal cytoplasmic domain (aa 550‑574) (1‑3). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2, 3). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (4). Within aa 33‑185, human Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta ‑Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 4). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non-canonical signaling pathways (3‑5). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt-7a (3, 6). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (7). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 8). Frizzled-7 mRNA has also been detected in adult heart and placenta (3).

References

  1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
  2. Vincan, E. et al. (2007) Front. Biosci. 12:4558.
  3. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
  4. Medina, A. et al. (2000) Mech. Dev. 92:227.
  5. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
  6. Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
  7. Melchior, K. et al. (2008) Biol. Chem. 389:897.
  8. Vincan, E. et al. (2007) Oncogene 26:2340.

Alternate Names

Frizzled7, FZD7

Entrez Gene IDs

8324 (Human); 14369 (Mouse)

Gene Symbol

FZD7

UniProt

Q61090

Additional Frizzled-7 Products

Product Documents for Human/Mouse Frizzled‑7 APC‑conjugated Antibody

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Product Specific Notices for Human/Mouse Frizzled‑7 APC‑conjugated Antibody

For research use only

Citations for Human/Mouse Frizzled‑7 APC‑conjugated Antibody

Customer Reviews for Human/Mouse Frizzled‑7 APC‑conjugated Antibody (4)

4.5 out of 5
4 Customer Ratings
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Showing  1 - 4 of 4 reviews Showing All
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  • Human/Mouse Frizzled-7 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: limbal stem cell
    Species: Human and Mouse
    Verified Customer | Posted 08/10/2021
    Human/Mouse Frizzled‑7 APC‑conjugated Antibody FAB1981A
  • Human/Mouse Frizzled-7 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human cell line
    Species: Human
    Verified Customer | Posted 02/19/2018
    Human/Mouse Frizzled‑7 APC‑conjugated Antibody FAB1981A
  • Human/Mouse Frizzled-7 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Human cell line
    Species: Human
    Verified Customer | Posted 02/16/2018
    Human/Mouse Frizzled‑7 APC‑conjugated Antibody FAB1981A
  • Name: Gleb Mironov
    Application: Flow Cytometry
    Sample Tested: HEK 293 FZD7-transfected and HEK 293
    Species: Human
    Verified Customer | Posted 04/21/2015
    Pink - positive cells with Ab, Blue - negative cells with Ab, Green - non-stained positive cells, Red - non-stained negative cel
    Human/Mouse Frizzled‑7 APC‑conjugated Antibody FAB1981A

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