Human/Mouse GLI-1 Biotinylated Antibody

Catalog #: BAF3455
1 Citation Datasheet / COA / SDS

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BAF3455

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse GLI-1 Biotinylated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse GLI-1 in Western blot. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) GLI-2 and rmGLI-3 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse GLI-1
Met1-Glu237
Accession # P47806

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human GLI‑1
Recombinant Mouse GLI‑1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-1

GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI-1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.

Long Name

Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]

Entrez Gene IDs

2735 (Human); 14632 (Mouse); 140589 (Rat)

Alternate Names

GLI family zinc finger 1; GLI1; GLI-1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Glioma-associated oncogene; GLIzinc finger protein GLI1; Oncogene GLI

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human/Mouse GLI-1 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A conserved HH-Gli1-Mycn network regulates heart regeneration from newt to human
Authors: BN Singh, N Koyano-Nak, W Gong, IP Moskowitz, CV Weaver, E Braunlin, S Das, JH van Berlo, MG Garry, DJ Garry
Nat Commun, 2018-10-12;9(1):4237.
Species: Mouse
Sample Types: Cell Lysates
Applications: ChIP