Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all GLI-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse GLI-1
Met1-Glu237
Accession # P47806

Specificity

Detects human and mouse GLI-1 in Western blot. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) GLI-2 and rmGLI-3 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse GLI‑1 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human GLI‑1
Recombinant Mouse GLI‑1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: GLI-1

GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI-1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.

Long Name

Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]

Alternate Names

GLI1

Entrez Gene IDs

2735 (Human); 14632 (Mouse); 140589 (Rat)

Gene Symbol

GLI1

UniProt

P47806

Additional GLI-1 Products

Product Documents for Human/Mouse GLI‑1 Biotinylated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse GLI‑1 Biotinylated Antibody

For research use only

Citations for Human/Mouse GLI‑1 Biotinylated Antibody

Customer Reviews for Human/Mouse GLI‑1 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse GLI‑1 Biotinylated Antibody and earn rewards!

Have you used Human/Mouse GLI‑1 Biotinylated Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies