Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Fish

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all GLI-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse GLI-1
Met1-Glu237
Accession # P47806

Specificity

Detects human and mouse GLI-1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse GLI‑1 Antibody

Detection of Human GLI-1 antibody by Western Blot.

Detection of Human GLI‑1 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human GLI-1. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GLI-1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GLI-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

GLI‑1 in HeLa Human Cell Line.

GLI-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of GLI-1 by Western Blot

Detection of GLI-1 by Western Blot

Numb is required for maximal activation of Hh signaling. A Diagram of the Hh signaling cascade. Numb regulates Hh signaling at the level of Ptch1; SmoM2 actives Hh signaling independent of Shh. b, c Hh signaling levels in WT or Numb KO cells were assessed by the transcript levels of the Hh-target genes, Gli1 and Ptch1, via qPCR. d Hh signaling levels in WT cells and Numb KO cells that express Numb-V5 or the blank vector. Hh activity was assessed by Gli1 transcript levels. e, f Western blot analysis and quantification of Gli1 protein levels in WT and Numb KO cells. Prior to harvesting, cells were stimulated with 1 μg/ml Shh in the low serum culture medium for 24 h. g SAG-induced Hh signaling levels in WT cells and Numb KO cells. Prior to harvesting, cells were treated with the indicated doses of SAG in the low serum culture medium for 24 h. h Hh signaling activity in WT cells and Numb KO cells infected with lentiviruses that express SmoM2. All experiments were repeated three to four times with similar results. Data are shown as mean ± SD. Statistics in (b, c, d, f, g, h): Two-way ANOVA with multiple comparisons (Tukey test). ns not significant. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38664376), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse GLI‑1 Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line transfected with human GLI-1

Reviewed Applications

Read 3 reviews rated 4.3 using AF3455 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GLI-1

GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI-1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.

Long Name

Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]

Alternate Names

GLI1

Entrez Gene IDs

2735 (Human); 14632 (Mouse); 140589 (Rat)

Gene Symbol

GLI1

UniProt

P47806

Additional GLI-1 Products

Product Documents for Human/Mouse GLI‑1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse GLI‑1 Antibody

For research use only

Citations for Human/Mouse GLI‑1 Antibody

Customer Reviews for Human/Mouse GLI‑1 Antibody (3)

4.3 out of 5
3 Customer Ratings
5 Stars
33%
4 Stars
67%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • Human/Mouse GLI-1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Brain (cerebral cortex)
    Species: Mouse
    Verified Customer | Posted 01/18/2022
    Used antibody at 20ug/ml overnight at 4C in TBS+1% BSA. In image green=Gli1; blue=DAPI.
    Human/Mouse GLI‑1 Antibody AF3455
  • Human/Mouse GLI-1 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Cell lysate
    Species: Mouse cell line
    Verified Customer | Posted 12/08/2016
  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: See PMID 21698280
    Species: Mouse
    Verified Customer | Posted 01/07/2015

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Protocols

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