Human/Mouse GLI-1 Antibody

Catalog # Availability Size / Price Qty
AF3455
AF3455-SP
Detection of Human GLI‑1 by Western Blot.
2 Images
Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)

Human/Mouse GLI-1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse GLI-1.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GLI-1
Met1-Glu237
Accession # P47806
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human GLI-1 antibody by Western Blot. View Larger

Detection of Human GLI‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human GLI-1. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GLI-1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry GLI-1 antibody in HeLa Human Cell Line by Immunocytochemistry (ICC). View Larger

GLI‑1 in HeLa Human Cell Line. GLI-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GLI-1

GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI-1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.

Long Name
Glioma-Associated Oncogene Homolog 1 [Zinc Finger Protein]
Entrez Gene IDs
2735 (Human); 14632 (Mouse); 140589 (Rat)
Alternate Names
GLI family zinc finger 1; GLI1; GLI-1; glioma-associated oncogene family zinc finger 1; glioma-associated oncogene homolog 1 (zinc finger protein); Glioma-associated oncogene; GLIzinc finger protein GLI1; Oncogene GLI

Product Datasheets

Citations for Human/Mouse GLI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Identification of a novel tumor angiogenesis inhibitor targeting Shh/Gli1 signaling pathway in Non-small cell lung cancer
    Authors: X Lei, Y Zhong, L Huang, S Li, J Fu, L Zhang, Y Zhang, Q Deng, X Yu
    Cell Death Dis, 2020;11(4):232.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  2. Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells
    Authors: A Po, A Citarella, G Catanzaro, ZM Besharat, S Trocchiane, F Gianno, C Sabato, M Moretti, E De Smaele, A Vacca, ME Fiori, E Ferretti
    Sci Rep, 2020;10(1):13988.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells
    Authors: A Po, A Citarella, G Catanzaro, ZM Besharat, S Trocchiane, F Gianno, C Sabato, M Moretti, E De Smaele, A Vacca, ME Fiori, E Ferretti
    Sci Rep, 2020;10(1):13988.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  4. Multicolor quantitative confocal imaging cytometry
    Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
    Nat. Methods, 2018;15(1):39-46.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  5. Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. The hedgehog system machinery controls transforming growth factor-beta-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis.
    Authors: Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Somme J, Wemeau-Stervinou L, Fabre A, Mal H, Leseche G, Dehoux M, Soler P, Crestani B, Mailleux A
    Am J Pathol, 2012;181(6):2126-37.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. A novel synthetic smoothened antagonist transiently inhibits pancreatic adenocarcinoma xenografts in a mouse model.
    Authors: Strand MF, Wilson SR, Dembinski JL, Holsworth DD, Khvat A, Okun I, Petersen D, Krauss S
    PLoS ONE, 2011;6(6):e19904.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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Isotype Controls

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Secondary Antibodies

Reviews for Human/Mouse GLI-1 Antibody

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Human/Mouse GLI-1 Antibody
By Anonymous on 01/18/2022
Application: ICC/IF Sample Tested: Brain (cerebral cortex) Species: Mouse

Used antibody at 20ug/ml overnight at 4C in TBS+1% BSA. In image green=Gli1; blue=DAPI.