Human/Mouse GLI-1 Antibody Summary
Met1-Glu237
Accession # P47806
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human GLI‑1 by Western Blot. Western blot shows lysates of HEK293 human embryonic kidney cell line transfected with human GLI-1. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GLI-1 at approximately 165 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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GLI‑1 in HeLa Human Cell Line. GLI-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using 10 µg/mL Goat Anti-Human/Mouse GLI-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3455) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GLI-1
GLI-1 is a member of the Krupple family of zinc finger proteins. GLI-1 is activated by the sonic hedgehog pathway and influences transcription of a variety of target genes by binding to the consensus site 5’CGGGTGGTC3’. GLI-1 activation leads to cellular proliferation and anti-apoptotic activities based on target genes activated.
Product Datasheets
Citations for Human/Mouse GLI-1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Bone marrow and periosteal skeletal stem/progenitor cells make distinct contributions to bone maintenance and repair
Authors: EC Jeffery, TLA Mann, JA Pool, Z Zhao, SJ Morrison
Cell Stem Cell, 2022-10-21;29(11):1547-1561.e6.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Vaccination-based immunotherapy to target profibrotic cells in liver and lung
Authors: M Sobecki, J Chen, E Krzywinska, S Nagarajan, Z Fan, E Nelius, JM Monné Rodr, F Seehusen, A Hussein, G Moschini, EY Hajam, R Kiran, D Gotthardt, J Debbache, C Badoual, T Sato, T Isagawa, N Takeda, C Tanchot, E Tartour, A Weber, S Werner, J Loffing, L Sommer, V Sexl, C Münz, C Feghali-Bo, E Pachera, O Distler, J Snedeker, C Jamora, C Stockmann
Cell Stem Cell, 2022-09-15;0(0):.
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot -
Dhh signaling pathway regulates reconstruction of seminiferous tubule-like structure
Authors: M Min, T Song, M Sun, T Wang, J Tan, J Zhang
Reproductive biology, 2022-08-17;22(4):100684.
Species: Mouse
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Hedgehog-GLI signalling promotes chemoresistance through the regulation of ABC transporters in colorectal cancer cells
Authors: A Po, A Citarella, G Catanzaro, ZM Besharat, S Trocchiane, F Gianno, C Sabato, M Moretti, E De Smaele, A Vacca, ME Fiori, E Ferretti
Sci Rep, 2020-08-19;10(1):13988.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Identification of a novel tumor angiogenesis inhibitor targeting Shh/Gli1 signaling pathway in Non-small cell lung cancer
Authors: X Lei, Y Zhong, L Huang, S Li, J Fu, L Zhang, Y Zhang, Q Deng, X Yu
Cell Death Dis, 2020-04-14;11(4):232.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Mechanism of inhibition of the tumor suppressor Patched by Sonic Hedgehog
Proc Natl Acad Sci USA, 2016-09-19;0(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
The hedgehog system machinery controls transforming growth factor-beta-dependent myofibroblastic differentiation in humans: involvement in idiopathic pulmonary fibrosis.
Authors: Cigna N, Farrokhi Moshai E, Brayer S, Marchal-Somme J, Wemeau-Stervinou L, Fabre A, Mal H, Leseche G, Dehoux M, Soler P, Crestani B, Mailleux A
Am J Pathol, 2012-09-29;181(6):2126-37.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P -
A novel synthetic smoothened antagonist transiently inhibits pancreatic adenocarcinoma xenografts in a mouse model.
Authors: Strand MF, Wilson SR, Dembinski JL, Holsworth DD, Khvat A, Okun I, Petersen D, Krauss S
PLoS ONE, 2011-06-15;6(6):e19904.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC
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Used antibody at 20ug/ml overnight at 4C in TBS+1% BSA. In image green=Gli1; blue=DAPI.
