Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all HAND2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HAND2
Met1-Gln217
Accession # P61296

Specificity

Detects human and mouse HAND2 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human HAND1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse HAND2 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human and Mouse HAND2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HAND2

HAND2 (also known as dHAND, Thing-2 and Hed) is a 24 kDa, class B member of the basic helix-loop-helix transcription factors superfamily. HAND2 is essential for cardiac morphogenesis and required for vascular development and regulation of angiogenesis, possibly through VEGF signaling. HAND2 is also implicated in limb development acting as an upstream regulator of sonic hedgehog. Alternative initiation and internal deletions result in at least three potential HAND2 isoforms. Human and mouse HAND2 proteins are 100% identical.

Long Name

Heart And Neural Crest Derivatives Expressed 2

Alternate Names

dHand, Ehand2, Hed, Th2, Thing2

Entrez Gene IDs

9464 (Human); 15111 (Mouse); 64637 (Rat)

Gene Symbol

HAND2

UniProt

P61296

Additional HAND2 Products

Product Documents for Human/Mouse HAND2 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse HAND2 Biotinylated Antibody

For research use only

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