Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunoprecipitation, Co-Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HAND2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HAND2
Met1-Gln217
Accession # P61296

Specificity

Detects human and mouse HAND2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human HAND1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse HAND2 Antibody

Detection of HAND2 in Mouse Embryo.

HAND2 was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Goat Anti-Human/Mouse HAND2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3876) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in developing tongue. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse HAND2 Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo

Western Blot

0.1 µg/mL
Sample: Recombinant Human and Mouse HAND2

Reviewed Applications

Read 1 review rated 5 using AF3876 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HAND2

HAND2 (also known as dHAND, Thing-2 and Hed) is a 24 kDa, class B member of the basic helix-loop-helix transcription factors superfamily. HAND2 is essential for cardiac morphogenesis and required for vascular development and regulation of angiogenesis, possibly through VEGF signaling. HAND2 is also implicated in limb development acting as an upstream regulator of sonic hedgehog. Alternative initiation and internal deletions result in at least three potential HAND2 isoforms. Human and mouse HAND2 proteins are 100% identical.

Long Name

Heart And Neural Crest Derivatives Expressed 2

Alternate Names

dHand, Ehand2, Hed, Th2, Thing2

Entrez Gene IDs

9464 (Human); 15111 (Mouse); 64637 (Rat)

Gene Symbol

HAND2

UniProt

P61296

Additional HAND2 Products

Product Documents for Human/Mouse HAND2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse HAND2 Antibody

For research use only

Citations for Human/Mouse HAND2 Antibody

Customer Reviews for Human/Mouse HAND2 Antibody (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
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  • Human/Mouse HAND2 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E11.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 12/02/2020
    Antibody was used on E12.5 mouse sections (heart). Specific on Hand2+ Right Ventricle. Concentration used - 20ug/mL.
    Human/Mouse HAND2 Antibody AF3876

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Protocols

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