Human/Mouse HAND2 Antibody

Catalog # Availability Size / Price Qty
Detection of HAND2 in Mouse Embryo.
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Product Details
Citations (2)
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Human/Mouse HAND2 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse HAND2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant human HAND1 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human HAND2
Accession # P61296
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human and Mouse HAND2
3-15 µg/mL
Immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of HAND2 in Mouse Embryo. HAND2 was detected in immersion fixed paraffin-embedded 13 d.p.c. sections of Mouse Embryo using Goat Anti-Human/Mouse HAND2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3876) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei in developing tongue. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAND2

HAND2 (also known as dHAND, Thing-2 and Hed) is a 24 kDa, class B member of the basic helix-loop-helix transcription factors superfamily. HAND2 is essential for cardiac morphogenesis and required for vascular development and regulation of angiogenesis, possibly through VEGF signaling. HAND2 is also implicated in limb development acting as an upstream regulator of sonic hedgehog. Alternative initiation and internal deletions result in at least three potential HAND2 isoforms. Human and mouse HAND2 proteins are 100% identical.

Long Name
Heart And Neural Crest Derivatives Expressed 2
Entrez Gene IDs
9464 (Human); 15111 (Mouse); 64637 (Rat)
Alternate Names
BHLHA26; bHLHa26MGC125303; dHand; DHAND2; Ehand2; FLJ16260; HAND2; heart and neural crest derivatives expressed 2; heart, autonomic nervous system and neural crestderivatives-expressed protein 2; Hed; MGC125304; Th2; Thing2

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Citations for Human/Mouse HAND2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Cardiac Reprogramming Factors Synergistically Activate Genome-wide Cardiogenic Stage-Specific Enhancers
    Authors: H Hashimoto, Z Wang, GA Garry, VS Malladi, GA Botten, W Ye, H Zhou, M Osterwalde, DE Dickel, A Visel, N Liu, R Bassel-Dub, EN Olson
    Cell Stem Cell, 2019;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Activin-like kinase 2 functions in peri-implantation uterine signaling in mice and humans.
    Authors: Clementi C, Tripurani S, Large M, Edson M, Creighton C, Hawkins S, Kovanci E, Kaartinen V, Lydon J, Pangas S, DeMayo F, Matzuk M
    PLoS Genet, 2013;9(11):e1003863.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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