Human/Mouse IRF9 Antibody

R&D Systems | Catalog # AF5629

R&D Systems
Discontinued Product
AF5629 has been discontinued. View all IRF9 products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all IRF9 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human IRF9
Ser238-Val393
Accession # Q00978

Specificity

Detects endogenous human and mouse IRF9 in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse IRF9 Antibody

Detection of Human IRF9 antibody by Western Blot.

Detection of Human IRF9 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and 3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Human/Mouse IRF9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5629) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for IRF9 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse IRF9 Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IRF9

IRF9 (interferon regulatory factor 9; also ISGF3-gamma ) is a 48 kDa member of the IRF family of proteins. It is widely expressed, and serves as a component of the ISGF3 complex. Following activation of IFNAR by IFN-alpha / beta, IRF9 is acetylated by CBP on Lys81. IRF9 then associates with activated STAT1 and STAT2 to form an ISGF3 complex that is translocated into the nucleus. Human IRF9 is 393 amino acids (aa) in length. It contains an N-terminal DNA-binding region (aa 11‑112) that contains an NLS (aa 66‑85), plus a STAT-binding domain (aa 200‑393). There are two potential isoform variants. One shows a deletion of aa 218‑331 and 340‑393, while another shows a five aa substitution for aa 217‑393. Over aa 238‑393, human IRF9 shares 79% aa identity with mouse IRF9.

Long Name

Interferon Regulatory Factor 9

Alternate Names

ISGF3, ISGF3G

Entrez Gene IDs

10379 (Human); 16391 (Mouse); 305896 (Rat)

Gene Symbol

IRF9

UniProt

Q00978

Additional IRF9 Products

Product Documents for Human/Mouse IRF9 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse IRF9 Antibody

For research use only

Citations for Human/Mouse IRF9 Antibody

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