Human/Mouse Lefty Antibody

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Product Details
Citations (2)
Supplemental Products
Reviews (1)

Human/Mouse Lefty Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse Lefty in direct ELISAs and Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse Lefty-1
Leu22-Arg135, His6, Leu136-Pro368
Accession # Q64280
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Human Lefty-A (Catalog # 746-LF)
Recombinant Mouse Lefty‑1 (Catalog # 994-LF)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lefty

Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned it the name “lefty”. Two genes exist in mouse (Lefty-1 and Lefty-2) and two in humans (Lefty-A (ebaf) and Lefty-B). By amino acid sequence, mouse Lefty-1 and -2 are more similar to each other (90%) than to either Lefty-A or -B in humans (81 - 82% identical). Lefty contains the six cysteine residues that are conserved among TGF-beta related proteins and that are necessary to form the cysteine-knot structure. However, lefty is distinct from other family members in that it has two RXXR cleavage sites, a longer carboxy terminal sequence, and it lacks the cysteine residue required for intermolecular disulfide linkage. Thus, mature forms of lefty are larger than mature forms of other TGF-beta -related proteins. Mouse Lefty-1 is differentially processed depending on the cells in which it is synthesized, and both processing sites can be utilized.

Lefty homologues have been identified in other vertebrate organisms including chick, frog, and zebrafish. Although the amino acid sequence identity is not well conserved among vertebrate species, the expression pattern of lefty on the left side is well conserved. Lefty-1 is expressed strongly on the left side of the prospective floor plate and weakly in the left half of the lateral plate mesoderm in E8 mice embryos. In all species examined, lefty proteins function in patterning left-right asymmetry of the developing organ systems such as the heart and lung. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor.

  1. Meno, C. et al. (1996) Nature 381:151.
  2. Meno, C. et al. (1997) Genes Cells 2:513.
  3. Meno, C. et al. (1998) Cell 94:287.
  4. Kosaki, K. et al. (1999) Am. J. Hum. Genet. 64:712.
  5. Schier, A.F. and M.M. Shen (1999) Nature 403:385.
  6. Branford, W.W. et al. (2000) Dev. Biol. 223:291.
Entrez Gene IDs
7044 (Human); 13590 (Mouse)
Alternate Names

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Citations for Human/Mouse Lefty Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Induction of Rosette-to-Lumen stage embryoids using reprogramming paradigms in ESCs
    Authors: J Langkabel, A Horne, L Bonaguro, L Holsten, T Hesse, A Knaus, Y Riedel, M Becker, K Händler, T Elmzzahi, K Bassler, N Reusch, LH Yeghiazari, T Pecht, A Saglam, T Ulas, AC Aschenbren, F Kaiser, C Kubaczka, JL Schultze, H Schorle
    Nature Communications, 2021;12(1):7322.
    Species: Mouse
    Sample Types: Organoid
    Applications: ICC
  2. Nodal expression in the uterus of the mouse is regulated by the embryo and correlates with implantation.
    Authors: Park CB, Dufort D,
    Biol. Reprod., 2011;84(6):1103-10.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr


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Isotype Controls

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Secondary Antibodies

Goat IgG (H+L) Antibody


Reviews for Human/Mouse Lefty Antibody

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Human/Mouse Lefty Antibody
By Anonymous on 10/26/2015
Application: ICC/IF Sample Tested: E4.5 ICR mouse embryo Species: Mouse

E4.5 ICR embryos were stained with goat anti-Lefty antibody. DAPI was used for nuclear counterstain. Lefty positive cells were seen in a subset of the primitive endoderm cells.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: PBS-T
Dilution: 1/100