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Human/Mouse LRRTM2 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF5589N Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF5589N-100UG

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Human/Mouse LRRTM2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse LRRTM2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) LRRTM1, rhLRRTM3, and rhLRRTM4 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LRRTM2
Cys34-Arg422
Accession # O43300
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: LRRTM2

LRRTM2 (leucine rich repeat transmembrane protein # 2) is a 59 kDa (predicted) member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM2 is found in deep neurons of the hippocampus (CA1-CA3), dentate gyrus granule cells, and cerebellar granular cells, and in almost all peripheral tissues at some level. Mature human LRRTM2 is 483 amino acids (aa) in length. The extracellular region contains ten LRRs (aa 61‑299), followed by a 73 aa cytoplasmic tail (aa 444‑516). Over aa 34‑422. human LRRTM2 shares 98% aa identity with mouse LRRTM2.

Long Name
Leucine Rich Repeat Transmembrane Neuronal 2
Entrez Gene IDs
26045 (Human); 107065 (Mouse); 685472 (Rat)
Alternate Names
leucine-rich repeat transmembrane neuronal protein 2; leucine rich repeat transmembrane neuronal 2; LRRN2; LRRTM2

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