LRRTM2 in Mouse Embryo. LRRTM2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of Human/Mouse LRRTM2 by Western Blot. Western blot shows lysates of human brain cortex and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRTM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
LRRTM2 in Mouse Neural Tube. LRRTM2 was detected in immersion fixed frozen sections of mouse neural tube (E9.5) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
LRRTM2 (leucine rich repeat transmembrane protein # 2) is a 59 kDa (predicted) member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM2 is found in deep neurons of the hippocampus (CA1-CA3), dentate gyrus granule cells, and cerebellar granular cells, and in almost all peripheral tissues at some level. Mature human LRRTM2 is 483 amino acids (aa) in length. The extracellular region contains ten LRRs (aa 61‑299), followed by a 73 aa cytoplasmic tail (aa 444‑516). Over aa 34‑422. human LRRTM2 shares 98% aa identity with mouse LRRTM2.
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