Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Neutralization, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all LRRTM2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human LRRTM2
Cys34-Arg422
Accession # O43300

Specificity

Detects human and mouse LRRTM2 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) LRRTM1, rhLRRTM3, and rhLRRTM4 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse LRRTM2 Antibody

LRRTM2 antibody in Embryonic Mouse Neural Tube by Immunohistochemistry (IHC-Fr).

LRRTM2 in Embryonic Mouse Neural Tube.

LRRTM2 was detected in immersion fixed frozen sections of embryonic mouse neural tube (E10.5) using 10 µg/mL Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of Human/Mouse LRRTM2 antibody by Western Blot.

Detection of Human/Mouse LRRTM2 by Western Blot.

Western blot shows lysates of human brain cortex and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LRRTM2 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

LRRTM2 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

LRRTM2 in Mouse Embryo.

LRRTM2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

LRRTM2 antibody in Mouse Neural Tube by Immunohistochemistry (IHC-Fr).

LRRTM2 in Mouse Neural Tube.

LRRTM2 was detected in immersion fixed frozen sections of mouse neural tube (E9.5) using Sheep Anti-Human/Mouse LRRTM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5589) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse LRRTM2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of embryonic mouse neural tube (E10.5)

Western Blot

1 µg/mL
Sample: Human brain cortex and mouse brain tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: LRRTM2

LRRTM2 (leucine rich repeat transmembrane protein # 2) is a 59 kDa (predicted) member of the LRRTM family of proteins. All members of this family are type I transmembrane proteins that contain 10 external LRR repeats plus a short cytoplasmic tail that binds PDF motifs. LRRTM2 is found in deep neurons of the hippocampus (CA1-CA3), dentate gyrus granule cells, and cerebellar granular cells, and in almost all peripheral tissues at some level. Mature human LRRTM2 is 483 amino acids (aa) in length. The extracellular region contains ten LRRs (aa 61‑299), followed by a 73 aa cytoplasmic tail (aa 444‑516). Over aa 34‑422. human LRRTM2 shares 98% aa identity with mouse LRRTM2.

Long Name

Leucine Rich Repeat Transmembrane Neuronal 2

Alternate Names

LRRN2

Entrez Gene IDs

26045 (Human); 107065 (Mouse); 685472 (Rat)

Gene Symbol

LRRTM2

UniProt

O43300

Additional LRRTM2 Products

Product Documents for Human/Mouse LRRTM2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse LRRTM2 Antibody

For research use only

Related Research Areas

Activin Family

Citations for Human/Mouse LRRTM2 Antibody

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Protocols

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