Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Label

Alexa Fluor 405 (Excitation = 405 nm, Emission = 421 nm)

Antibody Source

Polyclonal Goat IgG
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Product Specifications

Immunogen

E. coli-derived recombinant human MCM2
Asp143-Ser296
Accession # P49736

Specificity

Detects human and mouse MCM2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human/Mouse MCM2 Alexa Fluor® 405‑conjugated Antibody

Application
Recommended Usage

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MCM2

CM2 (Minichromosome maintenance protein 2; also BM28) is a member of the MCM family of proteins. Although its predicted MW is 102 kDa, it runs anomalously at 125 kDa in SDS‑PAGE. MCM2 forms a large, 450‑600 kDa prereplication complex with MCM3 through 7 plus HBO1, and associates with the ORC to initiate DNA synthesis. Human MCM2 is 904 amino acids (aa) in length (Accession # P49736). It contains one C4‑type zinc finger region (aa 329‑355) and a P‑loop NTPase region (aa 485‑659) and at least 12 Ser/Thr phosphorylation sites in the N‑terminal one‑third of the molecule. MCM2 has several potential isoforms. There is an alternate start site at Met10 with or without a 47 aa substitution for aa 109‑157, a deletion of aa 31‑126, a Gly substitution for aa 113‑132 coupled with a 69 aa insertion after Lys476, and a seven aa substitution for aa 1‑137. Over aa 143‑296, human MCM2 shares 99% aa identity with mouse MCM2.

Long Name

Minichromosome Maintenance Protein 2

Alternate Names

BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin

Entrez Gene IDs

4171 (Human); 17216 (Mouse); 312538 (Rat)

Gene Symbol

MCM2

UniProt

Additional MCM2 Products

Product Documents for Human/Mouse MCM2 Alexa Fluor® 405‑conjugated Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse MCM2 Alexa Fluor® 405‑conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

Related Research Areas

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Protocols

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