Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MCM2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MCM2
Asp143-Ser296
Accession # P49736

Specificity

Detects human and mouse MCM2 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse MCM2 Antibody

Detection of Human/Mouse MCM2 antibody by Western Blot.

Detection of Human/Mouse MCM2 by Western Blot.

Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse MCM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for MCM2 at approximately 125kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MCM2 antibody in Human Colon by Immunohistochemistry (IHC-P).

MCM2 in Human Colon.

MCM2 was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human/Mouse MCM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5778) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human/Mouse MCM2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

1 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and DA3 mouse myeloma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MCM2

CM2 (Minichromosome maintenance protein 2; also BM28) is a member of the MCM family of proteins. Although its predicted MW is 102 kDa, it runs anomalously at 125 kDa in SDS‑PAGE. MCM2 forms a large, 450‑600 kDa prereplication complex with MCM3 through 7 plus HBO1, and associates with the ORC to initiate DNA synthesis. Human MCM2 is 904 amino acids (aa) in length (Accession # P49736). It contains one C4‑type zinc finger region (aa 329‑355) and a P‑loop NTPase region (aa 485‑659) and at least 12 Ser/Thr phosphorylation sites in the N‑terminal one‑third of the molecule. MCM2 has several potential isoforms. There is an alternate start site at Met10 with or without a 47 aa substitution for aa 109‑157, a deletion of aa 31‑126, a Gly substitution for aa 113‑132 coupled with a 69 aa insertion after Lys476, and a seven aa substitution for aa 1‑137. Over aa 143‑296, human MCM2 shares 99% aa identity with mouse MCM2.

Long Name

Minichromosome Maintenance Protein 2

Alternate Names

BM28, CCNL1, cdc19, CDCL1, D3S3194, Mitotin

Entrez Gene IDs

4171 (Human); 17216 (Mouse); 312538 (Rat)

Gene Symbol

MCM2

UniProt

P49736

Additional MCM2 Products

Product Documents for Human/Mouse MCM2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse MCM2 Antibody

For research use only

Related Research Areas

Cell Cycle

Citations for Human/Mouse MCM2 Antibody

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Protocols

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