Human/Mouse MCM2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human/Mouse MCM2 by Western Blot.
2 Images
Product Details
Citations (2)
Supplemental Products

Human/Mouse MCM2 Antibody Summary

Species Reactivity
Human, Mouse
Detects human and mouse MCM2 in Western blots.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human MCM2
Accession # P49736
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human/Mouse MCM2 antibody by Western Blot. View Larger

Detection of Human/Mouse MCM2 by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and DA3 mouse myeloma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse MCM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for MCM2 at approximately 125kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry MCM2 antibody in Human Colon by Immunohistochemistry (IHC-P). View Larger

MCM2 in Human Colon. MCM2 was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human/Mouse MCM2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5778) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MCM2

CM2 (Minichromosome maintenance protein 2; also BM28) is a member of the MCM family of proteins. Although its predicted MW is 102 kDa, it runs anomalously at 125 kDa in SDS‑PAGE. MCM2 forms a large, 450‑600 kDa prereplication complex with MCM3 through 7 plus HBO1, and associates with the ORC to initiate DNA synthesis. Human MCM2 is 904 amino acids (aa) in length (Accession # P49736). It contains one C4‑type zinc finger region (aa 329‑355) and a P‑loop NTPase region (aa 485‑659) and at least 12 Ser/Thr phosphorylation sites in the N‑terminal one‑third of the molecule. MCM2 has several potential isoforms. There is an alternate start site at Met10 with or without a 47 aa substitution for aa 109‑157, a deletion of aa 31‑126, a Gly substitution for aa 113‑132 coupled with a 69 aa insertion after Lys476, and a seven aa substitution for aa 1‑137. Over aa 143‑296, human MCM2 shares 99% aa identity with mouse MCM2.

Long Name
Minichromosome Maintenance Protein 2
Entrez Gene IDs
4171 (Human); 17216 (Mouse); 312538 (Rat)
Alternate Names
BM28; BM28MGC10606; CCNL 1; CCNL1; cdc19; CDCL1; CDCL1mitotin; D3S3194; EC; KIAA0030MITOTIN; MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae); MCM2; minichromosome maintenance complex component 2; minichromosome maintenance deficient (S. cerevisiae) 2 (mitotin); minichromosome maintenance deficient 2 (mitotin); Mitotin

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Citations for Human/Mouse MCM2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Overexpression of microRNA-367 inhibits angiogenesis in ovarian cancer by downregulating the expression of LPA1
    Authors: Q Zheng, X Dai, W Fang, Y Zheng, J Zhang, Y Liu, D Gu
    Cancer Cell Int, 2020;20(0):476.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. An Adeno-Associated Virus-Based Toolkit for Preferential Targeting and Manipulating Quiescent Neural Stem Cells in the Adult Hippocampus
    Authors: AJ Crowther, SA Lim, B Asrican, BH Albright, J Wooten, CY Yeh, H Bao, DH Cerri, J Hu, YY Ian Shih, A Asokan, J Song
    Stem Cell Reports, 2018;10(3):1146-1159.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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