Detection of Myogenin in C2C12 Mouse Cell Line by Flow Cytometry. C2C12 mouse myoblast cell line was stained with Mouse Anti-Human/Mouse Myogenin Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # IC6686G, filled histogram) or isotype control antibody (Catalog # IC002G, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Myogenin, also known as MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the Myogenic Regulatory Factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa sequence identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.
Entrez Gene IDs:
4656 (Human); 17928 (Mouse); 29148 (Rat)
BHLHC3; bHLHc3Myogenic factor-4; myogenin; Class C basic helix-loop-helix protein 3; MYF4; myf-4; MYF4myogenin; MYOG; Myogenic factor 4; myogenin (myogenic factor 4); Myogenin
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