Human/Mouse Myogenin Antibody Summary
Accession # P15173
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Myogenin in C2C12 Mouse Myoblast Cells. Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cells using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB6686) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to select differentiating nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Myogenin, also called MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the myogenic regulatory factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.
Citation for Human/Mouse Myogenin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Skeletal Muscle Resident Progenitor Cells Coexpress Mesenchymal and Myogenic Markers and Are Not Affected by Chronic Heart Failure-Induced Dysregulations
Authors: RI Dmitrieva, TA Lelyavina, MY Komarova, VL Galenko, OA Ivanova, PA Tikanova, NV Khromova, AS Golovkin, MA Bortsova, A Sergushich, MY Sitnikova, AA Kostareva
Stem Cells Int, 2019;2019(0):5690345.
Sample Types: Whole Tissue
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