Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 671038
View all Myogenin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Myogenin
Ser124-Asn224
Accession # P15173

Specificity

Detects human Myogenin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse Myogenin Antibody

Myogenin antibody in C2C12 Mouse Myoblast Cells by Immunocytochemistry (ICC).

Myogenin in C2C12 Mouse Myoblast Cells.

Myogenin was detected in immersion fixed differentiated C2C12 mouse myoblast cells using Mouse Anti-Human Myogenin Monoclonal Antibody (Catalog # MAB6686) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to select differentiating nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Myogenin in C2C12 cells by Flow Cytometry.

C2C12 cells were stained with Mouse Anti-Human/Mouse Myogenin Monoclonal Antibody (Catalog # MAB6686, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse Myogenin Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: C2C12 mouse myoblast cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion-fixed differentiated C2C12 mouse myoblast cells

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Myogenin

Myogenin, also called MYF4, is a 34 kDa, 224 amino acid (aa) basic-HLH transcription factor member of the myogenic regulatory factor (MRF) family. It induces fibroblastic differentiation to myoblasts and is expressed most highly early in the skeletal muscle differentiation process. Within the region used as an immunogen, human myogenin shares 93% and 91% aa identity with mouse and rat myogenin, respectively. Isoforms may exist that diverge at aa 155 (246 aa form) or that are truncated at aa 215.

Alternate Names

BHLHC3, MYF4, MYOG

Entrez Gene IDs

4656 (Human); 17928 (Mouse); 29148 (Rat)

Gene Symbol

MYOG

UniProt

P15173

Additional Myogenin Products

Product Documents for Human/Mouse Myogenin Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse Myogenin Antibody

For research use only

Citations for Human/Mouse Myogenin Antibody

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Protocols

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