Human/Mouse NRAGE Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB38352U Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB38352U-100UG

Save 15% on Select RUO Reagents. See Details.

All NRAGE Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse NRAGE Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human NRAGE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human MAGE-A4 is observed.

Source

Monoclonal Mouse IgG2b Clone # 613441

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human NRAGE
Arg73-Asn254
Accession # Q9Y5V3

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: NRAGE

NRAGE, also known as MAGE-D1 or Dlxin-1, is a ubiquitously expressed cytosolic protein that is a member of the melanoma-associated antigen (MAGE) family. The 778 aa human NRAGE protein contains a segment with 22 repeats of a W(P/Q)xPxx motif, followed by a MAGE domain. Human and mouse NRAGE share 87% aa identity. In the brain, NRAGE interacts with p75NTR, blocks cell cycle progression, and facilitates NGF-dependent apoptosis.

Long Name

Neurotrophin Receptor-interacting MAGE Homolog

Entrez Gene IDs

9500 (Human); 94275 (Mouse); 84469 (Rat)

Alternate Names

Dlxin-1; MAGE tumor antigen CCF; MAGED1; melanoma antigen family D, 1; melanoma-associated antigen D1; Neurotrophin receptor-interacting MAGE homolog; NRAGE MAGE-D1 antigen; NRAGE