Detection of Human, Mouse, and Rat NRAGE by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB38352) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NRAGE at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
NRAGE in C2C12 Mouse Cell Line.
NRAGE was detected in immersion fixed C2C12 mouse myoblast cell line using Mouse Anti-Human NRAGE Monoclonal Antibody (Catalog # MAB38352) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NRAGE, also known as MAGE-D1 or Dlxin-1, is a ubiquitously expressed cytosolic protein that is a member of the melanoma-associated antigen (MAGE) family. The 778 aa human NRAGE protein contains a segment with 22 repeats of a W(P/Q)xPxx motif, followed by a MAGE domain. Human and mouse NRAGE share 87% aa identity. In the brain, NRAGE interacts with p75NTR, blocks cell cycle progression, and facilitates NGF-dependent apoptosis.
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