Human/Mouse PDX-1/IPF1 APC-conjugated Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse PDX-1 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 267712
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PDX-1
    Ala91-Arg283
    Accession # P52945
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Allophycocyanin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX‑1/IPF1 APC‑conjugated Monoclonal Antibody (Catalog # IC2419A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PDX-1/IPF1
PDX-1 (Pancreatic-duodenal Homeobox Factor-1), also known as IDX-1 and STF-1 is a 31 kDa member of the IPF-1 (Insulin Promoter Factor) subfamily, Antp (antennapedia) family of molecules. Human PDX-1 is 283 amino acids (aa) in length, and although its predicted MW is approximately 30 kDa, it runs anomalously in SDS-PAGE at 40-46 kDa. PDX-1 possesses one transctivation domain (aa 13-73) and one DNA-binding homeodomain (aa 146-205) that contains an NLS between aa 197-203. Phosphorylation is known to occur on Ser61 and Ser269, and methylation is found on Lys131. PDX-1 drives the development of the embryonic pancreas, and post-natally it is found in both beta - and δ (somatostatin) islet cells. This transcription factor complexes with multiple cofactors (Set9; NeuroD1; MafA; HMGA1) in its regulation of beta -cell division and insulin synthesis, and serves principally as an RNA pol II binding modulator. Over aa 91-283, human and mouse PDX-1 share 87% aa sequence identity.
  • Long Name:
    Pancreas/Duodenum Homeobox-1
  • Entrez Gene IDs:
    3651 (Human); 18609 (Mouse)
  • Alternate Names:
    Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B APC-conjugated Antibody

Ctrl IC0041A 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Human/Mouse PDX-1/IPF1 APC-conjugated Antibody?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee

Monoclonal
Antibody
Panels For
Screening

Learn More
Monoclonal Antibodies

Feedback

Customer Information