Detection of PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line by Flow Cytometry.
beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX‑1/IPF1 APC‑conjugated Monoclonal Antibody (Catalog # IC2419A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
PDX-1 (Pancreatic-duodenal Homeobox Factor-1), also known as IDX-1 and STF-1 is a 31 kDa member of the IPF-1 (Insulin Promoter Factor) subfamily, Antp (antennapedia) family of molecules. Human PDX-1 is 283 amino acids (aa) in length, and although its predicted MW is approximately 30 kDa, it runs anomalously in SDS-PAGE at 40-46 kDa. PDX-1 possesses one transctivation domain (aa 13-73) and one DNA-binding homeodomain (aa 146-205) that contains an NLS between aa 197-203. Phosphorylation is known to occur on Ser61 and Ser269, and methylation is found on Lys131. PDX-1 drives the development of the embryonic pancreas, and post-natally it is found in both beta - and δ (somatostatin) islet cells. This transcription factor complexes with multiple cofactors (Set9; NeuroD1; MafA; HMGA1) in its regulation of beta -cell division and insulin synthesis, and serves principally as an RNA pol II binding modulator. Over aa 91-283, human and mouse PDX-1 share 87% aa sequence identity.
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