Human/Mouse PDX-1/IPF1 Antibody
Human/Mouse PDX-1/IPF1 Antibody Summary
Ala91-Arg283
Accession # P52945
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse PDX‑1/IPF1 by Western Blot. Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDX-1/IPF1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line. PDX-1/IPF1 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. PDX-1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
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Detection of PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX-1/IPF1 Monoclonal Antibody (Catalog # MAB2419, filled histogram) or isotype control antibody (MAB0041, open histogram) followed by anti-Mouse IgG PE-conjugated secondary antibody (F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). View our protocol for Staining Intracellular Molecules.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDX-1/IPF1
Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function.
Product Datasheets
Citations for Human/Mouse PDX-1/IPF1 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Intrinsically disordered substrates dictate SPOP subnuclear localization and ubiquitination activity
Authors: ET Usher, N Sabri, R Rohac, AK Boal, T Mittag, SA Showalter
The Journal of Biological Chemistry, 2021;0(0):100693.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot -
A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells
Authors: P Saxena, BC Heng, P Bai, M Folcher, H Zulewski, M Fussenegge
Nat Commun, 2016;7(0):11247.
Species: Human
Sample Types: Whole Cells
Applications: IHC -
Redifferentiation of adult human beta cells expanded in vitro by inhibition of the WNT pathway.
Authors: Lenz A, Toren-Haritan G, Efrat S
PLoS ONE, 2014;9(11):e112914.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Peroxisome proliferator-activated receptor gamma activation restores islet function in diabetic mice through reduction of endoplasmic reticulum stress and maintenance of euchromatin structure.
Authors: Evans-Molina C, Robbins RD, Kono T, Tersey SA, Vestermark GL, Nunemaker CS, Garmey JC, Deering TG, Keller SR, Maier B, Mirmira RG
Mol. Cell. Biol., 2009;29(8):2053-67.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Generation of insulin-producing cells from PDX-1 gene-modified human mesenchymal stem cells.
Authors: Li Y, Zhang R, Qiao H, Zhang H, Wang Y, Yuan H, Liu Q, Liu D, Chen L, Pei X
J. Cell. Physiol., 2007;211(1):36-44.
Species: Human
Sample Types: Whole Cells
Applications: ICC -
Betacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cells.
Authors: Cho YM, Lim JM, Yoo DH, Kim JH, Chung SS, Park SG, Kim TH, Oh SK, Choi YM, Moon SY, Park KS, Lee HK
Biochem. Biophys. Res. Commun., 2007;366(1):129-34.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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