Human/Mouse PDX-1/IPF1 Antibody

Catalog # Availability Size / Price Qty
MAB2419
MAB2419-SP
Detection of Mouse PDX‑1/IPF1 by Western Blot.
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Product Details
Citations (5)
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Human/Mouse PDX-1/IPF1 Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse PDX-1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 267712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PDX-1
Ala91-Arg283
Accession # P52945
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Mouse PDX‑1/IPF1 by Western Blot. View Larger

Detection of Mouse PDX‑1/IPF1 by Western Blot. Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDX‑1/IPF1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry PDX‑1/IPF1 in  beta TC‑6 Mouse Cell Line. View Larger

PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line. PDX‑1/IPF1 was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. View Larger

PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Mouse Anti-Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDX-1/IPF1

Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function.

Long Name
Pancreas/Duodenum Homeobox-1
Entrez Gene IDs
3651 (Human); 18609 (Mouse)
Alternate Names
Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1

Product Datasheets

Citations for Human/Mouse PDX-1/IPF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells
    Authors: P Saxena, BC Heng, P Bai, M Folcher, H Zulewski, M Fussenegge
    Nat Commun, 2016;7(0):11247.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC - PFA fixed
  2. Redifferentiation of adult human beta cells expanded in vitro by inhibition of the WNT pathway.
    Authors: Lenz A, Toren-Haritan G, Efrat S
    PLoS ONE, 2014;9(11):e112914.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  3. Peroxisome proliferator-activated receptor gamma activation restores islet function in diabetic mice through reduction of endoplasmic reticulum stress and maintenance of euchromatin structure.
    Authors: Evans-Molina C, Robbins RD, Kono T, Tersey SA, Vestermark GL, Nunemaker CS, Garmey JC, Deering TG, Keller SR, Maier B, Mirmira RG
    Mol. Cell. Biol., 2009;29(8):2053-67.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  4. Generation of insulin-producing cells from PDX-1 gene-modified human mesenchymal stem cells.
    Authors: Li Y, Zhang R, Qiao H, Zhang H, Wang Y, Yuan H, Liu Q, Liu D, Chen L, Pei X
    J. Cell. Physiol., 2007;211(1):36-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  5. Betacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cells.
    Authors: Cho YM, Lim JM, Yoo DH, Kim JH, Chung SS, Park SG, Kim TH, Oh SK, Choi YM, Moon SY, Park KS, Lee HK
    Biochem. Biophys. Res. Commun., 2007;366(1):129-34.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2B Antibody

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