Human/Mouse PDX-1/IPF1 Antibody

(5 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse PDX-1 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 267712
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PDX-1
    Ala91-Arg283
    Accession # P52945
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Immersion fixed paraffin-embedded sections of human pancreatic cancer tissue
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Mouse PDX‑1/IPF1 by Western Blot. Western blot shows lysates of beta TC‑6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDX‑1/IPF1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line. PDX‑1/IPF1 was detected in immersion fixed beta TC‑6 mouse beta cell insulinoma cell line using Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry

PDX‑1/IPF1 in BG01V Human Embryonic Stem Cells. PDX‑1/IPF1 was detected in immersion fixed BG01V human embryonic stem cells differentiated into pancreatic progenitor cells using Mouse Anti-Human/Mouse PDX‑1/IPF1 Monoclonal Antibody (Catalog # MAB2419) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDX-1/IPF1
Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function.
  • Long Name:
    Pancreas/Duodenum Homeobox-1
  • Entrez Gene IDs:
    3651 (Human); 18609 (Mouse)
  • Alternate Names:
    Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. A programmable synthetic lineage-control network that differentiates human IPSCs into glucose-sensitive insulin-secreting beta-like cells
    Authors: P Saxena, BC Heng, P Bai, M Folcher, H Zulewski, M Fussenegge
    Nat Commun, 2016;7(0):11247.
    Species: Human
    Sample Type: Whole Cells
    Application: IHC - PFA fixed
  2. Redifferentiation of adult human beta cells expanded in vitro by inhibition of the WNT pathway.
    Authors: Lenz A, Toren-Haritan G, Efrat S
    PLoS ONE, 2014;9(11):e112914.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  3. Peroxisome proliferator-activated receptor gamma activation restores islet function in diabetic mice through reduction of endoplasmic reticulum stress and maintenance of euchromatin structure.
    Authors: Evans-Molina C, Robbins RD, Kono T, Tersey SA, Vestermark GL, Nunemaker CS, Garmey JC, Deering TG, Keller SR, Maier B, Mirmira RG
    Mol. Cell. Biol., 2009;29(8):2053-67.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  4. Generation of insulin-producing cells from PDX-1 gene-modified human mesenchymal stem cells.
    Authors: Li Y, Zhang R, Qiao H, Zhang H, Wang Y, Yuan H, Liu Q, Liu D, Chen L, Pei X
    J. Cell. Physiol., 2007;211(1):36-44.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  5. Betacellulin and nicotinamide sustain PDX1 expression and induce pancreatic beta-cell differentiation in human embryonic stem cells.
    Authors: Cho YM, Lim JM, Yoo DH, Kim JH, Chung SS, Park SG, Kim TH, Oh SK, Choi YM, Moon SY, Park KS, Lee HK
    Biochem. Biophys. Res. Commun., 2007;366(1):129-34.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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