Human/Mouse PDX-1/IPF1 PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse PDX-1/IPF1 in Western blots.
  • Source
    Monoclonal Mouse IgG2B Clone # 267712
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PDX-1/IPF1
    Ala91-Arg283
    Accession # P52945
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Intracellular Staining by Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Intracellular Staining by Flow Cytometry
Detection of PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC‑6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX‑1/IPF1 PE‑conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Intracellular Staining by Flow Cytometry
Detection of PDX‑1/IPF1 in BG01V Human Stem Cells Differentiated into Pancreatic Progenitor Cells by Flow Cytometry. BG01V human embryonic stem cells either (A) untreated or (B) differentiated into pancreatic progenitor cells were stained with Mouse Anti-Human/Mouse PDX‑1/IPF1 PE‑conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PDX-1/IPF1
Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function.
  • Long Name:
    Pancreas/Duodenum Homeobox-1
  • Entrez Gene IDs:
    3651 (Human); 18609 (Mouse)
  • Alternate Names:
    Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. In vitro quantitative and relative gene expression analysis of pancreatic transcription factors Pdx-1, Ngn-3, Isl-1, Pax-4, Pax-6 and Nkx-6.1 in trans-differentiated human hepatic progenitors.
    Authors: Vishwakarma S, Rahamathulla S, Bardia A, Tiwari S, Srinivas G, Raj A, Tripura C, Sandhya A, Habeeb M, Khan A, Pande G, Reddy K, Reddy P
    J Diabetes Investig, 2014;5(5):492-500.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.
    Authors: Kornete, Mara, Beauchemin, Hugues, Polychronakos, Constant, Piccirillo, Ciriaco
    Autoimmunity, 2013;46(4):259-68.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2B PE-conjugated Antibody

Ctrl IC0041P 20  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Permeabilization/Wash Buffer I (1X)

Flow FC005 5
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Flow Cytometry Fixation Buffer (1X)

Flow FC004 3
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Flow Cytometry Fixation & Permeabilization Buffer Kit I

Flow FC009 3
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Flow Cytometry Fixation/Permeabilization Buffer I (1X)

Flow FC007 2
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