Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Intracellular Staining by Flow Cytometry

Cited:

Immunohistochemistry-Frozen, Flow Cytometry

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 267712
View all PDX-1/IPF1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PDX-1/IPF1
Ala91-Arg283
Accession # P52945

Specificity

Detects human and mouse PDX-1/IPF1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse PDX‑1/IPF1 PE‑conjugated Antibody

Detection of PDX-1/IPF1 antibody in beta TC-6 Mouse Cell Line antibody by Flow Cytometry.

Detection of PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line by Flow Cytometry.

beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX-1/IPF1 PE-conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Detection of PDX-1/IPF1 antibody in BG01V Human Stem Cells Differentiated into Pancreatic Progenitor Cells antibody by Flow Cytometry.

Detection of PDX‑1/IPF1 in BG01V Human Stem Cells Differentiated into Pancreatic Progenitor Cells by Flow Cytometry.

BG01V human embryonic stem cells either (A) untreated or (B) differentiated into pancreatic progenitor cells were stained with Mouse Anti-Human/Mouse PDX-1/IPF1 PE-conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Applications for Human/Mouse PDX‑1/IPF1 PE‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

10 µL/106 cells
Sample: beta TC‑6 mouse beta cell insulinoma cell line or BG01V human embryonic stem cells differentiated into pancreatic progenitor cells  were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005)

Spectra Viewer

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PDX-1/IPF1

Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function.

Long Name

Pancreas/Duodenum Homeobox-1

Alternate Names

IDX1, IPF1

Entrez Gene IDs

3651 (Human); 18609 (Mouse)

Gene Symbol

PDX1

UniProt

P52945

Additional PDX-1/IPF1 Products

Product Documents for Human/Mouse PDX‑1/IPF1 PE‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse PDX‑1/IPF1 PE‑conjugated Antibody

For research use only

Citations for Human/Mouse PDX‑1/IPF1 PE‑conjugated Antibody

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