Human/Mouse PDX-1/IPF1 PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2419P
Human/Mouse PDX-1/IPF1 PE-conjugated Antibody in Data
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Product Details
Citations (2)
FAQs
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Human/Mouse PDX-1/IPF1 PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse PDX-1/IPF1 in Western blots.
Source
Monoclonal Mouse IgG2B Clone # 267712
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PDX-1/IPF1
Ala91-Arg283
Accession # P52945
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Intracellular Staining by Flow Cytometry Detection of PDX-1/IPF1 antibody in  beta TC-6 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line by Flow Cytometry. beta TC-6 mouse beta cell insulinoma cell line was stained with Mouse Anti-Human/Mouse PDX-1/IPF1 PE-conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Intracellular Staining by Flow Cytometry Detection of PDX-1/IPF1 antibody in BG01V Human Stem Cells Differentiated into Pancreatic Progenitor Cells antibody by Flow Cytometry. View Larger

Detection of PDX‑1/IPF1 in BG01V Human Stem Cells Differentiated into Pancreatic Progenitor Cells by Flow Cytometry. BG01V human embryonic stem cells either (A) untreated or (B) differentiated into pancreatic progenitor cells were stained with Mouse Anti-Human/Mouse PDX-1/IPF1 PE-conjugated Monoclonal Antibody (Catalog # IC2419P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PDX-1/IPF1

Pancreatic-Duodenal Homeobox Factor-1 (PDX-1), also known as Insulin Promoter Factor 1 (IPF1), is a homeodomain transcription factor essential for pancreatic development and mature pancreatic beta cell function.

Long Name
Pancreas/Duodenum Homeobox-1
Entrez Gene IDs
3651 (Human); 18609 (Mouse)
Alternate Names
Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1

Product Datasheets

Citations for Human/Mouse PDX-1/IPF1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. In vitro quantitative and relative gene expression analysis of pancreatic transcription factors Pdx-1, Ngn-3, Isl-1, Pax-4, Pax-6 and Nkx-6.1 in trans-differentiated human hepatic progenitors.
    Authors: Vishwakarma S, Rahamathulla S, Bardia A, Tiwari S, Srinivas G, Raj A, Tripura C, Sandhya A, Habeeb M, Khan A, Pande G, Reddy K, Reddy P
    J Diabetes Investig, 2014;5(5):492-500.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Pancreatic islet cell phenotype and endocrine function throughout diabetes development in non-obese diabetic mice.
    Authors: Kornete, Mara, Beauchemin, Hugues, Polychronakos, Constant, Piccirillo, Ciriaco
    Autoimmunity, 2013;46(4):259-68.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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