Human/Mouse Phospho- Histone H2AX (S139) Antibody

Catalog # Availability Size / Price Qty
4418-APC-100
Detection of Phospho-Histone H2AX (S139) in Western blot.
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Product Details
Citations (2)
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Human/Mouse Phospho- Histone H2AX (S139) Antibody Summary

Species Reactivity
Human, Mouse
Specificity
Detects human and mouse Histone H2AX when phosphorylated at S139 in Western blots. This antibody detects only double-strand DNA breaks.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human Histone H2AX S139 site
Formulation
Supplied as a solution containing PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:2500 dilution
Wehi cells were treated with etoposide
Immunocytochemistry
1.7-15 µg/mL
mmersion fixed C2C12 mouse myoblast cell line stimulated with etoposide

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot View Larger

Detection of Phospho-Histone H2AX (S139) in Western blot. Wehi cells were treated with (T)and without (H) 25 μM etoposide for 4 hours.Cells were lysed in Tris-Glycine SDS samplebuffer at the concentration 1 x 107cells/ml and10 μl of each lysate were loaded per well of 4-20% Tris-Glycine gel. Proteins were transferredonto an Immobilon FL membrane and Phospho-H2AX (S139)was detected with anti-phosphorylated HistoneH2AX antibody (Cat# 4418-APC-020, 1:2500) followed by IR800-conjugated anti-rabbit secondary antibody.

Immunocytochemistry View Larger

Phospho-Histone H2AX (S139) in C2C12 Mouse Cell Line. Phospho-Histone H2AX (S139) was detected in immersion fixed C2C12 mouse myoblast cell line stimulated with etoposide (positive, left panel) and C2C12 mouse myoblast cell line (negative, right panel) using Rabbit Anti-Human/Mouse Phospho- Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # 4418-APC-020) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: Histone H2AX

Histone H2AX is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed ( gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.

Entrez Gene IDs
3014 (Human)
Alternate Names
H2A.X; H2A/X; H2AFX; Histone H2AX

Product Datasheets

Citations for Human/Mouse Phospho- Histone H2AX (S139) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Genetic determinants of cellular addiction to DNA polymerase theta
    Authors: W Feng, DA Simpson, J Carvajal-G, BA Price, RJ Kumar, LE Mose, RD Wood, N Rashid, JE Purvis, JS Parker, DA Ramsden, GP Gupta
    Nat Commun, 2019;10(1):4286.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  2. Cytological and proteomic analyses of floral buds reveal an altered atlas of meiosis in autopolyploid Brassica rapa
    Authors: Y Yang, F Wei, J Braynen, X Wei, B Tian, G Shi, G Cao, J Yuan, X Zhang
    Cell Biosci, 2019;9(0):49.
    Species: B. Rapa
    Sample Types: Inflorescences
    Applications: ICC

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