Human/Mouse Phospho- Histone H2AX (S139) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Phospho-Histone H2AX (S139) in Western blot. Wehi cells were treated with (T)and without (H) 25 μM etoposide for 4 hours.Cells were lysed in Tris-Glycine SDS samplebuffer at the concentration 1 x 107cells/ml and10 μl of each lysate were loaded per well of 4-20% Tris-Glycine gel. Proteins were transferredonto an Immobilon FL membrane and Phospho-H2AX (S139)was detected with anti-phosphorylated HistoneH2AX antibody (Cat# 4418-APC-020, 1:2500) followed by IR800-conjugated anti-rabbit secondary antibody.
Phospho-Histone H2AX (S139) in C2C12 Mouse Cell Line. Phospho-Histone H2AX (S139) was detected in immersion fixed C2C12 mouse myoblast cell line stimulated with etoposide (positive, left panel) and C2C12 mouse myoblast cell line (negative, right panel) using Rabbit Anti-Human/Mouse Phospho- Histone H2AX (S139) Antigen Affinity-purified Polyclonal Antibody (Catalog # 4418-APC-020) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Histone H2AX
Histone H2AX is phosphorylated at S139 in cells exposed to DNA double-strand break-inducing agents, such as ionizing radiation. The S139 phosphorylated H2AX, termed ( gamma -H2AX, marks the site of DNA double-strand breaks and serves to recruit cell cycle checkpoint and DNA repair factors to the site of damage.
Citations for Human/Mouse Phospho- Histone H2AX (S139) Antibody
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Genetic determinants of cellular addiction to DNA polymerase theta
Authors: W Feng, DA Simpson, J Carvajal-G, BA Price, RJ Kumar, LE Mose, RD Wood, N Rashid, JE Purvis, JS Parker, DA Ramsden, GP Gupta
Nat Commun, 2019;10(1):4286.
Sample Types: Whole Cells
Cytological and proteomic analyses of floral buds reveal an altered atlas of meiosis in autopolyploid Brassica rapa
Authors: Y Yang, F Wei, J Braynen, X Wei, B Tian, G Shi, G Cao, J Yuan, X Zhang
Cell Biosci, 2019;9(0):49.
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