Human/Mouse Phospho-RSK3 (S218) Antibody

Name: Human/Mouse Phospho-RSK3 (S218) Antibody
Brand: R&D Systems
SKU: AF893

Catalog #: AF893 Datasheet / COA / SDS

Discontinued Product

AF893 has been discontinued.

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Detection of Mouse Phospho-RSK3 (S218) by Western Blot.

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Human/Mouse Phospho-RSK3 (S218) Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse RSK3 when phosphorylated at S218. Reactivity with other RSK isoforms when phosphorylated at corresponding sites is unknown.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human and mouse RSK3 S218 site

Formulation

Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of mouse embryo (E13-15)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Mouse Phospho-RSK3 (S218) by Western Blot. Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120-HD) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-RSK3 (S218) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF893), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK3 (S218) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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Preparation and Storage

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Background: RSK3

The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.

Long Name

Ribosomal Protein S6 Kinase III

Entrez Gene IDs

6196 (Human); 20112 (Mouse)

Alternate Names

EC 2.7.11; EC 2.7.11.1; HU-2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK1C; MAPKAPK-1c; p90-RSK 2; p90RSK2; p90-RSK3; ribosomal protein S6 kinase alpha 2; ribosomal protein S6 kinase alpha-2; ribosomal protein S6 kinase, 90kD, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 2; Ribosomal S6 kinase 3,90 kDa ribosomal protein S6 kinase 2; RPS6KA2; RSK; RSK3; RSK-3; RSK3pp90RSK3; S6K-alpha; S6K-alpha2; S6K-alpha-2