Human/Mouse Phospho-RSK3 (S218) Antibody

  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects human and mouse RSK3 when phosphorylated at S218. Reactivity with other RSK isoforms when phosphorylated at corresponding sites is unknown.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human and mouse RSK3 S218 site
  • Formulation
    Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    Immersion fixed frozen sections of mouse embryo (E13-15)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of Mouse Phospho-RSK3 (S218) by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Human PDGF (Catalog # 120-HD) for for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human/Mouse Phospho-RSK3 (S218) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF893), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK3 (S218) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage
  • Shipping
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C, as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after opening.
    • 6 months, -20 to -70 °C under sterile conditions after opening.
Background: RSK3
The 90 kDa ribosomal protein S6 kinases (RSK1-4) are a family of broadly expressed Ser/Thr kinases activated in response to mitogenic stimuli, including growth factors and tumor-promoting phorbol esters. RSKs are directly phosphorylated by ERK1 and ERK2, leading to RSK autophosphorylation and activation. Active RSKs appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.
  • Long Name:
    Ribosomal Protein S6 Kinase III
  • Entrez Gene IDs:
    6196 (Human); 20112 (Mouse)
  • Alternate Names:
    EC 2.7.11; EC 2.7.11.1; HU-2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK1C; MAPKAPK-1c; p90-RSK 2; p90RSK2; p90-RSK3; ribosomal protein S6 kinase alpha 2; ribosomal protein S6 kinase alpha-2; ribosomal protein S6 kinase, 90kD, polypeptide 2; ribosomal protein S6 kinase, 90kDa, polypeptide 2; Ribosomal S6 kinase 3,90 kDa ribosomal protein S6 kinase 2; RPS6KA2; RSK; RSK3; RSK-3; RSK3pp90RSK3; S6K-alpha; S6K-alpha2; S6K-alpha-2
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