Human/Mouse PML Antibody
Human/Mouse PML Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human/Mouse PML by Western Blot. Western blot shows lysates of mouse immature thymus tissue and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse PML Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3799) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PML at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background:
The PML (promyelocytic leukemia) protein is a member of the tripartite motif (TRIM) family and contains multiple splice variants. PML nuclear splice variants localize to a subnuclear structure known as a PML-nuclear body (PML-NB), where it functions as a transcription factor and tumor suppressor. Several nuclear proteins localize to the PML-NB, including p53, p63, Daxx, CBP, and Sp100. Although cytoplasmic isoforms are less characterized, one isoform is a critical TGF-beta regulator. Additionally, cytoplasmic PML physically interacts with Smad2/3 and SARA, is required for the association of Smad2/3 with SARA and for the accumulation of SARA and TGF-beta receptor in the early endosome. A reciprocal translocation of PML located on chromosome 15, with the retinoic acid receptor-alpha (RAR alpha ) on chromosome 17 results in a PML/RAR alpha chimera found in virtually all cases of acute promyelocytic leukemia (APL).
Product Datasheets
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