Human/Mouse PRDM16/MEL1 Antibody

(8 citations)
(1 Review)
  
  • Species Reactivity
    Human, Mouse
  • Specificity
    Detects mouse and human PRDM16 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse PRDM16
    Lys537-Glu688
    Accession # A2A935.1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Immunohistochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PRDM16 by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PRDM16 at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Immunohistochemistry
PRDM16 in Mouse Embryo. PRDM16 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Sheep Anti-Human/Mouse PRDM16 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6295) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (orange, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to the trigeminal ganglion. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PRDM16/MEL1

PRDM16 (PR [PRDI-BF1 and RIZ] domain containing protein 16; also MEL-1) is a 170 kDa member of the PR Domain family of proteins. It is a transcriptional regulator expressed in the embryo, and is reported to participate in the maintenance of both neuronal and hematopoietic progenitor stem cells populations, and to preferentially promote the development of brown fat from adipomyocyte precursors. The generation of brown fat is likely due to suppression of muscle-specific factors. Mouse PRDM16 is 1275 amino acids (aa) in length. It contains one SET domain (aa 85-208) followed by ten C2H2 type Zn finger motifs (aa 230-1030). There are multiple potential isoform variants that likely vary from 150-170 kDa in size. One isoform shows a deletion of aa 1232-1250, a second isoform shows a three aa substitution for aa 1174-1275, and a third isoform possesses an alternative start site at Met21, coupled to a deletion of aa 1196-1133. Over aa 537-688, mouse PRDM16 shares 81% and 95% aa identity with human and rat PRDM16, respectively.

  • Long Name:
    PR Domain Containing 16
  • Entrez Gene IDs:
    63976 (Human); 70673 (Mouse)
  • Alternate Names:
    MDS1/EVI1-like gene 1; MEL1; MEL1KIAA1675PFM13MGC166915; PFM13; PR domain containing 16; PR domain zinc finger protein 16; PR domain-containing protein 16; PRDM16; Transcription factor MEL1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. Loss of ADAMTS5 enhances brown adipose tissue mass and promotes browning of white adipose tissue via CREB signaling
    Authors: D Bauters, M Cobbaut, L Geys, J Van Lint, B Hemmeryckx, HR Lijnen
    Mol Metab, 2017;6(7):715-724.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Transcription factor Hlx controls a systematic switch from white to brown fat through Prdm16-mediated co-activation
    Authors: L Huang, D Pan, Q Chen, LJ Zhu, J Ou, M Wabitsch, YX Wang
    Nat Commun, 2017;8(1):68.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: IP
  3. Fasting induces a subcutaneous-to-visceral fat switch mediated by microRNA-149-3p and suppression of PRDM16
    Authors: Hanying Ding
    Nat Commun, 2016;7(0):11533.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  4. White-to-brown metabolic conversion of human adipocytes by JAK inhibition.
    Authors: Moisan A, Lee Y, Zhang J, Hudak C, Meyer C, Prummer M, Zoffmann S, Truong H, Ebeling M, Kiialainen A, Gerard R, Xia F, Schinzel R, Amrein K, Cowan C
    Nat Cell Biol, 2015;17(1):57-67.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. MicroRNA-378 controls classical brown fat expansion to counteract obesity.
    Authors: Pan, Dongning, Mao, Chunxiao, Quattrochi, Brian, Friedline, Randall, Zhu, Lihua J, Jung, Dae Youn, Kim, Jason K, Lewis, Brian, Wang, Yong-Xu
    Nat Commun, 2014;5(0):4725.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  6. KSRP ablation enhances brown fat gene program in white adipose tissue through reduced miR-150 expression.
    Authors: Chou C, Lin Y, Wang H, Zhu X, Giovarelli M, Briata P, Gherzi R, Garvey W, Chen C
    Diabetes, 2014;63(9):2949-61.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  7. MyomiR-133 regulates brown fat differentiation through Prdm16.
    Authors: Trajkovski, Mirko, Ahmed, Kashan, Esau, Christin, Stoffel, Markus
    Nat Cell Biol, 2012;14(12):1330-5.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  8. Brown remodeling of white adipose tissue by SirT1-dependent deacetylation of Ppargamma.
    Authors: Qiang L, Wang L, Kon N, Zhao W, Lee S, Zhang Y, Rosenbaum M, Zhao Y, Gu W, Farmer S, Accili D
    Cell, 2012;150(3):620-32.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
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Immunohistochemistry Reagents
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Isotype Controls
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Normal Sheep IgG Control

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Secondary Antibodies
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Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

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Sheep IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Sheep IgG Biotinylated Antibody

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Sheep IgG (H+L) APC-conjugated Antibody

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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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Average Rating: 3 (Based on 1 review)

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We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Western Blot.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human/Mouse PRDM16/MEL1 Antibody AF6295
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 WB Mouse Anonymous 03/08/2016
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Western Blot Human/Mouse PRDM16/MEL1 Antibody AF6295
Western Blot: Human/Mouse PRDM16/MEL1 Antibody [AF6295]

Summary

ApplicationWestern Blot
Sample TestedAdipose tissue,Brown adipose tissue
SpeciesMouse

Other Experimental Details

Other Experimental DetailsSamples were nuclear extracts from brown adipose and subcutaneous adipose tissue (20ug protein loaded). Primary antibody (1:1,000) was detected with HRP-anti sheep (1:10,000) then amplified with Licor streptavidin 800 (1:10,000). Near infrared fluorescence gave much better signal-to-noise than chemiluminescence.

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