Home / PP2A / Human/Mouse/Rat Active PP2A DuoSet IC, 2 Plate

Human/Mouse/Rat Active PP2A DuoSet IC, 2 Plate

Catalog #: DYC3309-2
8 Citations Datasheet / COA / SDS

Discontinued Product

DYC3309-2 has been discontinued.
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Citations (8)
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Summary Product Features Kit Content Other Reagents Required Product Datasheets Preparation and Storage Background

Human/Mouse/Rat Active PP2A DuoSet IC, 2 Plate Summary

Assay Type
ELISA Development Kit for intracellular proteins
Assay Length
4 hours 40 mins (after plate preparation)
Sample Volume Required
Cell lysates (100 µL)
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15- (96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control
  • Streptavidin-HRP

Other Reagents Required


PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: From Costar EIA Plate (Costar Catalog # 2592) or R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

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Product Datasheet
COA
SDS

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PP2A

Protein Phosphatase 2A (PP2A) is an enzyme that removes phosphates covalently attached to serine and threonine residues in proteins. It contains three subunits; a catalytic subunit C (PP2Ac), an assembly/structural subunit A (PP2Aa), and a regulatory subunit B (PP2Ab). PP2A is a ubiquitous enzyme that plays a role in regulating many cellular activities varying from ion transport in erythrocytes to suppressing tumor growth. Binding of the SV40 small T antigen to the PP2A phosphatase complex is known to cause oncogenic transformation.

Entrez Gene IDs:
5515 (Human); 51792 (Mouse)
Alternate Names:
EC 3.1.3.16; PP2A; PP2A-alpha; PP2Ac; PP2CA; PP2Calpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2A catalytic subunit, alpha isoform; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform

Citations for Human/Mouse/Rat Active PP2A DuoSet IC, 2 Plate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Metformin alleviates lung-endothelial hyperpermeability by regulating cofilin-1/PP2AC pathway
    Authors: Siddiqui, MR;Reddy, NM;Faridi, HM;Shahid, M;Shanley, TP;
    Frontiers in pharmacology
    Species: Human
    Sample Types: Cell Lysates
  2. Arctigenin attenuates diabetic kidney disease through the activation of PP2A in podocytes
    Authors: Y Zhong, K Lee, Y Deng, Y Ma, Y Chen, X Li, C Wei, S Yang, T Wang, NJ Wong, AN Muwonge, EU Azeloglu, W Zhang, B Das, JC He, R Liu
    Nat Commun, 2019-10-04;10(1):4523.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Inhibition of PP2A activity by H2O2 during mitosis disrupts nuclear envelope reassembly and alters nuclear shape
    Authors: JH Ahn, MG Cho, S Sohn, JH Lee
    Exp. Mol. Med., 2019-06-05;51(6):64.
    Species: Human
    Sample Types: Cell Lysates
  4. Inhibition of AMP Kinase by the Protein Phosphatase 2A Heterotrimer, PP2APpp2r2d.
    Authors: Joseph B, Liu H, Francisco J, Pandya D, Donigan M, Gallo-Ebert C, Giordano C, Bata A, Nickels J
    2015-02-18;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
  5. cAMP signaling inhibits radiation-induced ATM phosphorylation leading to the augmentation of apoptosis in human lung cancer cells.
    Authors: Cho E, Kim E, Kwak S, Juhnn Y
    Mol Cancer, 2014-02-24;13(0):36.
    Species: Human
    Sample Types: Cell Lysates
  6. Targeting energy metabolic and oncogenic signaling pathways in triple-negative breast cancer by a novel adenosine monophosphate-activated protein kinase (AMPK) activator.
    Authors: Lee KH, Hsu EC, Guh JH, Yang HC, Wang D, Kulp SK, Shapiro CL, Chen CS
    J. Biol. Chem., 2011-09-14;286(45):39247-58.
    Species: Human
    Sample Types: Cell Lysates
  7. Folate is related to phosphorylated neurofilament-H and P-tau (Ser396) in rat brain.
    Authors: Obeid R, Schlundt J, Umanskaya N, Herrmann W, Herrmann M
    J. Neurochem., 2011-05-13;117(6):1047-54.
    Species: Rat
    Sample Types: Tissue Homogenates
  8. The B56gamma3 regulatory subunit of protein phosphatase 2A (PP2A) regulates S phase-specific nuclear accumulation of PP2A and the G1 to S transition.
    Authors: Lee TY, Lai TY, Lin SC
    J. Biol. Chem., 2010-05-06;285(28):21567-80.
    Species: Mouse
    Sample Types: Cell Lysates

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