Human/Mouse/Rat Active SHP-1 DuoSet IC, 1 Plate

Name: Human/Mouse/Rat Active SHP-1 DuoSet IC, 1 Plate
Brand: R&D Systems
SKU: DYC2808

Catalog #: DYC2808
2 Citations Datasheet / COA / SDS

Discontinued Product

DYC2808 has been discontinued.

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Product Details

Citations (2)

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Summary Product Features Kit Content Other Reagents Required Product Datasheets Preparation and Storage Background

Human/Mouse/Rat Active SHP-1 DuoSet IC, 1 Plate Summary

Assay Type

Immunoprecipitation Assay Development Kit

Assay Length

4 hours 40 minutes (after plate preparation)

Sample Volume Required

100 µL

Sufficient Materials

For one 96-well plate*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Active SHP-1 Immunoprecipitation Beads
Tyrosine Phosphatase Substrate I
Phosphate Standard
Malachite Green Reagent A
Malachite Green Reagent B

Other Reagents Required

TBS: 25 mM Tris, 150 mM NaCl, pH 7.5.

IC Diluent #10: 50 mM HEPES, 0.1 mM EGTA, 0.1 mM EDTA, 120 mM NaCl, 0.5% NP-40 Alternative, pH 7.5. Store at 2 - 8° C for up to 3 months.

IC Diluent #11: 10 mM HEPES, 0.1 mM EGTA, 0.1 mM EDTA, 0.5% BSA*, 1 mM DTT, pH 7.5. Prepare fresh just before use.

Note: The presence of DTT is essential to measure phosphatase activity.

Lysis Buffer #8: 50 mM HEPES, 0.1 mM EGTA, 0.1 mM EDTA, 120 mM NaCl, 0.5% NP-40 Alternative (pH 7.5), 25 µg/mL Leupeptin, 25 µg/mL Pepstatin, 2 µg/mL Aprotinin, 1 mM PMSF. Prepare fresh just before use.

Note: Lysis Buffer #8 consists of IC Diluent #10 plus 25 µg/mL Leupeptin, 25 µg/mL Pepstatin, 2 µg/mL Aprotinin, and 1 mM PMSF. Approximately 50 mL of IC Diluent #10 is required to run the assay on one 96 well plate.

*The use of Millpore Bovine Serum Albumin, Fraction V, Protease free (Catalog # 82-045) is recommended. All buffers containing BSA must be stored at 2 - 8° C.

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: SHP-1

The SH2 domain-containing phosphatases, SHP-1 and SHP-2, are well-studied cytosolic tyrosine phosphatases that share many structural and regulatory features. They both have two tandem SH2 domains at the N-terminus, followed by a catalytic domain, and an inhibitory C-terminus. Despite their structural similarity, the enzymes appear to have different physiological roles and exhibit different expression patterns. SHP-1 is highly expressed in hematopoietic tissues, and in general has a negative impact on lymphocyte signaling. In contrast, SHP-2 is widely expressed, and in general promotes signaling pathways that lead to differentiation, cell growth, or migration.

Long Name:

Src Homology Region 2 Domain Phosphatase 1

Entrez Gene IDs:

5777 (Human); 15170 (Mouse); 116689 (Rat)

Alternate Names:

HCP; HCPH; HPTP1C; PTP1C; PTP-1C; PTPN6; SHP1; SHP-1; SHP-1L; SH-PTP1

Citations for Human/Mouse/Rat Active SHP-1 DuoSet IC, 1 Plate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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