Human/Mouse/Rat Activin A Quantikine ELISA Kit

(18 citations)
(4 Reviews)
  
  • Assay Length
    5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
  • Sensitivity
    7.85 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human, mouse, and rat Activin A
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC33, Quantikine Immunoassay Control Set 586 for H/M/R Activin A - Please Inquire
Product Summary
The Quantikine Human/Mouse/Rat Activin A Immunoassay is a 5.0 hour solid-phase ELISA designed to measure Activin A in human, mouse, or rat cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human/mouse/rat Activin A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/mouse/rat Activin A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed Activin A.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean101295498106308529
Standard Deviation4.212.521.98.415.524.8
CV%4.24.24.47.954.7

Recovery

The recovery of human Activin A spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 93-114
EDTA Plasma (n=4) 110 95-115
Heparin Plasma (n=4) 107 99-113
Serum (n=4) 109 100-115
Linearity
To assess the linearity of the assay, human samples containing and/or spiked with high concentrations of Activin A were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human/Mouse/Rat Activin A Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Activin A

Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Activin proteins have a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and roles in reproductive physiology. Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction.

Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned to date. The activin/inhibin nomenclature reflects the subunit composition of the proteins: Activin A (beta A - beta A), Activin B (beta B - beta B), Activin AB (beta A - beta B), Inhibin A (alpha - beta A) and Inhibin B (alpha - beta B).

    • Entrez Gene IDs
      3624 (Human); 16323 (Mouse);
    • Alternate Names
      Activin A; INHBA;
    Related Research Areas
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    18 Citations: Showing 1 - 10
    Filter your results:

    Species
    Sample Type
    1. Activin A more prominently regulates muscle mass in primates than does GDF8
      Authors: E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada
      Nat Commun, 2017;8(0):15153.
      Species: Human
      Sample Type: Serum
    2. Activin A Inhibits MPTP and LPS-Induced Increases in Inflammatory Cell Populations and Loss of Dopamine Neurons in the Mouse Midbrain In Vivo
      Authors: S Stayte, P Rentsch, AR Tr”scher, M Bamberger, KM Li, B Vissel
      PLoS ONE, 2017;12(1):e0167211.
      Species: Mouse
      Sample Type: Tissue Homogenates
    3. Vitamin D supplementation lowers thrombospondin-1 levels and blood pressure in healthy adults
      Authors: AT Amaraseker, B Assadi-Kha, S Liu, M Black, G Dymmott, NM Rogers, AL Sverdlov, JD Horowitz, DTM Ngo
      PLoS ONE, 2017;12(5):e0174435.
      Species: Human
      Sample Type: Plasma
    4. Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
      Authors: F Sallustio, C Curci, A Aloisi, CC Toma, E Marulli, G Serino, SN Cox, G De Palma, A Stasi, C Divella, R Rinaldi, FP Schena
      Sci Rep, 2017;7(1):8225.
      Species: Human
      Sample Type: Whole Cells
    5. Activin A is increased in the nucleus accumbens following a cocaine binge
      Authors: ZJ Wang, JA Martin, AM Gancarz, DN Adank, FJ Sim, DM Dietz
      Sci Rep, 2017;7(0):43658.
      Species: Rat
      Sample Type: Tissue Homogenates
    6. Immunoselected STRO-3(+) mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis
      Authors: A Abdalmula, LM Dooley, C Kaufman, EA Washington, JV House, BA Blacklaws, P Ghosh, S Itescu, SR Bailey, WG Kimpton
      Stem Cell Res Ther, 2017;8(1):22.
      Species: Ovine
      Sample Type: Plasma
    7. A Key Role for Inhibins in Dendritic Cell Maturation and Function
      PLoS ONE, 2016;11(12):e0167813.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    8. Late acute graft versus host disease: a prospective analysis of clinical outcomes and circulating angiogenic factors
      Blood, 2016;0(0):.
      Species: Human
      Sample Type: Plasma
    9. Activin A programs the differentiation of human TFH cells
      Nat Immunol, 2016;17(8):976-84.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta.
      Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F
      PLoS ONE, 2015;10(2):e0118097.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. The differential anti-tumour effects of zoledronic acid in breast cancer - evidence for a role of the activin signaling pathway.
      Authors: Wilson C, Ottewell P, Coleman R, Holen I
      BMC Cancer, 2015;15(0):55.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Serum activin A and follistatin levels in gestational diabetes and the association of the Activin A-Follistatin system with anthropometric parameters in offspring.
      Authors: Naf S, Escote X, Ballesteros M, Yanez R, Simon-Muela I, Gil P, Albaiges G, Vendrell J, Megia A
      PLoS ONE, 2014;9(4):e92175.
      Species: Human
      Sample Type: Serum
    13. PMA induces SnoN proteolysis and CD61 expression through an autocrine mechanism.
      Authors: Li C, Peart N, Xuan Z, Lewis D, Xia Y, Jin J
      Cell Signal, 2014;26(7):1369-78.
      Species: Human
      Sample Type: Cell Culture Supernates
    14. Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells.
      Authors: Chen L, Zhang W, Liang H, Zhou Q, Ding Z, Yang H, Liu W, Wu Y, Man Q, Zhang B, Chen X
      Cell Commun Signal, 2014;12(0):18.
      Species: Rat
      Sample Type: Cell Culture Supernates
    15. An essential role for insulin and IGF1 receptors in regulating sertoli cell proliferation, testis size, and FSH action in mice.
      Authors: Pitetti J, Calvel P, Zimmermann C, Conne B, Papaioannou M, Aubry F, Cederroth C, Urner F, Fumel B, Crausaz M, Docquier M, Herrera P, Pralong F, Germond M, Guillou F, Jegou B, Nef S
      Mol Endocrinol, 2013;27(5):814-27.
      Species: Mouse
      Sample Type: Serum
    16. Activin A is associated with impaired myocardial glucose metabolism and left ventricular remodeling in patients with uncomplicated type 2 diabetes.
      Authors: Chen W, Greulich S, van der Meer R, Rijzewijk L, Lamb H, de Roos A, Smit J, Romijn J, Ruige J, Lammertsma A, Lubberink M, Diamant M, Ouwens D
      Cardiovasc Diabetol, 2013;12(1):150.
      Species: Human
      Sample Type: Plasma
    17. Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers.
      Authors: Sierra-Filardi E, Puig-Kroger A, Blanco FJ, Nieto C, Bragado R, Palomero MI, Bernabeu C, Vega MA, Corbi AL
      Blood, 2011;117(19):5092-101.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    18. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
      Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
      J. Bone Miner. Res., 2011;26(8):1891-903.
      Species: Human
      Sample Type: Serum
    Expand to show all 18 Citations
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 586 for H/M/R Activin A

    Ctrl QC33
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
    View Sizes & Prices
    Catalog# Size Price Stock Quantity
    Loading Information....
    Average Rating: 4.3 (Based on 4 reviews)

    Have you used Human/Mouse/Rat Activin A Quantikine ELISA Kit?

    Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

    Read what people are saying who have used Human/Mouse/Rat Activin A Quantikine ELISA Kit.

    Filter by Ratings
    Excellent
    (1)
    Very Good
    (3)
    All Ratings
    Filter by Applications
    All Applications
    Filter by Species
    All Species

    Images Ratings Applications Species Reviewed By Date Details
      Excellent
      Anonymous 05/16/2017
    Show

    Summary

    Sample TestedRecombinant protein
      Very Good
      Anonymous 05/03/2017
    Show

    Summary

    Sample TestedSerum
      Very Good
      Anonymous 04/24/2017
    Show

    Summary

    Sample TestedSerum
      Very Good
      Anonymous 04/21/2017
    Show

    Summary

    Sample TestedRecombinant protein

    Order Details

    Contact Information

    R&D Systems Guarantee

    Feedback

    Customer Information