Human/Mouse/Rat Activin A Quantikine ELISA Kit

(4 citations)   
  • Assay Length
    5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
  • Sensitivity
    7.85 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human, mouse, and rat Activin A
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
Product Summary
The Quantikine Human/Mouse/Rat Activin A Immunoassay is a 5.0 hour solid-phase ELISA designed to measure Activin A in human, mouse, or rat cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human/mouse/rat Activin A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/mouse/rat Activin A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed Activin A.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Standard Deviation4.212.521.98.415.524.8


The recovery of human Activin A spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 93-114
EDTA Plasma (n=4) 110 95-115
Heparin Plasma (n=4) 107 99-113
Serum (n=4) 109 100-115
To assess the linearity of the assay, human samples containing and/or spiked with high concentrations of Activin A were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human/Mouse/Rat Activin A Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Activin A

Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Activin proteins have a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and roles in reproductive physiology. Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction.

Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned to date. The activin/inhibin nomenclature reflects the subunit composition of the proteins: Activin A (beta A - beta A), Activin B (beta B - beta B), Activin AB (beta A - beta B), Inhibin A (alpha - beta A) and Inhibin B (alpha - beta B).

    • Entrez Gene IDs
      3624 (Human); 16323 (Mouse);
    • Alternate Names
    Related Research Areas

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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    Sample Type
    1. The differential anti-tumour effects of zoledronic acid in breast cancer - evidence for a role of the activin signaling pathway.
      Authors: Wilson C, Ottewell P, Coleman R, Holen I
      BMC Cancer, 2015;15(0):55.
      Species: Human
      Sample Type: cell culture supernate
    2. Activin A is associated with impaired myocardial glucose metabolism and left ventricular remodeling in patients with uncomplicated type 2 diabetes.
      Authors: Chen W, Greulich S, van der Meer R, Rijzewijk L, Lamb H, de Roos A, Smit J, Romijn J, Ruige J, Lammertsma A, Lubberink M, Diamant M, Ouwens D
      Cardiovasc Diabetol, 2013;12(1):150.
      Species: Human
      Sample Type: Plasma
    3. Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers.
      Authors: Sierra-Filardi E, Puig-Kroger A, Blanco FJ, Nieto C, Bragado R, Palomero MI, Bernabeu C, Vega MA, Corbi AL
      Blood, 2011;117(19):5092-101.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    4. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
      Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
      J. Bone Miner. Res., 2011;26(8):1891-903.
      Species: Human
      Sample Type: Serum
    ELISA Controls
    Description Application Cat# Citations Images  

    Quantikine Immunoassay Control Set 586 for H/M/R Activin A

    Ctrl QC33
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    Supplemental ELISA Products
    Description Application Cat# Citations Images  

    Quantikine Wash Buffer 1

    ELISA WA126 5
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