Human/Mouse/Rat Activin A Quantikine ELISA Kit

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DAC00B
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Human/Mouse/Rat Activin A ELISA Standard Curve
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Product Details
Citations (39)
FAQs
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Reviews (8)

Human/Mouse/Rat Activin A Quantikine ELISA Kit Summary

Assay Length
5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
Sensitivity
7.85 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
Specificity
Natural and recombinant human, mouse, and rat Activin A
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human/Mouse/Rat Activin A Immunoassay is a 5.0 hour solid-phase ELISA designed to measure Activin A in human, mouse, or rat cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human/mouse/rat Activin A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/mouse/rat Activin A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed Activin A.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 101 295 498 106 308 529
Standard Deviation 4.2 12.5 21.9 8.4 15.5 24.8
CV% 4.2 4.2 4.4 7.9 5 4.7

Recovery

The recovery of human Activin A spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 93-114
EDTA Plasma (n=4) 110 95-115
Heparin Plasma (n=4) 107 99-113
Serum (n=4) 109 100-115

Linearity

To assess the linearity of the assay, human samples containing and/or spiked with high concentrations of Activin A were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human/Mouse/Rat Activin A ELISA Linearity

Data Examples

Human/Mouse/Rat Activin A ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Activin A

Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Activin proteins have a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and roles in reproductive physiology. Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction.

Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned to date. The activin/inhibin nomenclature reflects the subunit composition of the proteins: Activin A (beta A - beta A), Activin B (beta B - beta B), Activin AB (beta A - beta B), Inhibin A (alpha - beta A) and Inhibin B (alpha - beta B).

Entrez Gene IDs:
3624 (Human); 16323 (Mouse); 29200 (Rat)
Alternate Names:
Activin A; activin AB alpha polypeptide; Activin beta-A chain; erythroid differentiation factor; Erythroid differentiation protein; follicle-stimulating hormone-releasing protein; FSH-releasing protein; inhibin beta A chain; inhibin beta A subunit; Inhibin, beta-1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human/Mouse/Rat Activin A Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Activin A Modulates Inflammation in Acute Pancreatitis and Strongly Predicts Severe Disease Independent of Body Mass Index
    Authors: AL Thomas, K Castellano, G Mancinelli, Y Xia, J Bauer, C Yazici, G Fantuzzi, RF Hwang, NL Krett, GI Papachrist, DC Whitcomb, B Jung
    Clin Transl Gastroenterol, 2020;11(5):e00152.
    Species: Mouse
    Sample Types: Plasma
  2. Increased stiffness of the tumor microenvironment in colon cancer stimulates cancer associated fibroblast-mediated prometastatic activin A signaling
    Authors: J Bauer, MAB Emon, JJ Staudacher, AL Thomas, J Zessner-Sp, G Mancinelli, N Krett, MT Saif, B Jung
    Sci Rep, 2020;10(1):50.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Microphthalmia transcription factor expression contributes to bone marrow failure in Fanconi anemia
    Authors: A Oppezzo, J Bourseguin, E Renaud, P Pawlikowsk, F Rosselli
    J. Clin. Invest., 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Urinary Activin A is a novel biomarker reflecting renal inflammation and tubular damage in ANCA-associated vasculitis
    Authors: Y Takei, S Takahashi, M Nakasatomi, T Sakairi, H Ikeuchi, Y Kaneko, K Hiromura, Y Nojima, A Maeshima
    PLoS ONE, 2019;14(10):e0223703.
    Species: Human
    Sample Types: Serum
  5. Activin A regulates the epidermal growth factor receptor promoter by activating the PI3K/SP1 pathway in oral squamous cell carcinoma cells
    Authors: CN Tsai, CL Tsai, JS Yi, HK Kao, Y Huang, CI Wang, YS Lee, KP Chang
    Sci Rep, 2019;9(1):5197.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Blockade of ALK4/5 signaling suppresses cadmium- and erastin-induced cell death in renal proximal tubular epithelial cells via distinct signaling mechanisms
    Authors: K Fujiki, H Inamura, T Sugaya, M Matsuoka
    Cell Death Differ., 2019;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. Time dependent degeneration of the nigrostriatal tract in mice with 6-OHDA lesioned medial forebrain bundle and the effect of activin A on L-Dopa induced dyskinesia
    Authors: P Rentsch, S Stayte, GP Morris, B Vissel
    BMC Neurosci, 2019;20(1):5.
    Species: Mouse
    Sample Types: Tissue Homogenates
  8. Senolytics in idiopathic pulmonary fibrosis: Results from a first-in-human, open-label, pilot study
    Authors: JN Justice, AM Nambiar, T Tchkonia, NK LeBrasseur, R Pascual, SK Hashmi, L Prata, MM Masternak, SB Kritchevsk, N Musi, JL Kirkland
    EBioMedicine, 2019;0(0):.
    Species: Human
    Sample Types: Serum
  9. Activin A inhibition attenuates sympathetic neural remodeling following myocardial infarction in rats
    Authors: J Hu, X Wang, YH Tang, YG Shan, Q Zou, ZQ Wang, CX Huang
    Mol Med Rep, 2018;17(4):5074-5080.
    Species: Rat
    Sample Types: Serum
  10. NF-?B/MAPK activation underlies ACVR1-mediated inflammation in human heterotopic ossification
    Authors: E Barruet, BM Morales, CJ Cain, AN Ton, KL Wentworth, TV Chan, TA Moody, MC Haks, TH Ottenhoff, J Hellman, MC Nakamura, EC Hsiao
    JCI Insight, 2018;3(22):.
    Species: Human
    Sample Types: Serum
  11. Activin subfamily peptides predict chronological age in humans
    Authors: LV Barrios-Si, M Parnell, ZB Shinwari, GA Chaudhary, T Xenofontos, A van Bekhov, S McArthur, BT Elliott
    Physiol Rep, 2018;6(17):e13823.
    Species: Human
    Sample Types: Plasma
  12. Stroma-derived IL-6, G-CSF and Activin-A mediated dedifferentiation of lung carcinoma cells into cancer stem cells
    Authors: CFD Rodrigues, E Serrano, MI Patrício, MM Val, P Albuquerqu, J Fonseca, CMF Gomes, AJ Abrunhosa, A Paiva, L Carvalho, MF Botelho, L Almeida, IM Carreira, MC Alpoim
    Sci Rep, 2018;8(1):11573.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells
    Authors: M Shoji, H Minato, S Ogaki, M Seki, Y Suzuki, S Kume, T Kuzuhara
    PLoS ONE, 2018;13(7):e0201239.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  14. Activin A is increased in the nucleus accumbens following a cocaine binge
    Authors: ZJ Wang, JA Martin, AM Gancarz, DN Adank, FJ Sim, DM Dietz
    Sci Rep, 2017;7(0):43658.
    Species: Rat
    Sample Types: Tissue Homogenates
  15. Activin in acute pancreatitis: Potential risk-stratifying marker and novel therapeutic target.
    Authors: Jonas J Staudacher, Cemal Yazici, Timothy Carroll, Jessica Bauer, Jingbo Pang, Nancy Krett, Yinglin Xia, Annette Wilson, Georgios Papachris, Andrea Dirmeier, Claudia Kunst, David C Whitcomb, Giamila Fantuzzi, Barbara Jung
    Scientific Reports, 2017;0(0):2045-2322.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Capture
  16. ACVR2B/Fc counteracts chemotherapy-induced loss of muscle and bone mass
    Authors: R Barreto, Y Kitase, T Matsumoto, F Pin, KC Colston, KE Couch, TM O'Connell, ME Couch, LF Bonewald, A Bonetto
    Sci Rep, 2017;7(1):14470.
    Species: Mouse
    Sample Types: Plasma
  17. Immunoselected STRO-3(+) mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis
    Authors: A Abdalmula, LM Dooley, C Kaufman, EA Washington, JV House, BA Blacklaws, P Ghosh, S Itescu, SR Bailey, WG Kimpton
    Stem Cell Res Ther, 2017;8(1):22.
    Species: Ovine
    Sample Types: Plasma
  18. Trace element and cytokine concentrations in patients with Fibrodysplasia Ossificans Progressiva (FOP): A case control study
    Authors: Laura Hildebrand
    J Trace Elem Med Biol, 2017;39(0):186-192.
    Species: Human
    Sample Types: Serum
  19. Vitamin D supplementation lowers thrombospondin-1 levels and blood pressure in healthy adults
    Authors: AT Amaraseker, B Assadi-Kha, S Liu, M Black, G Dymmott, NM Rogers, AL Sverdlov, JD Horowitz, DTM Ngo
    PLoS ONE, 2017;12(5):e0174435.
    Species: Human
    Sample Types: Plasma
  20. Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
    Authors: F Sallustio, C Curci, A Aloisi, CC Toma, E Marulli, G Serino, SN Cox, G De Palma, A Stasi, C Divella, R Rinaldi, FP Schena
    Sci Rep, 2017;7(1):8225.
    Species: Human
    Sample Types: Whole Cells
  21. Activin A more prominently regulates muscle mass in primates than does GDF8
    Authors: E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada
    Nat Commun, 2017;8(0):15153.
    Species: Human
    Sample Types: Serum
  22. Activin A Inhibits MPTP and LPS-Induced Increases in Inflammatory Cell Populations and Loss of Dopamine Neurons in the Mouse Midbrain In Vivo
    Authors: S Stayte, P Rentsch, AR Tr”scher, M Bamberger, KM Li, B Vissel
    PLoS ONE, 2017;12(1):e0167211.
    Species: Mouse
    Sample Types: Tissue Homogenates
  23. Late acute graft versus host disease: a prospective analysis of clinical outcomes and circulating angiogenic factors
    Blood, 2016;0(0):.
    Species: Human
    Sample Types: Plasma
  24. Activin A programs the differentiation of human TFH cells
    Nat Immunol, 2016;17(8):976-84.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Diet-induced weight loss is sufficient to reduce senescent cell number in white adipose tissue of weight-cycled mice
    Authors: Edward O List
    Nutr Healthy Aging, 2016;4(1):95-99.
    Species: Mouse
    Sample Types: Plasma
  26. Predicting first trimester pregnancy outcome: derivation of a multiple marker test
    Authors: S Senapati, MD Sammel, SF Butts, P Takacs, K Chung, KT Barnhart
    Fertil. Steril., 2016;106(7):1725-1732.e3.
    Species: Human
    Sample Types: Serum
  27. Involvement of Infiltrating Macrophage-derived Activin A in the Progression of Renal Damage in MRL-lpr Mice
    Authors: Yoshihisa Nojima
    Am. J. Physiol. Renal Physiol., 2016;0(0):ajprenal.0019.
    Species: Mouse
    Sample Types: Urine
  28. A Key Role for Inhibins in Dendritic Cell Maturation and Function
    PLoS ONE, 2016;11(12):e0167813.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  29. Acute Molecular Changes in Synovial Fluid Following Human Knee Injury: Association With Early Clinical Outcomes
    Authors: Tonia L Vincent
    Arthritis & rheumatology (Hoboken, N.J.), 2016;68(9):2129-40.
  30. The differential anti-tumour effects of zoledronic acid in breast cancer - evidence for a role of the activin signaling pathway.
    Authors: Wilson C, Ottewell P, Coleman R, Holen I
    BMC Cancer, 2015;15(0):55.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta.
    Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F
    PLoS ONE, 2015;10(2):e0118097.
    Species: Human
    Sample Types: Cell Culture Supernates
  32. Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells.
    Authors: Chen L, Zhang W, Liang H, Zhou Q, Ding Z, Yang H, Liu W, Wu Y, Man Q, Zhang B, Chen X
    Cell Commun Signal, 2014;12(0):18.
    Species: Rat
    Sample Types: Cell Culture Supernates
  33. PMA induces SnoN proteolysis and CD61 expression through an autocrine mechanism.
    Authors: Li C, Peart N, Xuan Z, Lewis D, Xia Y, Jin J
    Cell Signal, 2014;26(7):1369-78.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Serum activin A and follistatin levels in gestational diabetes and the association of the Activin A-Follistatin system with anthropometric parameters in offspring.
    Authors: Naf S, Escote X, Ballesteros M, Yanez R, Simon-Muela I, Gil P, Albaiges G, Vendrell J, Megia A
    PLoS ONE, 2014;9(4):e92175.
    Species: Human
    Sample Types: Serum
  35. Activin A is associated with impaired myocardial glucose metabolism and left ventricular remodeling in patients with uncomplicated type 2 diabetes.
    Authors: Chen W, Greulich S, van der Meer R, Rijzewijk L, Lamb H, de Roos A, Smit J, Romijn J, Ruige J, Lammertsma A, Lubberink M, Diamant M, Ouwens D
    Cardiovasc Diabetol, 2013;12(1):150.
    Species: Human
    Sample Types: Plasma
  36. An essential role for insulin and IGF1 receptors in regulating sertoli cell proliferation, testis size, and FSH action in mice.
    Authors: Pitetti J, Calvel P, Zimmermann C, Conne B, Papaioannou M, Aubry F, Cederroth C, Urner F, Fumel B, Crausaz M, Docquier M, Herrera P, Pralong F, Germond M, Guillou F, Jegou B, Nef S
    Mol Endocrinol, 2013;27(5):814-27.
    Species: Mouse
    Sample Types: Serum
  37. Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers.
    Authors: Sierra-Filardi E, Puig-Kroger A, Blanco FJ, Nieto C, Bragado R, Palomero MI, Bernabeu C, Vega MA, Corbi AL
    Blood, 2011;117(19):5092-101.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  38. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
    Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
    J. Bone Miner. Res., 2011;26(8):1891-903.
    Species: Human
    Sample Types: Serum
  39. Activin subfamily peptides predict chronological age in humans.
    Authors: Barrios-Silva L, Parnell M, Shinwari Z, Chaudhary G, Xenofontos T, van Bekhoven A, McArthur S, Elliott B
    Physiol Rep, 0;6(17):e13823.
    Species: Human
    Sample Types: Plasma

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Reviews for Human/Mouse/Rat Activin A Quantikine ELISA Kit

Average Rating: 4.6 (Based on 8 Reviews)

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Human/Mouse/Rat Activin A Quantikine ELISA Kit
By Anonymous on 11/06/2020
Application: Sample Tested: Cell culture supernatant

Human/Mouse/Rat Activin A Quantikine ELISA Kit
By Anonymous on 11/04/2020
Application: Sample Tested: Cell culture supernatant

Human/Mouse/Rat Activin A Quantikine ELISA Kit
By Anonymous on 06/26/2020
Application: Sample Tested: Serum-free Cell Culture Media,T cells

reliable product and easy to use


Human/Mouse/Rat Activin A Quantikine ELISA Kit
By Anonymous on 03/14/2019
Application: Sample Tested: Serum and Plasma

Human/Mouse/Rat Activin A Quantikine ELISA Kit
By Anonymous on 05/16/2017
Application: Sample Tested: Recombinant protein

Human/Mouse/Rat Activin A Quantikine ELISA Kit
By Anonymous on 05/03/2017
Application: Sample Tested: Serum

Human/Mouse/Rat Activin A Quantikine ELISA Kit
By Anonymous on 04/24/2017
Application: Sample Tested: Serum

Human/Mouse/Rat Activin A Quantikine ELISA Kit
By Anonymous on 04/21/2017
Application: Sample Tested: Recombinant protein