Human/Mouse/Rat Activin A Quantikine ELISA Kit

  (23 citations)
(4 Reviews)
    
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Citations (23)
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  • Assay Length
    5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)
  • Sensitivity
    7.85 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva)
  • Specificity
    Natural and recombinant human, mouse, and rat Activin A
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC33 , Quantikine Immunoassay Control Set 586 for H/M/R Activin A - Please Inquire
Product Summary
The Quantikine Human/Mouse/Rat Activin A Immunoassay is a 5.0 hour solid-phase ELISA designed to measure Activin A in human, mouse, or rat cell culture supernates, serum, plasma, and saliva. It contains CHO cell-expressed recombinant human/mouse/rat Activin A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human/mouse/rat Activin A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally expressed Activin A.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 101 295 498 106 308 529
Standard Deviation 4.2 12.5 21.9 8.4 15.5 24.8
CV% 4.2 4.2 4.4 7.9 5 4.7

Recovery

The recovery of human Activin A spiked to three different levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 93-114
EDTA Plasma (n=4) 110 95-115
Heparin Plasma (n=4) 107 99-113
Serum (n=4) 109 100-115
Linearity
To assess the linearity of the assay, human samples containing and/or spiked with high concentrations of Activin A were diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Activin A [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Activin A

Activins, members of the TGF-beta superfamily, are disulfide-linked dimeric proteins originally purified from gonadal fluids as proteins that stimulated pituitary follicle stimulating hormone (FSH) release. Activin proteins have a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodeling, hematopoiesis and roles in reproductive physiology. Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction.

Activins are homodimers or heterodimers of the various beta subunit isoforms, while inhibins are heterodimers of a unique alpha subunit and one of the various beta subunits. Five beta subunits (mammalian beta A, beta B, beta C, beta E and Xenopus beta D) have been cloned to date. The activin/inhibin nomenclature reflects the subunit composition of the proteins: Activin A (beta A - beta A), Activin B (beta B - beta B), Activin AB (beta A - beta B), Inhibin A (alpha - beta A) and Inhibin B (alpha - beta B).

  • Entrez Gene IDs:
    3624 (Human); 16323 (Mouse)
  • Alternate Names:
    Activin A; INHBA
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

23 Citations: Showing 1 - 10
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Species
Sample Type
  1. Activin A inhibition attenuates sympathetic neural remodeling following myocardial infarction in rats
    Authors: J Hu, X Wang, YH Tang, YG Shan, Q Zou, ZQ Wang, CX Huang
    Mol Med Rep, 2018;17(4):5074-5080.
    Species: Rat
    Sample Type: Serum
  2. Activin A Inhibits MPTP and LPS-Induced Increases in Inflammatory Cell Populations and Loss of Dopamine Neurons in the Mouse Midbrain In Vivo
    Authors: S Stayte, P Rentsch, AR Tr”scher, M Bamberger, KM Li, B Vissel
    PLoS ONE, 2017;12(1):e0167211.
    Species: Mouse
    Sample Type: Tissue Homogenates
  3. Vitamin D supplementation lowers thrombospondin-1 levels and blood pressure in healthy adults
    Authors: AT Amaraseker, B Assadi-Kha, S Liu, M Black, G Dymmott, NM Rogers, AL Sverdlov, JD Horowitz, DTM Ngo
    PLoS ONE, 2017;12(5):e0174435.
    Species: Human
    Sample Type: Plasma
  4. Immunoselected STRO-3(+) mesenchymal precursor cells reduce inflammation and improve clinical outcomes in a large animal model of monoarthritis
    Authors: A Abdalmula, LM Dooley, C Kaufman, EA Washington, JV House, BA Blacklaws, P Ghosh, S Itescu, SR Bailey, WG Kimpton
    Stem Cell Res Ther, 2017;8(1):22.
    Species: Ovine
    Sample Type: Plasma
  5. Trace element and cytokine concentrations in patients with Fibrodysplasia Ossificans Progressiva (FOP): A case control study
    Authors: Laura Hildebrand
    J Trace Elem Med Biol, 2017;39(0):186-192.
    Species: Human
    Sample Type: Serum
  6. ACVR2B/Fc counteracts chemotherapy-induced loss of muscle and bone mass
    Authors: R Barreto, Y Kitase, T Matsumoto, F Pin, KC Colston, KE Couch, TM O'Connell, ME Couch, LF Bonewald, A Bonetto
    Sci Rep, 2017;7(1):14470.
    Species: Mouse
    Sample Type: Plasma
  7. Activin A more prominently regulates muscle mass in primates than does GDF8
    Authors: E Latres, J Mastaitis, W Fury, L Miloscio, J Trejos, J Pangilinan, H Okamoto, K Cavino, E Na, A Papatheodo, T Willer, Y Bai, J Hae Kim, A Rafique, S Jaspers, T Stitt, AJ Murphy, GD Yancopoulo, J Gromada
    Nat Commun, 2017;8(0):15153.
    Species: Human
    Sample Type: Serum
  8. Activin A is increased in the nucleus accumbens following a cocaine binge
    Authors: ZJ Wang, JA Martin, AM Gancarz, DN Adank, FJ Sim, DM Dietz
    Sci Rep, 2017;7(0):43658.
    Species: Rat
    Sample Type: Tissue Homogenates
  9. Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration
    Authors: F Sallustio, C Curci, A Aloisi, CC Toma, E Marulli, G Serino, SN Cox, G De Palma, A Stasi, C Divella, R Rinaldi, FP Schena
    Sci Rep, 2017;7(1):8225.
    Species: Human
    Sample Type: Whole Cells
  10. Predicting first trimester pregnancy outcome: derivation of a multiple marker test
    Authors: S Senapati, MD Sammel, SF Butts, P Takacs, K Chung, KT Barnhart
    Fertil. Steril., 2016;106(7):1725-1732.e3.
    Species: Human
    Sample Type: Serum
  11. Diet-induced weight loss is sufficient to reduce senescent cell number in white adipose tissue of weight-cycled mice
    Authors: Edward O List
    Nutr Healthy Aging, 2016;4(1):95-99.
    Species: Mouse
    Sample Type: Plasma
  12. Activin A programs the differentiation of human TFH cells
    Nat Immunol, 2016;17(8):976-84.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. Late acute graft versus host disease: a prospective analysis of clinical outcomes and circulating angiogenic factors
    Blood, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  14. A Key Role for Inhibins in Dendritic Cell Maturation and Function
    PLoS ONE, 2016;11(12):e0167813.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  15. The differential anti-tumour effects of zoledronic acid in breast cancer - evidence for a role of the activin signaling pathway.
    Authors: Wilson C, Ottewell P, Coleman R, Holen I
    BMC Cancer, 2015;15(0):55.
    Species: Human
    Sample Type: Cell Culture Supernates
  16. Pluripotency gene expression and growth control in cultures of peripheral blood monocytes during their conversion into programmable cells of monocytic origin (PCMO): evidence for a regulatory role of autocrine activin and TGF-beta.
    Authors: Ungefroren H, Hyder A, Hinz H, Groth S, Lange H, El-Sayed K, Ehnert S, Nussler A, Fandrich F, Gieseler F
    PLoS ONE, 2015;10(2):e0118097.
    Species: Human
    Sample Type: Cell Culture Supernates
  17. PMA induces SnoN proteolysis and CD61 expression through an autocrine mechanism.
    Authors: Li C, Peart N, Xuan Z, Lewis D, Xia Y, Jin J
    Cell Signal, 2014;26(7):1369-78.
    Species: Human
    Sample Type: Cell Culture Supernates
  18. Activin A induces growth arrest through a SMAD- dependent pathway in hepatic progenitor cells.
    Authors: Chen L, Zhang W, Liang H, Zhou Q, Ding Z, Yang H, Liu W, Wu Y, Man Q, Zhang B, Chen X
    Cell Commun Signal, 2014;12(0):18.
    Species: Rat
    Sample Type: Cell Culture Supernates
  19. Serum activin A and follistatin levels in gestational diabetes and the association of the Activin A-Follistatin system with anthropometric parameters in offspring.
    Authors: Naf S, Escote X, Ballesteros M, Yanez R, Simon-Muela I, Gil P, Albaiges G, Vendrell J, Megia A
    PLoS ONE, 2014;9(4):e92175.
    Species: Human
    Sample Type: Serum
  20. An essential role for insulin and IGF1 receptors in regulating sertoli cell proliferation, testis size, and FSH action in mice.
    Authors: Pitetti J, Calvel P, Zimmermann C, Conne B, Papaioannou M, Aubry F, Cederroth C, Urner F, Fumel B, Crausaz M, Docquier M, Herrera P, Pralong F, Germond M, Guillou F, Jegou B, Nef S
    Mol Endocrinol, 2013;27(5):814-27.
    Species: Mouse
    Sample Type: Serum
  21. Activin A is associated with impaired myocardial glucose metabolism and left ventricular remodeling in patients with uncomplicated type 2 diabetes.
    Authors: Chen W, Greulich S, van der Meer R, Rijzewijk L, Lamb H, de Roos A, Smit J, Romijn J, Ruige J, Lammertsma A, Lubberink M, Diamant M, Ouwens D
    Cardiovasc Diabetol, 2013;12(1):150.
    Species: Human
    Sample Type: Plasma
  22. Activin A skews macrophage polarization by promoting a proinflammatory phenotype and inhibiting the acquisition of anti-inflammatory macrophage markers.
    Authors: Sierra-Filardi E, Puig-Kroger A, Blanco FJ, Nieto C, Bragado R, Palomero MI, Bernabeu C, Vega MA, Corbi AL
    Blood, 2011;117(19):5092-101.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  23. Mechanisms inducing low bone density in duchenne muscular dystrophy in mice and humans.
    Authors: Rufo A, Del Fattore A, Capulli M, Carvello F, De Pasquale L, Ferrari S, Pierroz D, Morandi L, De Simone M, Rucci N, Bertini E, Bianchi ML, De Benedetti F, Teti A
    J. Bone Miner. Res., 2011;26(8):1891-903.
    Species: Human
    Sample Type: Serum
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Quantikine Immunoassay Control Set 586 for H/M/R Activin A

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Average Rating: 4.3 (Based on 4 reviews)

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