Human/Mouse/Rat Akt Pan Specific PE-conjugated Antibody

Catalog # Availability Size / Price Qty
IC2055P
Detection of Akt in Jurkat Human Cell Line by Flow Cytometry.
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Human/Mouse/Rat Akt Pan Specific PE-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Akt in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 281046
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Akt1
Ser2-Ala480
Accession # P31749
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection of Akt in Jurkat Human Cell Line by Flow Cytometry. View Larger

Detection of Akt in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human/Mouse/Rat Akt Pan Specific PE‑conjugated Monoclonal Antibody (Catalog # IC2055P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Akt

Akt (also known as PKB) is a 55-65 kDa member of the AGC kinase family of molecules. There are three Akt isoforms (Akt1, 2 and 3) that are virtually equal in length (approximately 480 amino acids), show 81-82% amino acid (aa) sequence identity, and are the produt of three distinct genes located on Ch14, Ch19 and Ch1, respectively. The "Akt" designation is based on an early mouse colony that was referred to as the "A" stock (or group), was inbred and generated a "k" strain, and which was ultimately recognized to contain a transforming, or "t" retrovirus that caused thymomas. All three Akts are Ser/Thr kinases that themselves are targets of the upstream PI3-kinase system. Although all three Akts are likely constitutively phosphorylated, 2-site Ser/Thr dual-phosphorylation by activated PI3-kinase appears to confer full enzyme activity. In addition to phosphorylation, the Akts are now known to undergo ubiquitination, SUMOlyation, tyrosine phosphorylation, Lysine acetylation and O-GlcNAcylation. This can result in a highly variable SDS-PAGE MW determination, and suggests the existence of a very complex regulatory system. There are more than 130 substrates for the Akts. The principal difference between the Akts lies in their expression pattern and intracellular localization. Akt1 is expressed ubiquitously, while Akt2 is concentrated in insulin-sensitive cell types (skeletal muscle; hepatocytes; adipocytes) and Akt3 is found in neurons and select cell types. Full-length human Akt1 is 98% aa sequence identical to mouse Akt1.

Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog
Alternate Names
RAC-ALPHA; AKT serine/threonine kinase 1; Akt; PKB; PKB-ALPHA; PRKBA; RAC

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