Detection of Akt in Jurkat Human Cell Line by Flow Cytometry.
Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human/Mouse/Rat Akt Pan Specific APC‑conjugated Monoclonal Antibody (Catalog # IC2055A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Akt (also known as PKB) is a 55-65 kDa member of the AGC kinase family of molecules. There are three Akt isoforms (Akt1, 2 and 3) that are virtually equal in length (approximately 480 amino acids), show 81-82% amino acid (aa) sequence identity, and are the produt of three distinct genes located on Ch14, Ch19 and Ch1, respectively. The "Akt" designation is based on an early mouse colony that was referred to as the "A" stock (or group), was inbred and generated a "k" strain, and which was ultimately recognized to contain a transforming, or "t" retrovirus that caused thymomas. All three Akts are Ser/Thr kinases that themselves are targets of the upstream PI3-kinase system. Although all three Akts are likely constitutively phosphorylated, 2-site Ser/Thr dual-phosphorylation by activated PI3-kinase appears to confer full enzyme activity. In addition to phosphorylation, the Akts are now known to undergo ubiquitination, SUMOlyation, tyrosine phosphorylation, Lysine acetylation and O-GlcNAcylation. This can result in a highly variable SDS-PAGE MW determination, and suggests the existence of a very complex regulatory system. There are more than 130 substrates for the Akts. The principal difference between the Akts lies in their expression pattern and intracellular localization. Akt1 is expressed ubiquitously, while Akt2 is concentrated in insulin-sensitive cell types (skeletal muscle; hepatocytes; adipocytes) and Akt3 is found in neurons and select cell types. Full-length human Akt1 is 98% aa sequence identical to mouse Akt1.
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