Human/Mouse/Rat Akt Pan Specific Antibody

Catalog # Availability Size / Price Qty
MAB2055
MAB2055-SP
Human/Mouse/Rat Akt Pan Specific Antibody in Western Blot
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Product Details
Citations (9)
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Human/Mouse/Rat Akt Pan Specific Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat Akt in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2B Clone # 281046
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Akt1
Ser2-Ala480
Accession # P31749
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Simple Western
2 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
1mmersion fixed paraffin-embedded sections of human breast cancer tissue and mouse spleen tissue
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human/Mouse Akt by Western Blot. View Larger

Detection of Human/Mouse Akt by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, MBA-MB-123 human breast cancer cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Western Blot Detection of Human Akt Pan Specific by Western Blot. View Larger

Detection of Human Akt Pan Specific by Western Blot. Western blot shows Recombinant Human Active Akt1 (Catalog # 1775-KS), recombinant human Akt2, and recombinant human Akt3 (5 ng/lane). PVDF membrane was probed with 0.2 µg/mL Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Immunocytochemistry Akt in MDA‑MB‑231 Human Cell Line. View Larger

Akt in MDA‑MB‑231 Human Cell Line. Akt was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Mouse Anti-Human/ Mouse/Rat Akt Monoclonal Antibody (Catalog # MAB2055) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunocytochemistry Akt (pan) in 293T Human Cell Line. View Larger

Akt (pan) in 293T Human Cell Line. Akt pan specific (panels A and C) and Akt1 (E17K Mutation) (panels B and D) were detected in immersion fixed 293T human embryonic kidney cell line transfected with wild type (panels A and B) or E17K mutated (panels C and D) Akt1 using Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055) and Mouse Anti-Human Akt1 (E17K Mutation) Monoclonal Antibody (Catalog # MAB6815). Both antibodies were used at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to plasma membranes and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry View Larger

Akt Pan Specific in Human Breast Cancer Tissue. Akt was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunohistochemistry View Larger

Akt Pan Specific in Mouse Spleen Tissue. Akt was detected in immersion fixed paraffin-embedded sections of mouse spleen tissue using Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Intracellular Staining by Flow Cytometry Detection of Akt in MCF‑7 Human Cell Line by Flow Cytometry. View Larger

Detection of Akt in MCF‑7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human/Mouse/Rat Akt Monoclonal Antibody (Catalog # MAB2055, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Simple Western Detection of Human Akt by Simple Western<sup>TM</sup>. View Larger

Detection of Human Akt by Simple WesternTM. Simple Western lane view shows lysates of MCF‑7 human breast cancer cell line, loaded at 0.2 mg/mL. A specific band was detected for Akt at approximately 62 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat Akt Pan Specific Monoclonal Antibody (Catalog # MAB2055). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Akt

Akt, also known as protein kinase B (PKB), is a central kinase in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. Three highly homologous members define the Akt family: Akt1 (PKB alpha ), Akt2 (PKB beta ), and Akt3 (PKB gamma ). All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.

Long Name
v-Akt Murine Thymoma Viral Oncogene Homolog
Alternate Names
RAC-ALPHA; AKT serine/threonine kinase 1; Akt; PKB; PKB-ALPHA; PRKBA; RAC

Product Datasheets

Citations for Human/Mouse/Rat Akt Pan Specific Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Jejunal Insulin Signalling Is Increased in Morbidly Obese Subjects with High Insulin Resistance and Is Regulated by Insulin and Leptin
    Authors: C Gutierrez-, A Ho-Plagaro, C Santiago-F, S Garcia-Ser, F Rodríguez-, S Valdes, L Garrido-Sa, C Rodríguez-, C López-Góme, FJ Moreno-Rui, G Alcain-Mar, A Gautier-St, G Mithieux, E Garcia-Fue
    J Clin Med, 2020;9(1):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. C16 Peptide Promotes Vascular Growth and Reduces Inflammation in a Neuromyelitis Optica Model
    Authors: H Chen, X Fu, J Jiang, S Han
    Front Pharmacol, 2019;10(0):1373.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  3. Endocrine responses and acute mTOR pathway phosphorylation to resistance exercise with leucine and whey
    Authors: MT Lane, TJ Herda, AC Fry, MA Cooper, MJ Andre, PM Gallagher
    Biol Sport, 2017;34(2):197-203.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Infared Microscopy
  4. Subthalamic Nucleus Deep Brain Stimulation Employs trkB Signaling for Neuroprotection and Functional Restoration
    Authors: DL Fischer, CJ Kemp, A Cole-Strau, NK Polinski, KL Paumier, JW Lipton, K Steece-Col, TJ Collier, DJ Buhlinger, CE Sortwell
    J. Neurosci., 2017;37(28):6786-6796.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  5. Adipocyte SIRT1 controls systemic insulin sensitivity by modulating macrophages in adipose tissue
    Authors: X Hui, M Zhang, P Gu, K Li, Y Gao, D Wu, Y Wang, A Xu
    EMBO Rep, 2017;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  6. Lack of CD2AP disrupts Glut4 trafficking and attenuates glucose uptake in podocytes.
    Authors: Tolvanen T, Dash S, Polianskyte-Prause Z, Dumont V, Lehtonen S
    J Cell Sci, 2015;128(24):4588-600.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Single amino Acid substitutions in the chemotactic sequence of urokinase receptor modulate cell migration and invasion.
    Authors: Bifulco, Katia, Longanesi-Cattani, Immacola, Franco, Paola, Pavone, Vincenzo, Mugione, Pietro, Di Carluccio, Gioconda, Masucci, Maria Te, Arra, Claudio, Pirozzi, Giuseppe, Stoppelli, Maria Pa, Carriero, Maria Vi
    PLoS ONE, 2012;7(9):e44806.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation.
    Authors: Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C
    J. Mol. Cell. Cardiol., 2010;48(6):1225-35.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt.
    Authors: Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S
    Apoptosis, 2010;15(4):463-73.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot

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