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Phospho-MBP (T98) Antibody

Human/Mouse/Rat/Bovine
Phospho-MBP (T98) Antibody

Catalog #: MAB4228
1 Citation Datasheet / COA / SDS

Discontinued Product

MAB4228 has been discontinued.
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Detection of Bovine Phospho-MBP (T98) by Western Blot.
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Citations (1)
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Human/Mouse/Rat/Bovine
Phospho-MBP (T98) Antibody Summary

Species Reactivity
Human, Mouse, Rat, Bovine
Specificity
Detects in vitro-phosphorylated MBP (T98) in Western blots. Based on sequence homology of the immunogen, this antibody should react with phosphorylated MBP from most common vertebrate species, including human, mouse, rat, and bovine.
Source
Monoclonal Mouse IgG2B Clone # 399126
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing human MBP T98 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Bovine Phospho-MBP (T98) antibody by Western Blot. View Larger

Detection of Bovine Phospho-MBP (T98) by Western Blot. Western blot shows lysates of bovine brain tissue treated with the indicated concentration of Recombinant Human p38a (Active) (Catalog # 1806-P3) for 30 minutes. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat/Bovine Phospho-MBP (T98) Monoclonal Antibody (Catalog # MAB4228) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Phospho-MBP (T98) was detected at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MBP

Myelin basic protein (MBP) is the most abundant protein component of the myelin membrane in the central nervous system. MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98.

Long Name
Myelin Basic Protein
Entrez Gene IDs
4155 (Human); 17196 (Mouse); 24547 (Rat)
Alternate Names
Hmbpr; MBP; MGC99675; mld; Myelin A1 protein; myelin basic protein; Myelin membrane encephalitogenic protein; shi

Product Datasheets

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Citation for Human/Mouse/Rat/Bovine
Phospho-MBP (T98) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Curcumin protects pre-oligodendrocytes from activated microglia in vitro and in vivo.
    Authors: He LF, Chen HJ, Qian LH, Chen GY, Buzby JS
    Brain Res., 2010-04-24;1339(0):60-9.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC

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Phospho-MBP (T98) Antibody

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