Detects in vitro-phosphorylated MBP (T98) in Western blots. Based on sequence homology of the immunogen, this antibody should react with phosphorylated MBP from most common vertebrate species, including human, mouse, rat, and bovine.
Monoclonal Mouse IgG2B Clone # 399126
Protein A or G purified from hybridoma culture supernatant
Phosphopeptide containing human MBP T98 site
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Bovine Phospho-MBP (T98) by Western Blot.
Western blot shows lysates of bovine brain tissue treated with the indicated concentration of Recombinant Human p38 alpha (Active) (Catalog # 1806-P3) for 30 minutes. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat/Bovine Phospho-MBP (T98) Monoclonal Antibody (Catalog # MAB4228) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for Phospho-MBP (T98) was detected at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Myelin basic protein (MBP) is the most abundant protein component of the myelin membrane in the central nervous system. MBP has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. In vitro, MBP is suitable as a substrate for numerous protein kinases, including the ERK and p38 MAP kinases that phosphorylate MBP at T98.
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