Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Alexa Fluor® 647-conjugated Antibody

    
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  • Species Reactivity
    Human, Mouse, Rat, Chicken
  • Specificity
    Detects human, mouse, rat and chicken Oligodendrocyte Marker O1.
  • Source
    Monoclonal Mouse IgM Clone # O1
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    Bovine brain corpus callosum white matter
  • Formulation
    Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25-1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem Cells by Flow Cytometry. Rat differentiated cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1327R, filled histogram) or mouse IgM isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Oligodendrocyte Marker O1
Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O1 recognizes a glycolipid antigen that is expressed on the surface of late oligodendrocyte progenitors. It has been commonly used in conjunction with Oligodendrocyte Marker O4 antibody to define immature oligodendrocyte (1-6). Progenitors that are O4 antigen-positive and O1 antigen-negative have been shown to differentiate into O1 antigen-positive oligodendrocytes in vitro (7).
  • References:
    1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
    2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
    3. Sontheimer, H. et al. (1989) Neuron 2:1135.
    4. Hardy, R.J. and V.L. Friedrich Jr. (1996) Development 122:2059.
    5. Reynolds, R. and R. Hardy (1997) J. Neurosci. Res. 47:455.
    6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
    7. Cai, Z. et al. (2001) Brain Res. 898:126.
  • Alternate Names:
    Oligodendrocyte Marker O1
Related Research Areas

FAQs

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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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