Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Alexa Fluor® 647-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1327R-100UG
Detection  of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem  Cells by Flow Cytometry.
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Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Detects human, mouse, rat and chicken Oligodendrocyte Marker O1.
Source
Monoclonal Mouse IgM Clone # O1
Purification
IgM-specific Affinity-purified from hybridoma culture supernatant
Immunogen
Bovine brain corpus callosum white matter
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection  of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem  Cells by Flow Cytometry. View Larger

Detection of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem Cells by Flow Cytometry. Rat differentiated cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB1327R, filled histogram) or mouse IgM isotype control antibody (open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Oligodendrocyte Marker O1

Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O1 recognizes a glycolipid antigen that is expressed on the surface of late oligodendrocyte progenitors. It has been commonly used in conjunction with Oligodendrocyte Marker O4 antibody to define immature oligodendrocyte (1-6). Progenitors that are O4 antigen-positive and O1 antigen-negative have been shown to differentiate into O1 antigen-positive oligodendrocytes in vitro (7).

References
  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Sontheimer, H. et al. (1989) Neuron 2:1135.
  4. Hardy, R.J. and V.L. Friedrich Jr. (1996) Development 122:2059.
  5. Reynolds, R. and R. Hardy (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Cai, Z. et al. (2001) Brain Res. 898:126.
Alternate Names
Oligodendrocyte Marker O1

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