Oligodendrocyte Marker O1 Antibody (O1)

R&D Systems | Catalog # MAB1327

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken

Cited:

Human, Mouse, Rat, Primate - Papio anubis (Olive Baboon)

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Bioassay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # O1
View all Oligodendrocyte Marker O1 Primary Antibodies »

Product Specifications

Immunogen

Bovine brain corpus callosum white matter

Specificity

Detects human, mouse, rat and chicken Oligodendrocyte Marker O1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for Oligodendrocyte Marker O1 Antibody (O1)

Detection of Oligodendrocyte Marker O1 antibody in Rat Differentiated Cortical Stem Cells antibody by Flow Cytometry.

Detection of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem Cells by Flow Cytometry.

Rat differentiated cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Monoclonal Antibody (Catalog # MAB1327, filled histogram) or mouse IgM isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.
Oligodendrocyte Marker O1 antibody in Rat Cortical Stem Cells by Immunocytochemistry (ICC).

Oligodendrocyte Marker O1 in Rat Cortical Stem Cells.

Oligodendrocyte Marker O1 was detected in immersion fixed 7 day differentiated rat cortical stem cells using Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Monoclonal Antibody (Catalog # MAB1327) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). Specific staining was localized to oligodendrocytes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Oligodendrocyte Marker O1 Antibody (O1)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Rat differentiated cortical stem cells

Immunocytochemistry

1-25 µg/mL
Sample: Immersion fixed 7 day differentiated rat cortical stem cells

Reviewed Applications

Read 1 review rated 4 using MAB1327 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

IgM-specific Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Oligodendrocyte Marker O1

Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O1 recognizes a glycolipid antigen that is expressed on the surface of late oligodendrocyte progenitors. It has been commonly used in conjunction with Oligodendrocyte Marker O4 antibody to define immature oligodendrocyte (1-6). Progenitors that are O4 antigen-positive and O1 antigen-negative have been shown to differentiate into O1 antigen-positive oligodendrocytes in vitro (7).

References

  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Sontheimer, H. et al. (1989) Neuron 2:1135.
  4. Hardy, R.J. and V.L. Friedrich Jr. (1996) Development 122:2059.
  5. Reynolds, R. and R. Hardy (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Cai, Z. et al. (2001) Brain Res. 898:126.

Alternate Names

Marker

Additional Oligodendrocyte Marker O1 Products

Product Documents for Oligodendrocyte Marker O1 Antibody (O1)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Oligodendrocyte Marker O1 Antibody (O1)

For research use only

Related Research Areas

Glial Lineage Markers

Citations for Oligodendrocyte Marker O1 Antibody (O1)

Customer Reviews for Oligodendrocyte Marker O1 Antibody (O1) (1)

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  • Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: IPS2 induced pluripotent stem cells
    Species: Mouse
    Verified Customer | Posted 01/25/2018
    Oligodendrocyte Marker O1 Antibody (O1) MAB1327

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Protocols

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