Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Antibody

(4 citations)   
  • Species Reactivity
    Human, Mouse, Rat, Chicken
  • Specificity
    Detects human, mouse, rat and chicken Oligodendrocyte Marker O1.
  • Source
    Monoclonal Mouse IgM Clone # O1
  • Purification
    IgM-specific Affinity-purified from hybridoma culture supernatant
  • Immunogen
    Bovine brain corpus callosum white matter
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    1-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Oligodendrocyte Marker O1 in Rat Differentiated Cortical Stem Cells by Flow Cytometry. Rat differentiated cortical stem cells were stained with Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Monoclonal Antibody (Catalog # MAB1327, filled histogram) or mouse IgM isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0117). View our protocol for Staining Membrane-associated Proteins.
Immunocytochemistry
Oligodendrocyte Marker O1 in Rat Cortical Stem Cells. Oligodendrocyte Marker O1 was detected in immersion fixed 7 day differentiated rat cortical stem cells using Mouse Anti-Human/Mouse/Rat/Chicken Oligodendrocyte Marker O1 Monoclonal Antibody (Catalog # MAB1327) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgM Secondary Antibody (red; Catalog # NL019) and counterstained with DAPI (blue). Specific staining was localized to oligodendrocytes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Oligodendrocyte Marker O1
Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O1 recognizes a glycolipid antigen that is expressed on the surface of late oligodendrocyte progenitors. It has been commonly used in conjunction with Oligodendrocyte Marker O4 antibody to define immature oligodendrocyte (1-6). Progenitors that are O4 antigen-positive and O1 antigen-negative have been shown to differentiate into O1 antigen-positive oligodendrocytes in vitro (7).
  • References:
    1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
    2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
    3. Sontheimer, H. et al. (1989) Neuron 2:1135.
    4. Hardy, R.J. and V.L. Friedrich Jr. (1996) Development 122:2059.
    5. Reynolds, R. and R. Hardy (1997) J. Neurosci. Res. 47:455.
    6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
    7. Cai, Z. et al. (2001) Brain Res. 898:126.
  • Alternate Names:
    Oligodendrocyte Marker O1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Applications
Sample Type
  1. Characterization of oligodendroglial populations in mouse demyelinating disease using flow cytometry: clues for MS pathogenesis.
    Authors: Robinson A, Rodgers J, Goings G, Miller S
    PLoS ONE, 2015;9(9):e107649.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Curcumin protects pre-oligodendrocytes from activated microglia in vitro and in vivo.
    Authors: He LF, Chen HJ, Qian LH, Chen GY, Buzby JS
    Brain Res., 2010;1339(0):60-9.
    Species: Rat
    Sample Type: Whole Cells
    Application: ICC
  3. Efficient induction of oligodendrocytes from human embryonic stem cells.
    Authors: Kang SM, Cho MS, Seo H, Yoon CJ, Oh SK, Choi YM, Kim DW
    Stem Cells, 2006;25(2):419-24.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. Excitatory amino acid induced oligodendrocyte cell death in vitro: receptor-dependent and -independent mechanisms.
    Authors: Rosin C, Bates TE, Skaper SD
    J. Neurochem., 2004;90(5):1173-85.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
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Goat Anti-Mouse IgM PerCP-conjugated Antibody

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