Oligodendrocyte Marker O1 Alexa Fluor® 405-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1327V-100UG
R&D Systems Antibodies
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Oligodendrocyte Marker O1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat, Chicken
Specificity
Detects human, mouse, rat and chicken Oligodendrocyte Marker O1.
Source
Monoclonal Mouse IgM Clone # O1
Immunogen
Bovine brain corpus callosum white matter
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
Rat differentiated cortical stem cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Oligodendrocyte Marker O1

Oligodendrocytes are myelinating cells in the central nervous system (CNS) that form the myelin sheath of axons to support rapid nerve conduction. Oligodendrocyte Marker O1 recognizes a glycolipid antigen that is expressed on the surface of late oligodendrocyte progenitors. It has been commonly used in conjunction with Oligodendrocyte Marker O4 antibody to define immature oligodendrocyte (1-6). Progenitors that are O4 antigen-positive and O1 antigen-negative have been shown to differentiate into O1 antigen-positive oligodendrocytes in vitro (7).

References
  1. Schachner, M. et al. (1981) Dev. Biol. 83:328.
  2. Bansal, R. et al. (1989) J. Neurosci. Res. 24:548.
  3. Sontheimer, H. et al. (1989) Neuron 2:1135.
  4. Hardy, R.J. and V.L. Friedrich Jr. (1996) Development 122:2059.
  5. Reynolds, R. and R. Hardy (1997) J. Neurosci. Res. 47:455.
  6. Ono, K. et al. (1997) J. Neurosci. Res. 48:212.
  7. Cai, Z. et al. (2001) Brain Res. 898:126.
Alternate Names
Marker; Oligodendrocyte Marker O1

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