Human/Mouse/Rat EMP/MAEA Antibody

Name: Human/Mouse/Rat EMP/MAEA Antibody
Brand: R&D Systems
SKU: MAB7288

R&D Systems | Catalog # MAB7288

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Product Documents

Discontinued Product

MAB7288 has been discontinued. An alternative/replacement product is available: AF7288. View all EMP/MAEA products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 730340

View all EMP/MAEA Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human EMP/MAEA
Met12-Ser66
Accession # Q7L5Y9

Specificity

Detects human EMP/MAEA in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Mouse/Rat EMP/MAEA Antibody

Detection of Human, Mouse, and Rat EMP/MAEA by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, NR8383 rat alveolar macrophage cell line, and Y3‑Ag rat myeloid cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat EMP/MAEA Monoclonal Antibody (Catalog # MAB7288) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for EMP/MAEA at approximately 36-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EMP/MAEA in Jurkat Human Cell Line.

EMP/MAEA was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Mouse Anti-Human/Mouse/Rat EMP/MAEA Monoclonal Antibody (Catalog # MAB7288) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. This application has not been tested in mouse or rat samples.

Applications for Human/Mouse/Rat EMP/MAEA Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, C2C12 mouse myoblast cell line, NR8383 rat alveolar macrophage cell line, and Y3‑Ag rat myeloid cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: EMP/MAEA

EMP (erythroblast macrophage protein), also called MAEA (macrophage-erythroblast attacher), is a 36-44 kDa membrane-associated protein in macrophages and erythroblasts within erythroblastic islands. EMP is essential to the process of nuclear extrusion in the transition of erythroblasts to reticulocytes. Within the region used as an immunogen, human EMP shares 98% amino acid (aa) sequence identity with mouse and rat EMP. Four potential isoforms of the canonical 396 aa form encode 360, 355, 328 and 245 aa, with insertions and deletions occurring after aa 150.

Long Name

Erythroblast Macrophage Protein/Macrophage Erythroblast Attacher

Alternate Names

EMLP, HLC-10, MAEA, PIG5

Entrez Gene IDs

10296 (Human); 59003 (Mouse)

Gene Symbol

MAEA

UniProt

Q7L5Y9 (Human)

Additional EMP/MAEA Products

Product Documents for Human/Mouse/Rat EMP/MAEA Antibody

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat EMP/MAEA Antibody

For research use only

Related Research Areas

Red Blood Cells (RBCs)

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