Human/Mouse/Rat EMP/MAEA Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat EMP/MAEA by Western Blot.
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Product Details
Citations (2)
Supplemental Products

Human/Mouse/Rat EMP/MAEA Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse and rat EMP/MAEA in Western blots. Detects recombinant human EMP/MAEA in direct ELISAs.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human EMP/MAEA
Accession # Q7L5Y9
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat EMP/MAEA antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat EMP/MAEA by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMP/MAEA at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry EMP/MAEA antibody in Jurkat Human Cell Line by Immunocytochemistry (ICC). View Larger

EMP/MAEA in Jurkat Human Cell Line. EMP/MAEA was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMP/MAEA

EMP (erythroblast macrophage protein), also called MAEA (macrophage-erythroblast attacher), is a 36-45 kDa membrane-associated protein in macrophages and erythroblasts within erythroblastic islands. EMP is essential to the process of nuclear extrusion in the transition of erythroblasts to reticulocytes. Within the region used as an immunogen, human EMP shares 98% amino acid (aa) sequence identity with mouse and rat EMP. Four potential isoforms of the canonical 396 aa form encode 360, 355, 328 and 245 aa, with insertions and deletions occurring after aa 150.

Long Name
Erythroblast Macrophage Protein/Macrophage Erythroblast Attacher
Entrez Gene IDs
10296 (Human); 59003 (Mouse)
Alternate Names
EMLP; EMP; EMPCell proliferation-inducing gene 5 protein; Erythroblast macrophage protein; HLC-10; Human lung cancer oncogene 10 protein; lung cancer-related protein 10; macrophage erythroblast attacher; MAEA; PIG5; proliferation-inducing gene 5

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Citations for Human/Mouse/Rat EMP/MAEA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin
    Authors: SHA Soliman, AE Stark, ML Gardner, SW Harshman, CC Breece, F Amari, A Orlacchio, M Chen, A Tessari, JA Martin, R Visone, MA Freitas, KMD La Perle, D Palmieri, V Coppola
    Sci Rep, 2020;10(1):7138.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation
    Authors: MER Maitland, G Onea, CA Chiasson, X Wang, J Ma, SE Moor, KR Barber, GA Lajoie, GS Shaw, C Schild-Pou
    Sci Rep, 2019;9(1):9864.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot


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