Human/Mouse/Rat EMP/MAEA Antibody

Catalog #: AF7288
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7288
AF7288-SP

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Detection of Human, Mouse, and Rat EMP/MAEA by Western Blot.

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Product Details

Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat EMP/MAEA Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse and rat EMP/MAEA in Western blots. Detects recombinant human EMP/MAEA in direct ELISAs.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human EMP/MAEA
Met12-Ser66
Accession # Q7L5Y9

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human, Mouse, and Rat EMP/MAEA by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMP/MAEA at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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EMP/MAEA in Jurkat Human Cell Line. EMP/MAEA was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMP/MAEA

EMP (erythroblast macrophage protein), also called MAEA (macrophage-erythroblast attacher), is a 36-45 kDa membrane-associated protein in macrophages and erythroblasts within erythroblastic islands. EMP is essential to the process of nuclear extrusion in the transition of erythroblasts to reticulocytes. Within the region used as an immunogen, human EMP shares 98% amino acid (aa) sequence identity with mouse and rat EMP. Four potential isoforms of the canonical 396 aa form encode 360, 355, 328 and 245 aa, with insertions and deletions occurring after aa 150.

Long Name

Erythroblast Macrophage Protein/Macrophage Erythroblast Attacher

Entrez Gene IDs

10296 (Human); 59003 (Mouse)

Alternate Names

EMLP; EMP; EMPCell proliferation-inducing gene 5 protein; Erythroblast macrophage protein; HLC-10; Human lung cancer oncogene 10 protein; lung cancer-related protein 10; macrophage erythroblast attacher; MAEA; PIG5; proliferation-inducing gene 5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Red Blood Cells (RBCs)

Citations for Human/Mouse/Rat EMP/MAEA Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Tagging enhances histochemical and biochemical detection of Ran Binding Protein 9 in vivo and reveals its interaction with Nucleolin
Authors: SHA Soliman, AE Stark, ML Gardner, SW Harshman, CC Breece, F Amari, A Orlacchio, M Chen, A Tessari, JA Martin, R Visone, MA Freitas, KMD La Perle, D Palmieri, V Coppola
Sci Rep, 2020;10(1):7138.
Species: Mouse
Sample Types: Cell Lysates
Applications: Immunoprecipitation
The mammalian CTLH complex is an E3 ubiquitin ligase that targets its subunit muskelin for degradation
Authors: MER Maitland, G Onea, CA Chiasson, X Wang, J Ma, SE Moor, KR Barber, GA Lajoie, GS Shaw, C Schild-Pou
Sci Rep, 2019;9(1):9864.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot