Human/Mouse/Rat EMP/MAEA Antibody

Catalog # Availability Size / Price Qty
AF7288
AF7288-SP
Detection of Human, Mouse, and Rat EMP/MAEA by Western Blot.
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Human/Mouse/Rat EMP/MAEA Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human, mouse and rat EMP/MAEA in Western blots. Detects recombinant human EMP/MAEA in direct ELISAs.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human EMP/MAEA
Met12-Ser66
Accession # Q7L5Y9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat EMP/MAEA by Western Blot. View Larger

Detection of Human, Mouse, and Rat EMP/MAEA by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMP/MAEA at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry EMP/MAEA in Jurkat Human Cell Line. View Larger

EMP/MAEA in Jurkat Human Cell Line. EMP/MAEA was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EMP/MAEA

EMP (erythroblast macrophage protein), also called MAEA (macrophage-erythroblast attacher), is a 36-45 kDa membrane-associated protein in macrophages and erythroblasts within erythroblastic islands. EMP is essential to the process of nuclear extrusion in the transition of erythroblasts to reticulocytes. Within the region used as an immunogen, human EMP shares 98% amino acid (aa) sequence identity with mouse and rat EMP. Four potential isoforms of the canonical 396 aa form encode 360, 355, 328 and 245 aa, with insertions and deletions occurring after aa 150.

Long Name
Erythroblast Macrophage Protein/Macrophage Erythroblast Attacher
Entrez Gene IDs
10296 (Human); 59003 (Mouse)
Alternate Names
EMLP; EMP; EMPCell proliferation-inducing gene 5 protein; Erythroblast macrophage protein; HLC-10; Human lung cancer oncogene 10 protein; lung cancer-related protein 10; macrophage erythroblast attacher; MAEA; PIG5; proliferation-inducing gene 5

Product Datasheets

FAQs

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