Detects endogenous human, mouse, and rat FKBP13 at 13 kDa in Western blots. In Western blots, this antibody does not cross-react with recombinant human FKBP12, FKBP12.6, FKBP25, FKBP38, FKBP51, or FKBP52.
Monoclonal Rat IgG2B Clone # 422617
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human FKBP13 Ala22-Leu142 Accession # P26885
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human and Rat FKBP13 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, PC‑12 rat adrenal pheochromocytoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP13 Monoclonal Antibody (Catalog # MAB4356) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP13 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
FK506 binding protein, 13 kDa molecular weight (FKBP13), also called FKBP2, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressant FK506 is a potent FKBP13 inhibitor. FKBP13 is strongly induced by calcium ionophores and agents that cause endoplasmic reticulum stress, such as tunicamycin. FKBP13 is membrane-associated and tightly bound to the cytoskeletal protein Band 4.1 in erythrocytes, suggesting a role in maintaining structural proteins.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.