Human/Mouse/Rat FKBP13 Antibody

  
  • Species Reactivity
    Human, Mouse, Rat
  • Specificity
    Detects endogenous human, mouse, and rat FKBP13 at 13 kDa in Western blots. In Western blots, this antibody does not cross-react with recombinant human FKBP12, FKBP12.6, FKBP25, FKBP38, FKBP51, or FKBP52.
  • Source
    Monoclonal Rat IgG2B Clone # 422617
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human FKBP13
    Ala22-Leu142
    Accession # P26885
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human and Rat FKBP13 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, PC‑12 rat adrenal pheochromocytoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP13 Monoclonal Antibody (Catalog # MAB4356) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP13 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FKBP13

FK506 binding protein, 13 kDa molecular weight (FKBP13), also called FKBP2, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressant FK506 is a potent FKBP13 inhibitor. FKBP13 is strongly induced by calcium ionophores and agents that cause endoplasmic reticulum stress, such as tunicamycin. FKBP13 is membrane-associated and tightly bound to the cytoskeletal protein Band 4.1 in erythrocytes, suggesting a role in maintaining structural proteins.

  • Long Name:
    13 kDa FK506 Binding Protein
  • Entrez Gene IDs:
    2286 (Human); 14227 (Mouse); 25639 (Rat)
  • Alternate Names:
    EC 5.2.1.8,13 kDa FK506-binding protein; FK506 binding protein 2, 13kDa; FK506-binding protein 2 (13kD); FK506-binding protein 2; FKBP13; FKBP13,13 kDa FKBP; FKBP-13proline isomerase; FKBP2; FKBP-2; Immunophilin FKBP13; peptidyl-prolyl cis-trans isomerase FKBP2; PPIase FKBP2; PPIase; rapamycin-binding protein; rotamase
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Isotype Control

Ctrl MAB0061 27  
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Normal Rat IgG Control

Ctrl 6-001-A 9
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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2B Isotype Control

Ctrl MAB0061R
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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