Human/Mouse/Rat FKBP13 Antibody

Name: Human/Mouse/Rat FKBP13 Antibody
Brand: R&D Systems
SKU: MAB4356-SP
Rating: 3.5 (2 reviews)

Catalog #: MAB4356 2 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4356
MAB4356-SP

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Detection of Human and Rat FKBP13 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat FKBP13 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse, and rat FKBP13 at 13 kDa in Western blots. In Western blots, this antibody does not cross-react with recombinant human FKBP12, FKBP12.6, FKBP25, FKBP38, FKBP51, or FKBP52.

Source

Monoclonal Rat IgG_2B Clone # 422617

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human FKBP13
Ala22-Leu142
Accession # P26885

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human and Rat FKBP13 by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP13 Monoclonal Antibody (Catalog # MAB4356) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP13 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FKBP13

FK506 binding protein, 13 kDa molecular weight (FKBP13), also called FKBP2, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressant FK506 is a potent FKBP13 inhibitor. FKBP13 is strongly induced by calcium ionophores and agents that cause endoplasmic reticulum stress, such as tunicamycin. FKBP13 is membrane-associated and tightly bound to the cytoskeletal protein Band 4.1 in erythrocytes, suggesting a role in maintaining structural proteins.

Long Name

13 kDa FK506 Binding Protein

Entrez Gene IDs

2286 (Human); 14227 (Mouse); 25639 (Rat)

Alternate Names

EC 5.2.1.8,13 kDa FK506-binding protein; FK506 binding protein 2, 13kDa; FK506-binding protein 2 (13kD); FK506-binding protein 2; FKBP13; FKBP13,13 kDa FKBP; FKBP-13proline isomerase; FKBP2; FKBP-2; Immunophilin FKBP13; peptidyl-prolyl cis-trans isomerase FKBP2; PPIase FKBP2; PPIase; rapamycin-binding protein; rotamase