Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 422617
View all FKBP13 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human FKBP13
Ala22-Leu142
Accession # P26885

Specificity

Detects endogenous human, mouse, and rat FKBP13 at 13 kDa in Western blots. In Western blots, this antibody does not cross-react with recombinant human FKBP12, FKBP12.6, FKBP25, FKBP38, FKBP51, or FKBP52.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat FKBP13 Antibody

Detection of Human and Rat FKBP13 antibody by Western Blot.

Detection of Human and Rat FKBP13 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP13 Monoclonal Antibody (Catalog # MAB4356) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP13 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat FKBP13 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line

Reviewed Applications

Read 2 reviews rated 3.5 using MAB4356 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FKBP13

FK506 binding protein, 13 kDa molecular weight (FKBP13), also called FKBP2, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressant FK506 is a potent FKBP13 inhibitor. FKBP13 is strongly induced by calcium ionophores and agents that cause endoplasmic reticulum stress, such as tunicamycin. FKBP13 is membrane-associated and tightly bound to the cytoskeletal protein Band 4.1 in erythrocytes, suggesting a role in maintaining structural proteins.

Long Name

13 kDa FK506 Binding Protein

Alternate Names

FKBP2

Entrez Gene IDs

2286 (Human); 14227 (Mouse); 25639 (Rat)

Gene Symbol

FKBP2

UniProt

P26885

Additional FKBP13 Products

Product Documents for Human/Mouse/Rat FKBP13 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat FKBP13 Antibody

For research use only

Related Research Areas

Phosphatases and Regulators

Citations for Human/Mouse/Rat FKBP13 Antibody

Customer Reviews for Human/Mouse/Rat FKBP13 Antibody (2)

3.5 out of 5
2 Customer Ratings
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  • Human/Mouse/Rat FKBP13 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 03/11/2019
    Human/Mouse/Rat FKBP13 Antibody MAB4356
  • Human/Mouse/Rat FKBP13 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/21/2019

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