Human/Mouse/Rat FLIP Alexa Fluor® 532-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: FLIP

FLIP (Flice-Like Inhibitory Protein), also known as CFLAR (CASP8 and FADD-Like Apoptosis Regulator), I-FLICE, CASPER, and FLAME-1, is an apoptosis inhibitory protein with architecture similar to that of Caspases-8 and -10. Both of the major FLIP isoforms, the 55 kDa Long (L) and the 27 kDa Short (S), contain two death effector domains (DED).  FLIP(L) has a C-terminal Caspase-like protease domain which lacks both a catalytic active site and residues that form a substrate-binding pocket. FLIP(S) and FLIP(L) interact with the adaptor molecule FADD, and potently inhibit apoptosis initiated by Fas Ligand/TNF signaling pathways.  Over amino acids 1-200, human FLIP shares 75% sequence identity with mouse and rat FLIP.

Product Datasheets

Related Research Areas

• Adaptor Proteins
• Adaptor Proteins in the Akt Pathway
• Apoptosis Adaptor Proteins
• Caspases and Regulators
• Cysteine Proteases and Regulators
• TNF Superfamily
• TNF Superfamily Regulators