Human/Mouse/Rat FLIP Antibody

Catalog # Availability Size / Price Qty
MAB8430
MAB8430-SP
Detection of Human, Mouse, and Rat FLIP by Western Blot.
2 Images
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Human/Mouse/Rat FLIP Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects human FLIP in direct ELISAs and human, mouse, and rat FLIP in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 896537
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human FLIP
Met1-Asn200
Accession # O15519
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human, Mouse, and Rat FLIP antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat FLIP by Western Blot. Western blot shows lysates of PANC-1 human pancreatic carcinoma cell line, L-929 mouse fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat FLIP Monoclonal Antibody (Catalog # MAB8430) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for FLIP at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry FLIP antibody in Human Pancreatic Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

FLIP in Human Pancreatic Cancer Tissue. FLIP was detected in immersion fixed paraffin-embedded sections of human pancreatic cancer tissue using Mouse Anti-Human/Mouse/Rat FLIP Monoclonal Antibody (Catalog # MAB8430) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of cells in islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FLIP

FLIP (Flice-Like Inhibitory Protein), also known as CFLAR (CASP8 and FADD-Like Apoptosis Regulator), I-FLICE, CASPER, and FLAME-1, is an apoptosis inhibitory protein with architecture similar to that of Caspases-8 and -10. Both of the major FLIP isoforms, the 55 kDa Long (L) and the 27 kDa Short (S), contain two death effector domains (DED).  FLIP(L) has a C-terminal Caspase-like protease domain which lacks both a catalytic active site and residues that form a substrate-binding pocket. FLIP(S) and FLIP(L) interact with the adaptor molecule FADD, and potently inhibit apoptosis initiated by Fas Ligand/TNF signaling pathways.  Over amino acids 1-200, human FLIP shares 75% sequence identity with mouse and rat FLIP.

Long Name
FLICE Inhibitory Proteins
Entrez Gene IDs
8837 (Human); 12633 (Mouse)
Alternate Names
CASHFADD-like antiapoptotic molecule 1; CASP8 and FADD-like apoptosis regulator; CASP8AP1; Caspase homolog; Caspase-eight-related protein; Caspase-like apoptosis regulatory protein; caspase-related inducer of apoptosis; CASPER; CasperFLAME1; Cellular FLICE-like inhibitory protein; CFLAR; c-FLIPR; c-FLIPS; CLARPMACH-related inducer of toxicity; FADD-like apoptotic molecule; FLAME; FLAME-1; FLIP; I-FLICE; I-FLICEMRITc-FLIPFLIPc-FLIPL; Inhibitor of FLICE; usurpin beta; Usurpin

Product Datasheets

Citation for Human/Mouse/Rat FLIP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Induction of immunosuppressive functions and NF-?B by FLIP in monocytes
    Authors: A Fiore, S Ugel, F De Sanctis, S Sandri, G Fracasso, R Trovato, S Sartoris, S Solito, S Mandruzzat, F Vascotto, KL Hippen, G Mondanelli, U Grohmann, G Piro, C Carbone, D Melisi, RT Lawlor, A Scarpa, A Lamolinara, M Iezzi, M Fassan, S Bicciato, BR Blazar, U Sahin, PJ Murray, V Bronte
    Nat Commun, 2018;9(1):5193.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human/Mouse/Rat FLIP Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat FLIP Antibody and earn rewards!

Have you used Human/Mouse/Rat FLIP Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review