Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

R&D Systems | Catalog # PPS013

R&D Systems
Discontinued Product
PPS013 has been discontinued. View all GRIN2B/NMDAR2B products.

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all GRIN2B/NMDAR2B Primary Antibodies »

Product Specifications

Immunogen

Fusion protein from the C-terminus of the NR2B-subunit of rat NMDA Receptor

Specificity

Human, mouse, rat ~180 kDa NR2B-subunit of the NMDA Receptor

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

Detection of GRIN2B/NMDAR2B antibody by Western Blot

Detection of GRIN2B/NMDAR2B by Western Blot

Western blot of 10 μg of rat brain hippocampus lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA Receptor. The labeling by the antibody was blocked by the C-terminal fusion protein of the NR2B subunit of the NMDA Receptor used as immunogen (not shown).

Applications for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2000 dilution
Sample: Frozen sections; unpublished observations

Immunoprecipitation

3 μL/200 μg lysate

Western Blot

1:1000 dilution
Sample: Rat brain (hippocampus) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.

Formulation

10 μg antibody per vial; lyophilized in 5 mM ammonium bicarbonate.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

The lyophilized product is stable at ≤- 20° C for at least 1 year. After reconstitution the antibody should be aliquoted and stored at ≤ -20° C.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GRIN2B/NMDAR2B

Rat NMDA receptor subunit 2B (NR2B) is a 1456 amino acid, 180 kDa, 3-transmembrane member of the glutamate-gated ion channel family. NMDA receptors are heteromultimers of at least two NR1 subunits and two NR2 or two (presumably) NR3 subunits. Upon ligand binding, NMDA receptors open and allow cation transit. The NR1 subunits bind glycine and are essential for the physical formation of the ion channel. NR2 subunits apparently bind glutamate and impart pharmacological properties to the receptor complex. Fully functional receptors require the presence of both glycine and glutamate. The NR1-NR2B complex has the highest affinity for glutamate. Both NR1 and NR2B subunits can be phosphorylated by protein kinase C (PKC). On NR1, PKC phosphorylates on serine at 890; on NR2B, PKC phosphorylates on tyrosine at 1472. Phosphorylation is considered to promote ion transit and impact NR2B interaction with downstream signaling molecules.

References

  1. Grosshans, D.R. and M.D. Browning (2001) J. Neurochem. 76:737.
  2. Cheung, H. et al. (2003) J. Neurochem. 86:1441.
  3. Gibb, A.J. (2004) Trends Neurosci. 27:7.
  4. Liu, Y. and J. Zhang (2000) Chin. Med. J. 113:948.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B

Alternate Names

NMDA R, NR2B Subunit, NMDAR2B, NR2B

Entrez Gene IDs

2904 (Human)

Gene Symbol

GRIN2B

Additional GRIN2B/NMDAR2B Products

Product Documents for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

For research use only

Citations for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

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Protocols

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