Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

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PPS013
Detection of GRIN2B/NMDAR2B by Western Blot
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Product Details
Citations (4)
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Human/Mouse/Rat GRIN2B/NMDAR2B Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Human, mouse, rat ~180 kDa NR2B-subunit of the NMDA Receptor
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Fusion protein from the C-terminus of the NR2B-subunit of rat NMDA Receptor
Formulation
10 μg antibody per vial; lyophilized in 5 mM ammonium bicarbonate.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1:1000 dilution
See below
Immunohistochemistry
1:1000 - 1:2000 dilution
Frozen sections; unpublished observations
Immunoprecipitation
3 μL/200 μg lysate
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of GRIN2B/NMDAR2B antibody by Western Blot View Larger

Detection of GRIN2B/NMDAR2B by Western Blot Western blot of 10 μg of rat brain hippocampus lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDA Receptor. The labeling by the antibody was blocked by the C-terminal fusion protein of the NR2B subunit of the NMDA Receptor used as immunogen (not shown).

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Preparation and Storage

Reconstitution
This antibody should be reconstituted in 50 μL phosphate buffered saline (137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
The lyophilized product is stable at ≤- 20° C for at least 1 year. After reconstitution the antibody should be aliquoted and stored at ≤ -20° C.

Background: GRIN2B/NMDAR2B

Rat NMDA receptor subunit 2B (NR2B) is a 1456 amino acid, 180 kDa, 3-transmembrane member of the glutamate-gated ion channel family. NMDA receptors are heteromultimers of at least two NR1 subunits and two NR2 or two (presumably) NR3 subunits. Upon ligand binding, NMDA receptors open and allow cation transit. The NR1 subunits bind glycine and are essential for the physical formation of the ion channel. NR2 subunits apparently bind glutamate and impart pharmacological properties to the receptor complex. Fully functional receptors require the presence of both glycine and glutamate. The NR1-NR2B complex has the highest affinity for glutamate. Both NR1 and NR2B subunits can be phosphorylated by protein kinase C (PKC). On NR1, PKC phosphorylates on serine at 890; on NR2B, PKC phosphorylates on tyrosine at 1472. Phosphorylation is considered to promote ion transit and impact NR2B interaction with downstream signaling molecules.

References
  1. Grosshans, D.R. and M.D. Browning (2001) J. Neurochem. 76:737.
  2. Cheung, H. et al. (2003) J. Neurochem. 86:1441.
  3. Gibb, A.J. (2004) Trends Neurosci. 27:7.
  4. Liu, Y. and J. Zhang (2000) Chin. Med. J. 113:948.
Long Name
Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B
Entrez Gene IDs
2904 (Human)
Alternate Names
DEE101;GluN1;Glutamate receptor;Glutamate receptor ionotropic, NMDA 1;GRIN1;MRD8;NDHMSD;NDHMSR;NMDA1;NMDAR1;NMD-R1;NR1; GRIN2B; NMDA R, NR2B Subunit; NMDAR2B; NR2B

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Citations for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Altered hippocampal plasticity by prenatal kynurenine administration, kynurenine-3-monoxygenase (KMO) deletion or galantamine
    Authors: C.M. Forrest, K. McNair, M. Pisar, O.S. Khalil, L.G. Darlington, T.W. Stone
    Neuroscience
  2. Changes in synaptic transmission and protein expression in the brains of adult offspring after prenatal inhibition of the kynurenine pathway
    Authors: C.M. Forrest, O.S. Khalil, M. Pisar, K. McNair, E. Kornisiuk, M. Snitcofsky et al.
    Neuroscience
  3. Prenatal activation of Toll-like receptors-3 by administration of the viral mimetic poly(I:C) changes synaptic proteins, N-methyl-D-aspartate receptors and neurogenesis markers in offspring.
    Authors: Forrest, Caroline, Khalil, Omari S, Pisar, Mazura, Smith, Robert A, Darlington, Lynda Ga, Stone, Trevor W
    Mol Brain, 2012-06-09;5(0):22.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Reduced retinal function in amyloid precursor protein-over-expressing transgenic mice via attenuating glutamate-N-methyl-d-aspartate receptor signaling.
    Authors: Shimazawa M, Inokuchi Y, Okuno T, Nakajima Y, Sakaguchi G, Kato A, Oku H, Sugiyama T, Kudo T, Ikeda T, Takeda M, Hara H
    J. Neurochem., 2008-08-07;107(1):279-90.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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