Human/Mouse/Rat HSPH1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human, Mouse, and Rat HSPH1 by Western Blot.
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Human/Mouse/Rat HSPH1 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Detects human, mouse, and rat HSPH1 in Western blots.
Monoclonal Mouse IgG1 Clone # 531104
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human HSPH1
Accession # Q92598
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat HSPH1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat HSPH1 by Western Blot. Western blot shows lysates of HT-29 human colon adeno­carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line untreated (-) or treated (+) with heat shock at 42 °C for 30 minutes and allowed to recover at 37 °C for 3 hours. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSPH1 Monoclonal Antibody (Catalog # MAB4029) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPH1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HSPH1

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Heat shock protein H1 (HSPH1), also known as HSP105 and HSP110, exists as two isoforms, alpha and beta. HSPH1 alpha consists of 858 amino acids whereas HSPH1 beta has 814 amino acids. alpha and beta have calculated molecular weights of 96.4 kDa and 92 kDa, respectively, but an apparent molecular weight of 105‑110 kDa in SDS‑PAGE. HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells. HSPH1 alpha and beta isoforms share 94% identity. Rat HSPH1 has 92% and 97% identity to human and mouse HSPH1, respectively.

Long Name
Heat Shock Protein H1
Entrez Gene IDs
10808 (Human); 288444 (Rat)
Alternate Names
Antigen NY-CO-25; heat shock 105kD beta; heat shock 105kDa protein 1; heat shock 105kDa/110kDa protein 1; Heat shock 110 kDa protein; heat shock protein 105 kDa; HSP105a; HSP105B; HSP105heat shock 105kD alpha; HSP110; HSPH1; KIAA0201DKFZp686M05240; NY-CO-25

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human/Mouse/Rat HSPH1 Antibody
By Anonymous on 03/09/2019
Application: ICC/IF Sample Tested: Embryonic stem cells Species: Mouse

Mouse ESCs were fixed with 4% paraformaldehyde freshly cracked with NaOH, permeabilized with 0.3% Triton, and washed extensively with DPBS. They were blocked with 1% normal goat serum and 5% BSA, then incubated with 1:200 Hsph1 antibody overnight. After washing, Alexa Fluor 488 anti-mouse antibody was added, and nuclei were stained with NucBlue. As expected, Hsph1 localized to the cytoplasm and was more expressed in round ESC colonies than flattened ones.