Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 531104
View all HSPH1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSPH1
Lys388-Glu505
Accession # Q92598

Specificity

Detects human, mouse, and rat HSPH1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat HSPH1 Antibody

Detection of Human, Mouse, and Rat HSPH1 antibody by Western Blot.

Detection of Human, Mouse, and Rat HSPH1 by Western Blot.

Western blot shows lysates of HT-29 human colon adeno­carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Nb2-11 rat lymphoma cell line untreated (-) or treated (+) with heat shock at 42 °C for 30 minutes and allowed to recover at 37 °C for 3 hours. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSPH1 Monoclonal Antibody (Catalog # MAB4029) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for HSPH1 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human/Mouse/Rat HSPH1 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HT‑29 human colon adenocarcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and Nb2‑11 rat lymphoma cell line treated by heat shock

Reviewed Applications

Read 1 review rated 5 using MAB4029 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSPH1

The heat shock proteins (HSPs) are a highly conserved family of stress response proteins. HSPs function primarily as molecular chaperones, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced in response to various types of environmental stresses like heat, cold, and oxygen deprivation. Heat shock protein H1 (HSPH1), also known as HSP105 and HSP110, exists as two isoforms, alpha and beta. HSPH1 alpha consists of 858 amino acids whereas HSPH1 beta has 814 amino acids. alpha and beta have calculated molecular weights of 96.4 kDa and 92 kDa, respectively, but an apparent molecular weight of 105‑110 kDa in SDS‑PAGE. HSPH1 acts as a chaperone to prevent thermal aggregation in mammalian cells. HSPH1 alpha and beta isoforms share 94% identity. Rat HSPH1 has 92% and 97% identity to human and mouse HSPH1, respectively.

Long Name

Heat Shock Protein H1

Alternate Names

HSP105a, HSP110, NY-CO-25

Entrez Gene IDs

10808 (Human); 288444 (Rat)

Gene Symbol

HSPH1

UniProt

Q92598

Additional HSPH1 Products

Product Documents for Human/Mouse/Rat HSPH1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat HSPH1 Antibody

For research use only

Related Research Areas

Heat Shock Proteins (HSPs)

Citations for Human/Mouse/Rat HSPH1 Antibody

Customer Reviews for Human/Mouse/Rat HSPH1 Antibody (1)

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  • Human/Mouse/Rat HSPH1 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Embryonic stem cells
    Species: Mouse
    Verified Customer | Posted 03/09/2019
    Mouse ESCs were fixed with 4% paraformaldehyde freshly cracked with NaOH, permeabilized with 0.3% Triton, and washed extensively with DPBS. They were blocked with 1% normal goat serum and 5% BSA, then incubated with 1:200 Hsph1 antibody overnight. After washing, Alexa Fluor 488 anti-mouse antibody was added, and nuclei were stained with NucBlue. As expected, Hsph1 localized to the cytoplasm and was more expressed in round ESC colonies than flattened ones.
    Human/Mouse/Rat HSPH1 Antibody MAB4029

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