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Human/Mouse/Rat MEKK2 Alexa Fluor® 532-conjugated Antibody

Catalog #: FAB7128X Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB7128X-100UG

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Human/Mouse/Rat MEKK2 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects recombinant human MEKK2 in direct ELISA and human, mouse, rat MEKK2 in Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human MEKK1, 3, or 4 is observed.
Source
Monoclonal Mouse IgG2a Clone # 722728
Purification
Protein A or G purified
Immunogen
E. coli-derived recombinant human MEKK2
Asn42-Tyr264
Accession # Q9Y2U5
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MEKK2

MAPK/ERK kinase kinase 2 (MEKK2) is a widely expressed 70 kDa member of the MEKK/STE11 subfamily of Ser/Thr kinases. It contains a PB1 domain (aa 43-122) which mediates dimerization with MEK5, a region with several phosphorylation sites, and a protein kinase domain (aa 357-617) that includes a site for activating phosphorylation at Thr524. Within aa 42-264, human MEKK2 shares 93% aa sequence identity with mouse and rat MEKK2. Activated MEKK2 translocates from the cytosol to the nucleus, where it forms a complex that binds and activates MKK7 in the c-Jun N-terminal kinase (JNK) pathway, relaying mitogenic and stress signals.

Long Name
Mitogen-activated Protein Kinase Kinase Kinase 2
Entrez Gene IDs
10746 (Human); 26405 (Mouse); 171492 (Rat)
Alternate Names
EC 2.7.11.25; MAP/ERK kinase kinase 2; MAP3K2; MAPK/ERK kinase kinase 2; MAPKKK2; MEK kinase 2; MEKK 2; MEKK2; MEKK2B; mitogen-activated protein kinase kinase kinase 2

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